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A novel antimicrobial packaging material was obtained by incorporating cinnamon essential oil/ β -cyclodextrin inclusion complex (CEO/ β -CD-IC) into polylacticacid (PLA) nanofibers via electrospinning technique. The CEO/ β -CD-IC was prepared by the co-precipitation method and SEM and FT-IR spectroscopy analysis indicated the successful formation of CEO/ β -CD-IC, which improved the thermal stability of CEO. The CEO/ β -CD-IC was then incorporated into PLA nanofibers by electrospinning and the resulting PLA/CEO/ β -CD nanofilm showed better antimicrobial activity compared to PLA/CEO nanofilm. The minimum inhibitory concentration (MIC) of PLA/CEO/ β -CD nanofilm against Escherichia coli and Staphylococcus aureus was approximately 1 mg/ml (corresponding CEO concentration 11.35 μg/ml) and minimum bactericidal concentration (MBC) was approximately 7 mg/ml (corresponding CEO concentration 79.45 μg/ml). Furthermore, compared with the casting method, the mild electrospinning process was more favorable for maintaining greater CEO in the obtained film. The PLA/CEO/ β -CD nanofilm can effectively prolong the shelf life of pork, suggesting it has potential application in active food packaging. [ABSTRACT FROM AUTHOR]