Search

• ESR, DSC, and Ranccimat moments were used for the evolution of the induction times. • Relation between free radicals and oxidation products of deodorized oil was obtained. • The combined effect of deodorized time and temperature promoted free radicals growth. • Priority was first to minimize the deodorization temperature, then down the time. • Identifying and quantifying radicals can provide messages for oil moderate refining. Oxidative stability is a key quality characteristic of edible oils, and the oil's antioxidant capacity decreases during the deodorization stage. This study explores the changes in radical formation, molecular structure, oxidative characteristics, fatty acids, and main bioactive compounds in soybean oil during deodorization. The lag phase decreased, whereas the total amount of spins of free radicals increased as the deodorization time increased from 90 to 150 min. The total amount of spins and percentage of alkyl radicals varied dramatically under different times and temperatures (220 ∼ 260 ℃). Results showed that identifying and quantifying the formed radicals can provide useful information for monitoring and controlling oil oxidation in vegetable oil refining systems. Therefore, to control early oxidation events, maximize refined oil product yield, and reduce energy consumption in the refining plant, the priority should be to minimize temperature during the oil refining process and then shorten the deodorization time. [ABSTRACT FROM AUTHOR]

Tetraselmis chuii is a microalgae marketed as ingredient meeting the acceptance criteria for novel foods established by the European Union and can be an important source of healthy fatty acids (FA). The aim of this research was to characterize the FA profile of T. chuii fractions obtained by supercritical carbon dioxide (SCCO 2) extraction operating with two sequential co-solvents and to evaluate the effect of biomass pretreatment (freeze/thaw cycles followed by ultrasounds). T. chuii biomass was confirmed to be an important source of omega-3 FA, mainly due to the abundance of α-linolenic acid, and pre-treatment significantly improved the lipid yield. Other omega-3 FA, such as 16:3, 16:4, 18:4, 18:5, 20:3 and 20:5, were also detected in different proportions. When SCCO 2 extraction of pretreated and un-pretreated T. chuii was compared with conventional solvent extraction, the nutritional quality indices of the extracts were improved by the use of SCCO 2. • Tetraselmis chuii was confirmed as an important source of omega-3 fatty acids (FA). • Supercritical CO 2 (SCCO 2) extraction improved the FA profile of T. chuii. • Freeze/thaw cycles combined with ultrasounds increased SCCO 2 extraction yield to 5.2%. • SCCO 2 lipid yield was 0.98% and 4.21% with EtAc and EtOH co-solvents, respectively. • SCCO 2 lipid extracts obtained from pretreated T. chuii contained 44% of omega-3 FA. [ABSTRACT FROM AUTHOR]

In the present study, it was investigated if glucose addition (3 or 5%) to pork stimulates glycoxidation (pentosidine, PEN), glycation (Maillard reaction products, MRP), lipid oxidation (4-hydroxy-2-nonenal, 4-HNE; hexanal, HEX; thiobarbituric acid reactive substances, TBARS), and protein oxidation (protein carbonyl compounds, PCC) during various heating conditions and subsequent in vitro gastrointestinal digestion. An increase in protein-bound PEN level was observed during meat digestion, which was significantly stimulated by glucose addition (up to 3.3-fold) and longer oven-heating time (up to 2.5-fold) of the meat. These changes were accompanied by the distinct formation of MRP during heating and digestion of the meats. Remarkably, stimulated glyc(oxid)ation was accompanied by increased protein oxidation, whereas lipid oxidation was decreased, indicating these reactions are interrelated during gastrointestinal digestion of meat. Glucose addition generally didn't affect these oxidative reactions when pork was packed preventing air exposure and oven-heated until a core temperature of 75 °C was reached. [Display omitted] • Pentosidine content increased following gastrointestinal digestion of heated pork. • Glucose and long heating stimulated pentosidine levels in pork (digests). • High pentosidine levels were accompanied by low lipid- but high protein oxidation. • Low pentosidine levels in digests were seen when meat was packed and mildly heated. [ABSTRACT FROM AUTHOR]

In order to prevent the photooxidation of phytosterols, a new type of Pickering emulsion was developed by regulating the oriented distribution of antioxidants in colloidal lipid particles (CLPs) at the oil-water interface. High-melting-point and low-melting-point lipids were tested to modulate their protective effect against phytosterols photooxidation. Results showed that CLPs could stabilize Pickering emulsion and encapsulate antioxidants, providing a dual functional delivery system for phytosterols protection. The Pickering emulsion formed had a particle size of around 350–820 nm, and the crystallization and melting temperatures of tripalmitin particles were approximately 32 °C and 63.8 °C, respectively. The addition of tributyrin or tricaprylin reduced the crystallization and melting temperatures of Pal CLPs and improved the photooxidation emulsion stability. The prepared Pickering emulsion remained stable for a maximum of 12 days under accelerated light-induced oxidation. Among all formulations, the emulsion primarily composed of tripalmitin CLPs, with added tributyrin and resveratrol, exhibited the highest photooxidation stability. [Display omitted] • Emulsions stabilized with colloidal lipid particles had anti-photooxidation effect. • Low-melting-point lipid could modulate the ability of colloidal lipid particles. • Resveratrol had the highest anti-photooxidation ability in Pickering emulsions. • The colloidal lipid particles were distributed at the oil-water interface. [ABSTRACT FROM AUTHOR]

Edible insects with high fat and phosphorus content are a potential novel source of lecithin, however, studies on their minor lipids are limited. In this study, lecithin was extracted from black soldier fly larvae and yellow mealworm. Herein, the effects of lecithin extraction method, matrix and ultrasound pretreatment were explored based on the fatty acid composition and phospholipid profile with soy lecithin as a reference. The use of a wet matrix and ultrasound pretreatment increased the extraction efficiency of total PLs from both insects. Insect lecithin contained a considerable amount of sphingomyelin compared to soy lecithin. In insect lecithin, a total of 47 glycerophospholipid and sphingomyelin molecular species, as well as four molecular species of fatty acyl esters of hydroxy fatty acid, were detected. This study is the first comprehensive investigation of insects as a new source of lecithin with applications in food, cosmetics and in the pharmaceutical industry. • Lecithin was extracted from a novel source: edible insects (YMW and BSFL). • Phospholipid profile of insect lecithin varied among extraction procedures. • Insect lecithin contained a considerable amount of sphingomyelin. • Unlike soy lecithin, insect lecithin contained more PE than PC. • FAHFA and ceramide showed higher abundance in insect lecithin than soy lecithin. [ABSTRACT FROM AUTHOR]

• Extended food use of oats calls for knowledge on compositions and storage behavior. • Lipids, volatiles and tocopherols varied between 20 pure oat cultivar batches. • Nine-month storage of flour revealed differences in oxidative stability. • Minor correlations between lipids, tocols and volatile oxidation compounds detected. Recent data showing the compositional variation and storage behavior among different oat batches for the purpose of food remains limited. Lipids of twenty oat flour samples of pure cultivars grown in Finland during 2019 were extracted and fractionated into neutral and polar-rich lipids. Flour was stored for nine months, and profiles of volatiles and tocols were analyzed to reveal oxidative stability. The lipid content was 5.9–8.9 g per 100 g of flour [DW] and consisted of 78.7 ± 2.5 % neutral and 21.3 ± 2.5 % polar lipids. Palmitic (16 %), oleic (36 %), and linoleic (39 %) acids were the most abundant fatty acids. Neutral lipids had more oleic and less linoleic and palmitic acids than polar lipids. The fresh samples correlated with tocols, pentanal, 2-pentylfuran, 2-heptanone, nonanal, 2-butanone, and heptanal, while stored samples were associated with 3-octen-2-one, 2-octenal, hexanal, and octanal. Lipid composition and oxidative stability are essential factors for selecting oat batches for food applications. [ABSTRACT FROM AUTHOR]

Models predicting lipid oxidation in oil-in-water (O/W) emulsions are a requirement for developing effective antioxidant solutions. Existing models do, however, not include explicit equations that account for composition and structural features of O/W emulsions. To bridge this gap, a mechanistic kinetic model for lipid oxidation in emulsions is presented, describing the emulsion as a one-dimensional three phase (headspace, water, and oil) system. Variation in oil droplet sizes, overall surface area of oil/water interface, oxidation of emulsifiers, and the presence of catalytic transition metals were accounted for. For adequate predictions, the overall surface area of oil/water interface needs to be determined from the droplet size distribution obtained by dynamic and static light scattering (DLS, SLS). The kinetic model predicted well the formation of oxidation products in both mono- and polydisperse emulsions, with and without presence of catalytic transition metals. • Mechanistic kinetic model describes lipid oxidation in oil-in-water (O/W) emulsions. • Compositional and structural features of O/W emulsions are included in the model. • Droplets with sizes <300 nm need to be accounted for in the model. • Kinetic rates of lipid oxidation vary with the droplet size. • Model provides good predictions for mono-and polydisperse emulsions. [ABSTRACT FROM AUTHOR]

Postharvest harmful pathogenic infestation leads to rapid decay in longan fruit. Compared with P. longanae -infected longans, AEOW alleviated fruit disease severity and diminished the O 2 -. production rate and MDA content. It also increased APX, CAT, and SOD activities, delayed the decrease in the levels of GSH and AsA, as well as the reducing power and DPPH radical scavenging ability, which resulted in a decline in membrane lipid peroxidation in P. longanae -infected longans. Additionally, AEOW reduced LOX, lipase, PI-PLC, PC-PLC, and PLD activities, maintained higher levels of PC, PI, IUFA, USFAs, and U/S, while reducing levels of PA, DAG, SFAs, and CMP. These effects alleviated membrane lipid degradation and peroxidation in P. longanae -infected longans. Consequently, AEOW effectively maintained membrane integrity via improving antioxidant capacity and suppressing membrane lipid peroxidation. This comprehensive coordination of ROS and membrane lipid metabolisms improved fruit resistance and delayed disease development in longans. • AEOW reduced O 2 -. burst, MDA production, and CMP rise in P. longanae -infected longan. • AEOW treatment enhanced the antioxidation capacity of P. longanae -infected longan. • AEOW treatment lowered the membrane lipid peroxidation of P. longanae -infected longan. • AEOW reduced P. longanae -induced fruit disease through enhancing antioxidant system. • AEOW reduced P. longanae -induced fruit disease by maintaining cell membrane integrity. [ABSTRACT FROM AUTHOR]

• Hemoglobin and myoglobin had a significant effect on the lipid profiles. • Glycerophospholipids could serve as potential precursors for the production of aroma compounds. • Nitrosomyoglobin may protect ether phospholipids from oxidation. • Hemoglobin, myoglobin, and hemin significantly enhance the content of key aroma compounds. Heme proteins and their derivatives play important roles in inducing lipid oxidation to produce volatile compounds during bacon drying. This study investigated the effects of heme proteins and their derivatives (hemoglobin, myoglobin, nitrosylmyoglobin, hemin, Fe2+, and Fe3+) on lipid and volatiles profiles in the washed pig muscle (WPM) model. The results of the study indicated that the inducers primarily caused the oxidation of glycerophospholipids. Furthermore, hemoglobin and myoglobin had the most significant impact, and their potential substrates may include PE (O-18:2/20:4), PE (O-18:1/20:4), PC (16:0/18:1), and PE (O-18:2/18:2). Nitrosomyoglobin has limited ability to promote lipid oxidation and may protect ether phospholipids from oxidation. The analysis of the volatiles in the model revealed that heme proteins and their derivatives have the ability to induce the production of key aroma compounds. The descending order of effectiveness in inducing the production of aroma compounds is as follows: hemoglobin, myoglobin, hemin, and nitrosylmyoglobin. The effectiveness of Fe2+ and Fe3+ is similar to that of nitrosylmyoglobin. [ABSTRACT FROM AUTHOR]

Abalone, a highly sought-after aquatic product, possesses significant nutritional value. In this study, the relationship between aroma characteristics and lipid profile of abalone (Haliotis discus hannai) during seasonal fluctuation and thermal processing were profiled via volatolomics and lipidomics. 46 aroma compounds and 371 lipids were identified by HS-SPME-GC–MS and UPLC-Q-Extractive Orbitrap-MS, respectively. Multivariate statistical analysis indicated that carbonyls (aldehydes and ketones) and alcohols were the characteristic aroma compounds of abalone. The fluctuations in the aroma compound and lipid composition of abalone were consistent with the seasonal variation, especially seawater temperature. In addition, based on the correlation analysis, it was found that carbonyls (aldehydes and ketones) and alcohols had a positive correlation with phospholipids (lysophosphatidylethanolamines and lysophosphatidylcholines), while a negative correlation was observed with fatty acyls. These findings suggested that the effect of seasonal variations on the aroma changes of abalone might achieved by modulating the lipids composition of abalone. • Carbonyls and alcohols were the characteristic aroma components of abalone. • The variation of abalone lipid accumulation was consistent with seasonal shifts. • Carbonyls and alcohols were positively correlated with PLs and negatively with FAs. • Seasonal variations in abalone lipid accumulation determined its aroma composition. [ABSTRACT FROM AUTHOR]

Rice bran, recognized for its rich lipids and health-beneficial bioactive compounds, holds considerable promise in applications such as rice bran oil production. However, its susceptibility to lipid hydrolysis and oxidation during storage presents a significant challenge. In response, we conducted an in-depth metabolic profiling of rice bran over a storage period of 14 days. We focused on the identification of bioactive compounds and functional lipid species (25 acylglycerols and 53 phospholipids), closely tracking their dynamic changes over time. Our findings revealed significant reductions in these lipid molecular species, highlighting the impact of rancidity processes. Furthermore, we identified 19 characteristic lipid markers and elucidated that phospholipid and glycerolipid metabolism were key metabolic pathways involved. By shedding light on the mechanisms driving lipid degradation in stored rice bran, our study significantly advanced the understanding of lipid stability. These information provided valuable insights for countering rancidity and optimizing rice bran preservation strategies. [Display omitted] • Conducted a comprehensive analysis on rice bran over 14-days of storage period. • Tracked the dynamic changes of functional lipid molecular species in rice bran. • Identified 19 significantly different lipid species over storage time. • Revealed the central lipid metabolic pathways of rice bran. [ABSTRACT FROM AUTHOR]

Herbal teas and beverages have gained global attention because they are rich in natural bioactive compounds, which are known to have diverse biological effects, including antioxidant and anticarcinogenic properties. However, the lipidomic profiles of herbal teas remain unclear. In this study, we applied an untargeted lipidomics approach using high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry to comprehensively profile, compare, and identify unknown lipids in four herbal teas: dokudami, kumazasa, sugina, and yomogi. A total of 341 molecular species from five major classes of lipids were identified. Multivariate principal component analysis revealed distinct lipid compositions for each of the herbs. The fatty acid α-linolenic acid (FA 18:3) was found to be abundant in kumazasa, whereas arachidonic acid (FA 20:4) was the most abundant in sugina. Interestingly, novel lipids were discovered for the first time in plants; specifically, short-chain fatty acid esters of hydroxy fatty acids (SFAHFAs) with 4-hydroxy phenyl nonanoic acid as the structural core. This study provides insight into the lipidomic diversity and potential bioactive lipid components of herbal teas, offering a foundation for further research into their health-promoting properties and biological significance. [Display omitted] • Lipid fingerprinting of four types of herbs led to the detection of 341 lipid species. • Multivariate analysis revealed distinct lipid compositions for each herbal tea. • Novel short-chain fatty acid esters of hydroxy fatty acids are uncovered in herbs. • The abundance of PUFAs in herbal tea made it an excellent choice as a health promotional drink. [ABSTRACT FROM AUTHOR]

[Display omitted] • PA(37:4), TG(4:0_18:0_18:2), and TG(16:0e_18:2_18:3) might be biomarkers. • PC and PE might play an important role on key odorants. • TG(16:1_18:1_18:2) might be a key substrate for binding key aroma compounds. • Lower water activity contributed to the presence of key aroma compounds. The UHPLCHRMS and Gas Chromatography-Olfactometry-Mass Spectrometry (GC-O-MS) techniques were applied to investigate effects of lipid molecules and heat transfer on the generation of aroma compounds in roasted chicken skin. Nineteen odorants were identified as most important aroma contributors based on odor activity values (OAVs) exceeding 1. Lipidomic analysis identified 3926 lipids in the samples, in which triglycerides (TG), phosphatidylcholine (PC), phosphatidylethanolamine (PE), and ceramide (Cer) had a contribution of 20.63%, 12.46%, 11.95%, and 11.39%, respectively. Furthermore, it was observed that PS(18:3e_22:5) and TG(18:0_18:1_18:1) serve as significant chemical markers for distinguishing chicken skin during the roasting (p < 0.05). TGs, notably TG(16:1_18:1_18:2) and TG(18:1_18:2_18:2), were postulated as key retainers for binding crucial aroma compounds. Meanwhile, PC, PE, and Cer played pivotal roles in aroma compound formation. Additionally, higher thermal conductivity and reduced thermal diffusivity significantly contributed to the formation of key odorants. [ABSTRACT FROM AUTHOR]

• Droplet size and stability of coconut milk depended heavily on the fat content. • The stabilization of coconut milk mainly driven by hydrophobic interaction. • Coconut milk with less than 10% fat content had excellent stability. • Coconut milk with 10% fat content had good digestive properties. The fat in coconut milk contributes to unique flavour, while increasing fat content affects stability of the coconut milk. In this study, coconut water and fat were separated, recombined, and homogenized to obtain coconut milk with different fat contents (0–20 %). Emulsifying properties, stability, and digestibility of coconut milk with different fat contents were comprehensively evaluated. The results showed that as the fat content increased from 0 to 20 %, the droplet size increased from 2.18 to 4.70 μm and the viscosity showed an increasing trend. During storage and freeze–thaw, coconut milk with 5 % and 10 % fat content showed excellent stability. In addition, coconut milk with 10 % fat content had superior fat digestibility, which was related to high affinity of pancrelipase. In short, this study revealed that fat content below 10 % can withstand environmental factors such as storage, lipid oxidation, and freeze–thaw, which can be accurately developed as coconut milk products. [ABSTRACT FROM AUTHOR]

[Display omitted] • The main FX-dependent lipid transport proteins are SR-B1, CD36 and ABCA1. • MA, PA and SA improved the expression of FX-dependent lipid transport proteins. • MZ-FAs hydrophilic colloids exhibit good loading properties for FX. • MZ-FAs hydrophilic colloids remarkably promoted the bioaccessibility of FX. • MZ-FAs carries displayed a high-efficiency in promoting the oral absorption of FX. Growing research confirms that lipid transport proteins play a key role in the trans-intestinal epithelial transport of carotenoids. In this study, to simultaneously improve the digestive stability and intestinal absorption of fucoxanthin (FX), functionalized vectors with a capability of up-regulating the expression of FX-specific lipid transporter proteins was fabricated. The results showed that myristic acid, palmitic acid, and stearic acid effectively promoted FX-specific lipid transporter protein expression and formed stable self-assembly complexes with Millard-modified zein (MZ). The FX was sufficiently encapsulated in the MZ-fatty acid (FA) particles, forming spherical nanoparticles with a "core-shell" structure. Simulated gastrointestinal digestion showed that FA introduction significantly increased the FX bioaccessibility. In vivo results further verified that adding FAs dramatically increased the FX serum response concentration. These findings suggest that incorporating nutrients that can promote lipid transporter protein expression into delivery vehicles should be an effective strategy for improving oral carotenoid absorption. [ABSTRACT FROM AUTHOR]

• High osmotic pressure system facilitated the preparation of reduced-salt salted egg yolk. • The mixed sucrose-phosphate-salt pickling system accelerated salt penetration. • Phosphate promoted the formation of dense and stable network structures in protein. • Small molecular weight protein bound with each other to form large aggregates. • The salted egg yolk of 28 d had loose sandy texture and lower salt content (1.07%). This paper was dedicated to the study of the effect of sucrose-phosphate on aspects of physicochemical properties, lipid distribution and protein structure during the picklig of reduced‐salt salted egg yolk (SEY). This work constructed a reduced‐salt pickling system from a new perspective (promoting osmosis) by using a sucrose-phosphate-salt. Results showed that SEY-28d achieved a desirable salt content (1.07 %), hardness (573.46 g) and springiness (0.65 g). The matured SEY was in excellent quality with orange-red color and loose sandy texture. This was because the lipoprotein aggregated with each other through hydrophobic interaction to form a stable network structure. In addition, the hypertonic environment accelerated salt penetration. These also created good condition for lipid spillage. The results of confocal laser scanning microscope also verified this phenomenon. This work provides important guidance for new reduced‐salt curing of traditional pickled foods, deep processing of SEY, and industry development in the field of poultry egg. [ABSTRACT FROM AUTHOR]

In this study, the oxidation of egg yolk lipids (EYL) by salt-induced heat and non-heat treatments was investigated for quality and flavor. The correlation between physicochemical properties, lipid oxidation and antioxidant activity was modeled using partial least squares discriminant analysis (PLS-DA). The results indicated that the prolonged salt-induced synergistic heat treatment produced the highest level of lipid oxidation, antioxidant activity and oil exudation, along with the lowest level of polyunsaturated fatty acid content. In addition, higher contents of pyrazines and fewer acid species were detected, which was not the case with the salt-free heat treatment. In total, 14 identical volatile organic compounds (VOCs) were produced, yet their overall flavor profiles determined by the electronic nose would remain dramatically distinguished. Therefore, heat treatment was particularly critical for lipid oxidation and the generation of aromatic compounds, implying that heat-treated EYL induced by salt is a flavor component with good antioxidant potential. [Display omitted] • Egg yolk lipid (EYL) has great application potential in flavor processing. • The antioxidant properties, structure and flavor release of EYL were analyzed. • Lipid oxidation significantly affected fatty acid composition and flavor profile. • EYL with salt-induced synergistic heat treatment had the best antioxidant capacity. [ABSTRACT FROM AUTHOR]

Lipids are vital precursors to beef aroma compounds, but the exact lipid molecules influencing aroma generation remain unconfirmed. This study employs gas chromatography–olfactometry–mass spectrometry and absolute quantitative lipidomics to identify beef's aroma and lipid profiles and to examine lipid alterations post-thermal processing. The aim is to understand the role of lipids in aroma generation during beef's raw-to-cooked transition. Eighteen key aroma compounds were identified as significant contributors to the aroma of beef. 265 lipid molecules were quantified accurately, and we found that triglycerides containing C18:1 or C18:2 chains, such as TG(16:0_18:1_18:1), TG(16:0_18:1_18:2), TG(16:0_16:1_18:1), as well as phosphatidylcholine and phosphatidylethanolamine containing PC(16:1e_20:4), PC(16:0e_20:4), PC(18:2e_18:2), and PE(16:1e_20:4), played important roles in the generation of key aroma compounds in beef. C18:1, C18:2, C18:3, and C20:4 were key substrates for the formation of aroma compounds. In addition, lysophosphatidylcholine and lysophosphatidylethanolamine containing unsaturated fatty acid chains may serve as important aroma retainers. • Dissecting lipids' role in aroma generation during beef's raw-to-cooked transition. • 18 key aroma compounds in beef were identified using GC–O–MS and OAV > 1. • TGs with C18:1 or C18:2 chains played important roles in aroma compound generation. • LPC and LPE with UFA chains were suggested as important aroma retention agents. • C18:1, C18:2, C18:3, and C20:4 were key substrates in aroma compound generation. [ABSTRACT FROM AUTHOR]

Three-dimensional (3D) printing, as an emerging digital production technology, has recently been receiving increasing attention in food processing. It is important to understand the effect of key ingredients of food materials on the printing, which makes it possible to achieve a wider range of structures using few nozzles and to provide tailored nutrition and personalization. This comprehensive review delves into the latest research on 3D-printed lipid-based foods, encompassing a variety of products such as chocolate, processed cheese, as well as meat. It also explores the development and application of food bioinks that incorporate lipids as a pivotal component, including those based on starch, protein, oleogels, bigels, and emulsions, as well as emulsion gels. Moreover, this review identifies the current challenges and presents an outlook on future research directions in the field of 3D food printing, especially the research and application of lipids in food 3D printing. • 3D food printing technology and materials were described. • The research and application of lipid-based materials were reviewed. • Lipid-incorporated materials as 3D printing inks were reviewed. • The trends of Lipids in 3D food printing were envisioned. [ABSTRACT FROM AUTHOR]

This study was conducted to comprehensively characterize, metabolites, lipids, and volatile flavor compounds of NingXiang (NX) pigs, Berkshire (BKS) pigs, and their crossbred (Berkshire × Ningxiang, BN) pigs using multi-omics technique. The results showed that NX had high intramuscular fat (IMF) content and meat redness. The metabolite and lipid compositions were varied greatly among three pig breeds. The NX pigs exhibited distinctive sweet, fruity, and floral aroma while BN pigs have inherited this flavor profile. 2-pentylfuran, pentanal, 2-(E)-octenal, and acetic acid were the key volatile flavor compounds (VOC) of NX and BKS pork. The VOCs were influenced by the composition and content of metabolites and lipids. The NX pigs have excellent meat quality traits, unique flavor profiles, and high degree of genetic stability regarding flavor. The study deepens our understanding of the flavor of Chinese indigenous pigs, providing theoretical basis to understand the meat flavor regulation under different feeding conditions. • Differential analysis of flavor precursors and volatile flavor compounds in Ningxiang, Barkshire and crossbred pork using multi-omics techniques. • Ningxiang and Barkshire pork differed considerably in sweet, fruity, and floral flavor profiles. • Ningxiang pigs have high genetic stability in flavor. • 2-Pentylfuran, glutaraldehyde, (E)-2-octenal and acetic acid are the key volatile flavor compounds distinguishing the pork types. • Volatile flavor compounds were influenced by the composition and content of metabolites and lipids. [ABSTRACT FROM AUTHOR]

The major lipids and antioxidant activities of Asterias rolleston gonad lipids were evaluated systematically. Major lipids of A. Rolleston gonad lipids were triacylglycerols (TAGs) and phospholipids (PLs). Total lipids were composed of 15.62% of polyunsaturated fatty acids (PUFAs), and 40.81% of monounsaturated fatty acids (MUFAs). The most abundant PUFA were C20:5n-3 (EPA) (6.28%) and C22:6n-3 (DHA) (5.80%). Predominantly composed of phosphatidylcholine (PC) and phosphatidylethanolamine (PE), polar lipids were rich in PUFAs and could contain up to 34.59% EPA and DHA, and PE and PI (phosphatidylinositol) were also found to be the main carriers of EPA and ARA (arachidonic acid) in polar lipids. The MUFA and PUFA of Sn -2 in TAG are 39.72% and 30.37%, respectively. A total of 64 TAG species were identified, with Eo-P-M, Eo-Eo-M, and M-M-Eo being the main TAGs components. Moreover, A. rollestoni gonad lipids exhibited potent radical scavenging activities and reducing power in a dose-dependent manner. • A. rollestoni Gonad Lipids: In-depth characterization unveils structural insights. • n-3 Rich Lipids: Abundance of EPA and DHA for superior health benefits. • Phospholipid Wealth: PE and PI are the main carriers of n-3 PUFAs. • Powerful Antioxidants: Robust scavenging activities for lipid applications. [ABSTRACT FROM AUTHOR]

This study explored how co-oleogelator type, concentration, and water addition affect lipid digestion and β-carotene (βC) bioaccessibility in corn oil oleogels. Oleogels containing 0.1% βC, 20% glyceryl stearate (GS), with lecithin (L) or hydrogenated lecithin (HL) (at 0, 0.5, or 2.5%) and their water-filled counterparts (1% water) were examined. In vitro intestinal digestion revealed HL-oleogels experienced higher lipolysis due to their smaller crystal size enhancing surface area for lipase action, whereas L-oleogels presented lower digestibility, attributed to larger oil droplets and a minimized surface area. Water addition didn't significantly change lipid digestibility. βC bioaccessibility was inversely related to co-oleogelator concentration, with L-oleogels demonstrating the largest decrease, likely due to less free fatty acids released for micelle formation. However, water-filled oleogels enhanced βC bioaccessibility. These findings highlight that tailored microstructure in oleogels can control lipid digestion and βC bioaccessibility, paving the way for designing efficient delivery systems for targeted nutrient delivery. • Co-oleogelator Types Alter Oleogel Structure & Nutrient Access. • Lecithin (L) Reduces Oleogel In Vitro Lipid Digestibility. • Hydrogenated Lecithin (HL) Boosts Lipid Digestibility. • L and HL Decreases β-Carotene Bioaccessibility in Oleogels But Water Increases It. [ABSTRACT FROM AUTHOR]

This study aimed to conduct a comparative analysis of germinated seeds and microgreens derived from Perilla frutescens var. crispa f. viridis, hypothesizing that microgreens would exhibit higher concentrations of nutrients and bioactive compounds compared to their precursors. Perilla frutescens was chosen for its popularity and wide use in Asian cuisine. A series of analytical methods was employed to quantify and qualify various components. The findings indicate that germinated seeds exhibit significantly higher quantities of lipids, proteins, sugars, free amino acids, and minerals, whereas microgreens possess significantly high concentration of vitamins and polyphenols. These results provide valuable insights into the nutritional differences between germinated seeds and microgreens, highlighting their distinct contributions to diet. Specifically, incorporating germinated seeds can enhance macronutrient intake, while microgreens can boost antioxidant intake. These findings can inform the development of targeted dietary recommendations, promoting the inclusion of both germinated seeds and microgreens to meet specific nutritional needs and improve health outcomes. • Microgreens and germinated seeds exhibit distinct macro- and micro-nutrient compositions. • Microgreens produced without a growth medium have lower macronutrient content. • Germinated seeds contain only vitamin E, microgreens are rich in polyphenols and other vitamins. • Growth medium used for microgreens significantly influences their nutrient composition. • Carotenoids and polyphenols concentration raises from the gemination to adult plant stage. [ABSTRACT FROM AUTHOR]

The study investigated the lipid oxidation of pumpkin seed kernels (PSK) under different storage conditions (room temperature, vacuum-room temperature, refrigeration, and vacuum-refrigeration) using HPLC-MS and GC–MS. Experimental results found the vacuum-refrigeration group showed the lowest PV (0.24 g/100 g), diene (8.68), hexanal (356.64 ± 16.06 ng/g), and nonanal (132.05 ± 8.38 ng/g) after a 9-month storage. A total of 586 lipids, including 6 classes and 27 subclasses, were detected, 46 of which showed significant differences. Refrigeration samples had the highest diacylglycerol content, while room temperature samples demonstrated the highest triacylglycerol and phosphatidylcholine content. Differential lipid metabolite analyses indicated that storage conditions mainly affected glycerolipid metabolism, glycerophospholipid metabolism, and sphingolipid metabolism pathways in PSK, while glycerolipid and glycerophospholipid metabolism were still dominant. It revealed that refrigeration was more effective than vacuum in inhibiting the oxidation of PSK. These findings could offer valuable references for the storage, transportation, preservation, and the development and utilization of PSK. [Display omitted] • Aldehydes and alcohols were main differential flavor compounds in PSK. • Storage conditions significantly impacted the lipid oxidation degree of PSK. • 589 lipid metabolites, including 6 classes and 27 subclasses, were identified. • Lipid metabolism pathways in PSK under different storage conditions were revealed. [ABSTRACT FROM AUTHOR]

[Display omitted] • NBT significantly decrease physical stability of BS-stabilized lipid droplets. • NBT increased the size of lipid droplets and reduced the contact area between lipase and lipid. • The interaction between NBT and BS at the oil–water interface was measured by ITC and MD. Previous lipase inhibitors studies mainly focus on the binding between inhibitors and lipase, ignoring the impact of inhibitors on the oil–water interface of lipid droplets. This study aimed to investigate the effect of nobiletin (NBT) from Citri Reticulatae Pericarpium on the oil–water interface properties and lipid digestion. Here, we found that NBT could destroy bile salt (BS)-stabilized lipid droplets and thus inhibited free fatty acid release, owing to the interaction between NBT and BS at the oil–water interface, and reducing the stability of the oil–water interface (the stability index decreased from 91.15 ± 2.6 % to 66.5 ± 3.6 %). Further, the molecular dynamics simulation and isothermal titration calorimetry revealed that NBT could combine with BS at oil–water interface through intermolecular interactions, including hydrogen bonds, Van der Waals force, and steric hindrance. These results suggest that the interfacial instability of NBT mediated BS emulsified oil droplets may be another pathway to inhibit lipid digestion. [ABSTRACT FROM AUTHOR]

• Application of structured illumination microscopy to examine milk ultrastructure. • Pasteurization significantly impacts PC and SM in the outer membrane. • Heat treatment reduces glycerolipids with AA/DHA and glycerophospholipids. • Addition of phospholipids and freeze-drying enhance polar lipid content. • SIM effectively displays nanoscale changes in fat globules during processing. The introduction of exogenous lipids in the production of infant formula induces significant alterations in milk lipid composition, content, and membrane structure, thus affecting the lipid digestion, absorption, and utilization. This study meticulously tracks these changes throughout the manufacturing process. Pasteurization has a significant effect on phosphatidylcholine and sphingomyelin in the outer membrane, decreasing their relative contents to total polar lipids from 12.52% and 17.34% to 7.72% and 12.59%, respectively. Subsequent processes, including bactericidal-concentration and spray-drying, demonstrate the thermal stability of sphingomyelin and ceramides, while glycerolipids with arachidonic acid/docosahexaenoic acid and glycerophospholipids, particularly phosphatidylethanolamine, diminish significantly. Polar lipids addition and freeze-drying technology significantly enhance the polar lipid content and improve microscopic morphology of infant formula. These findings reveal the diverse effects of technological processes on glycerolipid and polar lipid compositions, concentration, and ultrastructure in infant formulas, thus offering crucial insights for optimizing lipid content and structure within infant formula. [ABSTRACT FROM AUTHOR]

[Display omitted] • Potential relationship of polar lipids and oil accumulation was explored in oat and wheat. • Polar lipids were analyzed in developing endosperms of oat and wheat. • Dynamics of 78 molecular species of 11 polar lipid classes have been characterized. • Major differences of lyso phospholipid and glycolipid contents have been revealed. Polar lipids have biosynthetic pathways which intersect and overlap with triacylglycerol biosynthesis; however, polar lipids have not been well characterized in the developing endosperms of oat with high oil accumulation. The polar lipids in endosperms of oat and wheat varieties having different oil contents were analyzed and compared at different developmental stages. Our study shows that the relative contents of polar lipid by mass were decreased more slowly in wheat than in oat. Phosphatidylcholine and phosphatidylethanolamine were the major phospholipids, which showed similar abundance and gradual decreases during endosperm development in oat and wheat, while lyso phospholipids were noticeably higher in oat. Monogalactosyldiacylglycerol showed a gradual increase in wheat and a decrease in oat during endosperm development. The relative contents of some polar lipid species and their unsaturation index were significantly different in their endosperms. These characteristics of polar lipids might indicate an adaption of oat to accommodate oil accumulation. [ABSTRACT FROM AUTHOR]

• Higher contents of aldehydes, ketones, and alcohols were found in the PAW-DBD group. • Alterations in lipid profiles were studied by LC-MS-based lipidomics analyses. • 130 lipid species were identified as DALs. • TG was the major lipid of 130 DALs. • Glycerophospholipid metabolism was the predominant metabolic pathway. To explore the combination effects of plasma-activated water and dielectric barrier discharge (PAW-DBD) cold plasma treatment on the formation of volatile flavor and lipid oxidation in Asian sea bass (ASB), the volatile flavor compounds and lipid profiles were characterized by gas chromatography-ion mobility spectrometry and LC-MS-based lipidomics analyses. In total, 38 volatile flavor compound types were identified, and the PAW-DBD group showed the most kinds of volatile components with a significant (p < 0.05) higher content in aldehydes, ketones, and alcohols. A total of 1500 lipids was detected in lipidomics analysis, phosphatidylcholine was the most followed by triglyceride. The total saturated fatty acids content in PAW-DBD group increased by 105.02 μg/g, while the total content of unsaturated fatty acids decreased by 275.36 μg/g. It can be concluded that the PAW-DBD processing increased both the types and amounts of the volatile flavor in ASB and promoted lipid oxidation by altering lipid profiles. [ABSTRACT FROM AUTHOR]

• Synthesis and nutritional outcomes of OPL are reviewed. • O-L-L, O-M-L, O-P-L are differential TAGs between China and other countries. • Only acidolysis has been used for the synthesis of OPL. • Secondary-feeding acidolysis has great application prospects for OPL synthesis. • Compared to OPO, dietary OPL is associated with increased adipogenesis. Based on multivariate statistics, this review compared major triacylglycerols (TAGs) in animal milk and human milk fat from China and other countries. Human milk fat differs from animal milk fat in that it has longer acyl chains and higher concentrations of 1,3-dioleoyl-2-palmitoyl-glycerol (O-P-O) and 1-oleoyl-2-palmitoyl-3-linoleoylglycerol (O-P-L). O-P-L is a significant and distinct TAG in human milk fat, particularly in China. 1-oleoyl-2-palmitoyl-3-linoleoylglycerol (OPL) is human milk's major triglyceride molecule of O-P-L, accounting for more than 70%. As a result, OPL has piqued the interest of Chinese academics. The synthesis process and nutritional outcomes of OPL have been studied, including changes in gut microbiota, serum lipid composition, improved fatty acid and calcium absorption, and increased total bile acid levels. However, current OPL research is limited. Therefore, this review discussed enzymatic preparation of 1,3-dioleoyl-2-palmitoyl-glycerol (OPO) and OPL and their nutritional and physiological activity to direct future research direction for sn -2 palmitate and OPL. [ABSTRACT FROM AUTHOR]

• The metabolic pathway networks of three energy substances in flaxseed germination were proposed. • Arginine and Proline metabolism were the key pathways in flaxseed during germination. • During flaxseed germination, arginine was the most active metabolite. • Sucrose partakes in galactose metabolism and starch and sucrose metabolism. • The main phospholipid molecules in the flaxseed were PA, PE, LPA, and LPC at 0–7 d. Germinated flaxseed (Linum usitatissimum L.) is an essential potential food ingredient, but the major energy substances (proteins, lipids, and carbohydrates) metabolites and metabolic pathways are unknown. Comprehensive metabolomic analyses were performed using Fourier transform infrared spectroscopy and high-performance liquid chromatography mass spectrometry on flaxseed from 0 to 7 d. Additionally, the critical metabolites pathways networks of three energy substances metabolites during flaxseed germination were exhibited. The results showed that arginine was the most active metabolite during germination, strongly associated with the arginine biosynthesis and arginine and proline metabolism pathways. Carbohydrates predominantly comprised sucrose on 0–3 d, which participated in galactose metabolism and starch and sucrose metabolism. The main flaxseed phospholipid molecules were phosphatidic acid, phosphatidylethanolamine, lysophosphatidic acid, and lysophosphatidylcholine during germination. This study underscores the paramount metabolic pathways in proteins, lipids and carbohydrates were arginine and proline metabolism, linoleic acid metabolism, arachidonic acid metabolism, and ascorbate and aldarate metabolism during germination. [ABSTRACT FROM AUTHOR]

[Display omitted] • The development of peanut oil volatile flavor requires oil to participate in thermal reaction. • Heating peanut oil to degrade the unsaturated fatty acids in the oil creates reducing aldehydes. • Linoleic acid is one of the precursors in the formation of the volatile flavor of peanut oil. • Develop a volatile flavor model capable of distinguishing different reactions. Lipid is an important precursor for volatile flavor formation, but it is not clear how to study the reactions involved in forming key volatile flavor compounds in peanut oil. In this paper, we innovatively established a flavor research model to investigate the contribution of different chemical reactions to the aroma compounds of peanut oil. The results showed that lipid participation in thermal reactions is necessary for forming major aroma compounds in hot-pressed peanut oil. Compared to the Maillard reaction, the lipid oxidation-Maillard reaction produces more compounds with 46 volatile substances identified. During the heating process, six new key substances were formed and the level of unsaturated fatty acids decreased by 7.28%. Among them, linoleic acid may be an important precursor for the formation of aroma components of hot-pressed peanut oil. Our study could provide theoretical guidance for understanding the volatile flavor mechanism of peanut oil and improving volatile flavor. [ABSTRACT FROM AUTHOR]

[Display omitted] • Lipid differences between yak colostrum (YC) and mature milk (YM) were analyzed. • A total of 851 lipid species from 28 lipid subclasses were identified. • Forty-three significantly different lipids (SDLs) between YC and YM were screened. • These SDLs were mainly enriched in the glycerophospholipid metabolic pathway. • Lipid species information for GLs, STs, GPs, SPs, and FAs in YC and YM was added. Yak milk is essential to maintain the normal physiological functions of herders in Tibetan areas of China. However, the lipid components of yak colostrum (YC) and mature milk (YM) have not been systematically studied. We employed a quantitative lipidomics to comprehensively describe the alterations in the milk lipid profile of lactating yaks. Herein, totally 851 lipids from 28 lipid subclasses in YC and YM were identified and screened for 43 significantly different lipids (SDLs; variable importance in projection > 1, fold change < 0.5 or > 2 with P < 0.05), with cholesterol ester (CE, 16:0) and triacylglycerol (TAG, 54:6 (20:5), 50:1 (16:0), 56:6 (20:5)) were the potential lipid biomarkers. Fourteen SDLs were modulated downwards, and 29 SDLs were modulated upwards in YM. Moreover, by analyzing lipid metabolic pathways in these SDLs, glycerophospholipid metabolism was the most critical. Our results furnish integral lipid details for evaluating yak milk's nutritional quality. [ABSTRACT FROM AUTHOR]

• Amylosucrase and pullulanase treatments of amylopectin produced linear α-glucans. • Linear α-glucans were prone to form V-type complexes with unsaturated fatty acid. • Digestibility of the complexes was controlled by their crystalline structure. • The formation of V-type structure improved the stability of unsaturated fatty acid. In this study, waxy corn starch (WCS) was modified by amylosucrase and pullulanase, producing linear starch chains with elongated length that favored the complexation with unsaturated fatty acids (uFAs). Compared to native WCS, the amylosucrase-modified WCS with an average chain length of 47.8 was easier to form V-type complexes with oleic acid, while increasing the degree of unsaturation impeded the formation of V-type complexes. The pullulanase treatment hydrolyzed the branching points of amylosucrase-modified WCS and the linear starch chains could forme V-type complexes with oleic acid, linoleic acid, and linolenic acid, with V-type crystallinity decreasing from 38.2 % to 20.1 %. V-type complexes had a lower thermal stability than the B-type starch crystallites, and their peak melting temperature ranged from 67.2 to 79.0 °C. The content of resistant starch in the complexes was in the range of 21.8 %-40.9 % and the formation of V-type complexes decreased the susceptibility of uFAs to oxygen. [ABSTRACT FROM AUTHOR]

[Display omitted] • Different forms of CBD (isolate or extract) did not affect the characteristics of nanostructures. • Lipid composition of nanostructures did not affect their particle size and crystallization pattern. • All nanostructures were stable during storage with CBD retention rate of 100%. • Soy lecithin and Tween 80 individually were better than their mixture to produce nanostructures. This study evaluated the physicochemical characteristics of nanostructured lipid carriers (NLCs) as a potential vehicle for cannabidiol (CBD), a lipophilic molecule with great potential to promote health benefits. NLCs were produced using hemp seed oil and fully-hydrogenated soybean oil at different proportions. The emulsifiers evaluated were soybean lecithin (SL), Tween 80 (T80) and a mixture of SL:T80 (50:50). CBD was tested in the form of CBD-rich extract or isolate CBD, to verify if it affects the NLCs characteristics. Based on particle size and polydispersity, SL was considered the most suitable emulsifier to produce the NLCs. All lipid proportions evaluated had no remarkable effect on the physicochemical characteristics of NLCs, resulting in CBD–loaded NLCs with particle size below 250 nm, high CBD entrapment efficiency and CBD retention rate of 100% for 30 days, demonstrating that NLCs are a suitable vehicle for both CBD-rich extract or isolate CBD. [ABSTRACT FROM AUTHOR]

• Proteases are mainly involved in the later stages of protein degradation. • γ-glutamyl-peptides, involved in the kokumi taste, increased with ripening. • Ripening enriched the volatilome of Coppa Piacentina in aldehyde and ketone. Coppa Piacentina is an Italian protected designation of origin (PDO) dry-cured product obtained from the muscle of pork neck and ripened for at least six months. Metabolomics- and volatilomics-based strategies, combined with a chemical characterization of free amino acids were applied to identify biomarkers of long-ripened Coppa Piacentina PDO. Long ripening induced a significantly increase of total free amino acids, mainly represented by glutamic acid, involved in the umami taste perception. Untargeted metabolomics, performed using UHPLC-HRMS, allowed to identify 32 putative gamma-glutamyl-peptides, known as main contributors to the kokumi taste. Unsupervised and supervised multivariate statistics observed a clear modification of these peptides over the ripening, with gamma-glutamyl-peptides which significantly increased in long-ripened samples. A volatilomics-based strategy, conducted with GCxGC-MS, was then performed, and 93 different compounds were identified, with aldehyde and ketones deriving from the lipid auto-oxidation which increased according to ripening. [ABSTRACT FROM AUTHOR]

[Display omitted] • New, simple and quick IL-OTTPTME technique for fatty food matrices was developed. • IL-OTTPTME technique can extract analytes while eliminating lipid co-extractives. • Infinite contact and strong interaction between IL and analyte lead to ER of ∼ 100 %. • Formation of three-phase system allows the elimination of lipid co-extractives. • The work offers a new way for accurate analysis of trace analytes in fatty matrices. Extraction of trace contaminants from fatty food matrices is challenging in food analysis. Herein, a new ionic liquid-based one-, two-, three-phase transition microextraction (IL-OTTPTME) was proposed to efficiently extract trace targets while simultaneously eliminating lipid co-extractives. The method performance was illustrated through the determination of chrysoidine in fatty soybean products using high-performance liquid chromatography-ultraviolet/visible detection. The strong interactions and infinite contact between IL and chrysoidine in the one-phase system ensured ultra-high extraction efficiency (∼100 %). Density functional theoretical calculations confirmed the presence of strong hydrogen bonding and π-π interactions. The formation of the three-phase system during extraction could completely eliminate lipid co-extractives. The IL-OTTPTME integrated extraction, enrichment and cleanup steps into one step, making it rapid and extremely easy to operate. The method had a wide linear range of 0.5–5000 μg/kg and low limit of detection (0.15 μg/kg). It also had satisfactory relative recoveries (95.1 %-104.0 %) and low RSDs (≤5.0 %, n = 5). [ABSTRACT FROM AUTHOR]

[Display omitted] • α-Tocopherol loaded solid lipid nanoparticles formulated with hardfats are stable for physical and crystallization characteristics. • α-Tocopherol contributed to particle stability of lipids with similar chain length to their composition. • α-Tocopherol incorporation modify the crystallinity of solid lipid nanoparticles. • Solid lipid nanoparticles proved to be effective for incorporating α-tocopherol. Solid lipid nanoparticles can be compatible with several bioactive compounds and confer a differentiated crystalline structure. This study aimed to produce α-tocopherol loaded solid lipid nanoparticles with fully hydrogenated oils and fats from palm oil, soybean oil, and crambe oil, by high-pressure homogenization, using lecithin as an emulsifier. After recrystallization of solid lipid nanoparticles, dispersions were evaluated until 60 days of storage for particle size, polydispersity index, zeta potential, microstructure, dispersion stability and α-tocopherol quantification. α-tocopherol loaded solid lipid nanoparticles showed particle sizes and zeta potential values considered adequate for this type of particle. Presence of α-tocopherol altered thermal behavior of the particles, leading to increased crystallinity, with no changes in polymorphism, when compared to the unloaded solid lipid nanoparticles. All α-tocopherol loaded solid lipid nanoparticles dispersions showed stability with no losses of α-tocopherol, indicating their potential as a carrier for this compound in fortified foods. [ABSTRACT FROM AUTHOR]

• VOs were divided into herbaceous VOs, gramineous VOs, vine VOs, and woody VOs. • The detection technologies of VOs in recent years were preliminarily integrated. • Nondestructive rapid detection technology is an important analysis method for VOs. • Lipidomics technology has broad application prospects in the field of VOs. Lipids are widespread in nature and play a pivotal role as a source of energy and nutrition for the human body. Vegetable oils (VOs) constitute a significant category in the food industry, containing various lipid components that have garnered attention for being natural, environmentally friendly and health-promoting. The review presented the classification of raw materials (RMs) from oil crops and quality analysis techniques of VOs, with the aim of improving comprehension and facilitating in-depth research of VOs. Brief descriptions were provided for four categories of VOs, and quality analysis techniques for both RMs and VOs were generalized. Furthermore, this study discussed the applications of lipidomics technology in component analysis, processing and utilization, quality determination, as well as nutritional function assessment of VOs. Through reviewing RMs and quality analysis techniques of VOs, this study aims to encourage further refinement and development in the processing and utilization of VOs, offering valuable references for theoretical and applied research in food chemistry and food science. [ABSTRACT FROM AUTHOR]

Okara, the solid byproduct of water-soluble soybean extract production, is a potential source of proteins and isoflavones. This study investigated different experimental configurations for extracting these compounds from wet okara, including lipid removal with ethanol at different stages of the recovery process, sequential crosscurrent extraction, and using a saline MgCl 2 solution as the solvent. Three washes with a 60:40 ethanol:water (w/w) solution after isoelectric precipitation significantly increased protein content by reducing lipid content (60 %). The crosscurrent approach using 0.05 M MgCl 2 yielded okara proteinaceous material with 248 µg/g daidzein and 236 µg/g genistein, along with a 3 % increase in protein content, attributed to enhanced extraction of 7S globulins. These configurations notably affected the functional properties of the protein materials. Overall, this research provides detailed insights into the composition and properties of proteins extracted from wet okara, facilitating their specialized application in food products., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Effects of heat treatment (100 °C) at different moisture content (13-70 %) on the structural, gelation and digestive properties of starch in real mung bean flour (MBF) systems are investigated. The results showed that the structural destruction of the starch, the starch-lipid complexion and starch-protein interaction were promoted with increasing moisture content. The starch-protein interaction was mainly driven by hydrophobic interaction forces, leading the increase of total phase transition enthalpy. Even though starch retained ordered structure after heating at 50 %-70 % moisture, the typical pasting curve almost disappeared. The less leached amylose to construct the continuous phase, and more flexible amylopectin swollen granules dispersed in the matrix may weakened the viscoelasticity of the gels. As a result, two distinct gel textures were presented: soft solids with good water-binding capacity (below 30 %) and pasty fluids (above 40 %). Starch-lipid/protein interactions were demonstrated to retard the digestion rate of starch during MBS gelatinization according to the two-stage first-order kinetic and LOS (logarithm of the slope) models., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Ltd.)

Fucoxanthin is a xanthophyll carotenoid that possesses potent antioxidant, anti-obesity, and anti-tumor properties. However, its limited solubility in water and susceptibility to degradation create challenges for its application. In this study, a microfluidic coaxial electrospinning technique was used to produce core-shell zein-gelatin nanofibers for encapsulating fucoxanthin, enhancing its bioavailability, and improving its stability. In comparison to uniaxially-loaded fucoxanthin nanofibers, the encapsulation efficiency of fucoxanthin reached 98.58 % at a core-shell flow rate ratio of 0.26:1, representing a 14.29 % improvement. The photostability of the nanofibers increased by 74.59 % after three days, UV stability increased by 38.82 % after 2 h, and temperature stability also significantly improved, demonstrating a protective effect under harsh environmental conditions (P < 0.05). Additionally, nanofibers effectively alleviated oleic acid-induced reactive oxygen species production and reduced fluorescence intensity by 54.76 %. MTT experiments indicated great biocompatibility of the nanofibers, effectively mitigating mitochondrial membrane potential polarization and lipid accumulation in HepG2 cells. Overall, the microfluidic coaxial electrospinning technique enables promising applications of fucoxanthin delivery in the food industry., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Eel is a commercially important marine fish, frequently featured as sushi or roasted preparations. This study determined the formation of heterocyclic amines (HAs) and advanced glycation end products (AGEs) in roasted eel and evaluated the inhibitory mechanism of quercetin and l-ascorbic acid on their formation. The results indicate a respective reduction of 75.07% and 84.72% in total HAs, alongside a decline of 23.03% and 39.14% in AGEs. Additionally, fundamental parameters of roasted eel, lipid oxidation indicators and precursors were measured to elucidate the mechanisms and impact of natural antioxidants on HAs and AGEs formation in roasted eel. Furthermore, endeavors were made to probe into the molecular mechanisms governing the influence of key differential lipids on the generation of HAs and AGEs through lipid-mics analysis. This research emphasizes the potential of natural antioxidants in preventing harmful substances formation during eel thermal processing, which is helpful to food manufacturers for healthier food production., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Anthocyanins (ACNs) systems encapsulated in nanomaterials have received widespread attention and rapid development due to its good delivery potential. Here, the favorable benefits of four natural polysaccharide food additives coated ACNs-liposome nanoparticles (ACNs-Lipo NPs) on the stability and possible lipid-lowering effects of ACNs are discussed in this work. The polysaccharides were coupled to the ACNs-Lipo NPs and self-assembled to create ACNs-Lipo@polysaccharide NPs. The impact of various polysaccharides on the physical, chemical, and stability characteristics of NPs was examined. We found that the NPs prepared with gum arabic (GA) had the best stability. FT-IR and XRD analysis revealed electrostatic adsorption and hydrogen binding forces between the components, as well as an amorphous structure. A series of tests in vitro confirmed the excellent stability, bioavailability, antioxidant activity, and biocompatibility of NPs. Finally, cellular antioxidant activity (CAA) and oleic acid (OA)-induced lipid deposition cell models revealed that ACNs-Lipo@GA might be more readily absorbed by cells, resulting in improved antioxidant activity and lipid-lowering impact, with possible targeted delivery qualities and lipid-lowering effect., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Glycans in corn silk could interact with co-existing small molecules during its absorption, digestion, and biological process. In order to understand the exact mechanism of action of zeaxanthin, it is critical to investigate the biomolecular interactions, which were necessary to form a glycan-small molecule complex and yet produce the bioactive effect. So far, the in-depth study of these natural interactions has not been fully elucidated. Here, we probed that the molecular interaction between zeaxanthin (ZEA) and glycans from corn silk (CSGs) was driven by enthalpy. More importantly, it was the first time found that CSGs can bind to lipid-soluble ZEA could be binded with CSGs. It was the first report on the thermostability of insulin structure and natural glycans. This study should facilitate our understanding of the interaction between lipid soluble molecules and glycans, and provide a more comprehensive understanding of the nutrient base in food., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

• Two Ulva species were exposed to four salinity levels. • Lipidome was characterised via GC–MS and HILIC-LC-MS. • Profound differences between the two Ulva species were found. • Fatty acids and polar lipids strongly accumulated at low salinity. • Higher nutritional profile of Ulva australis biomass at low salinity. The sea lettuce Ulva spp is becoming an increasingly important macroalgae for aquaculture. Sea lettuce can be grown on- and off-shore, displays high growth rates, and its biomass possesses attractive nutritional benefits. Among those are their fatty acids (FA) and lipid profiles, rich in omega 3 Polyunsaturated Fatty Acids (PUFAs) as well as bioactive lipids. In order to tailor those properties for food applications, we explored the use of a short-term (seven days) low salinity treatment to modulate the lipid profile of two species of Ulva. We found large quantitative differences between species, and while a low-salinity treatment negatively affected growth, Ulva australis' lipid profile was positively impacted. Total FA particularly ɷ-3 PUFAs, increased three-fold, as well as most polar lipid species including known bioactive compounds. This study highlights profound differences between species and describes a simple method to increase the nutritional properties of Ulva biomass for food applications. [ABSTRACT FROM AUTHOR]

• The effect of gamma irradiation on smoked bacon quality during storage was studied. • The artificial neural network was first used to predict irradiated smoked bacon quality. • Gamma irradiation can slow down the quality and sensory degradation of smoked bacon. • BP-ANN has great potential in predicting the gamma-irradiated bacon quality. This study investigated the effect of gamma irradiation on smoked bacon quality during storage and developed a multi-quality prediction model based on gamma irradiation. Gamma irradiation reduced moisture content and improved the microbial safety of smoked bacon. It also accelerated protein and lipid oxidation and altered free amino acids and fatty acids composition. It was effective in slowing down quality deterioration and sensory quality decline during storage. The backpropagation artificial neural network (BP-ANN) model was constructed by using physical and chemical indicators, irradiation dose, and storage time as input variables, and the total number of colonies and sensory scores as output layers. The transfer functions of the input-hidden layer and hidden-output layer were ReLu and Sigmoid, respectively. There were 13 neurons in the hidden layer. Results showed that BP-ANN based on physical and chemical indicators, irradiation dose, and storage time had great potential in predicting the multiple quality of smoked bacon. [ABSTRACT FROM AUTHOR]

[Display omitted] • Endogenous DHA in milk is more bioaccessible than exogenously added DHA. • Endogenous DHA in milk has higher digestive stability than exogenously added DHA. • The bound form of milk endogenous DHA facilitates its digestion and absorption. • Naturally enriched milk fat hydrolyzes easier than artificially enriched milk fat. This study aimed to compare the bioaccessibility of docosahexaenoic acid (DHA) in naturally and artificially enriched milk and investigate the potential mechanisms involved. The results indicated that the DHA in naturally enriched milk (NEM) had a higher bioaccessibility (76.9 %) and a lower digestive loss rate (18.1 %) compared to artificially enriched milk (ArEM). Moreover, NEM contained a higher proportion of DHA-containing glycerophospholipids and sn -2 DHA, with fewer long-chain fatty acids and more saturated fatty acids adjacent to DHA in the same lipid molecule. During simulated intestinal digestion, NEM had a higher free fatty acid release and lipid digestion rate than ArEM. These findings suggested that the bioaccessibility of endogenous DHA in milk was superior to that of externally added DHA due to its more easily digestible and absorbable chemical binding form and lower digestive loss rate. The easy digestibility of milk lipids in NEM also contributed to its high DHA bioaccessibility. [ABSTRACT FROM AUTHOR]

• Chemical as well as physical modification of filler polysaccharides improve whey films. • Organic and inorganic fillers in combination improve packaging properties. • Essential oils are a promising additive for enhancing quality and shelf life of foods. • Various aspects such as bioavailability and use of green techniques should be explored. Whey protein-based biocomposite films (WBF) are gaining significant importance as edible packaging materials due to their eco-friendly, biodegradable and barrier properties. The review aims to explore the impact of different reinforcing agents on the techno-functional properties of WBF. The incorporation of reinforcing agents, such as polysaccharides, lipids, starch, chitosan, cellulose, essential oils, and hydrocolloid gums, plays a crucial role in shaping the techno-functional properties of WBF. The review article suggests that whey biocomposite films, when strengthened with various additives, have the potential to be used as edible food packaging materials with desirable attributes. However, despite extensive studies, the utilization of WBF in model food systems remains limited, highlighting a significant gap for further exploration. Further research in this domain could potentially unlock new opportunities for utilizing WBF in various model food systems. [ABSTRACT FROM AUTHOR]