1. Confirmation of five nitrofuran metabolites including nifursol metabolite in meat and aquaculture products by liquid chromatography-tandem mass spectrometry: Validation according to European Union Decision 2002/657/EC
- Author
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Michel Laurentie, Eric Verdon, Pierre Guichard, Dominique Hurtaud-Pessel, Laboratoire de Fougères - ANSES, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), and This work was financially supported by the European Commission Directorate-General for Health and Food Safety (European contribution to the European Union Reference Laboratory SI2.801891).
- Subjects
MESH: Nitrofurans ,Nitrofurans ,Metabolite ,Aquaculture ,01 natural sciences ,Analytical Chemistry ,Matrix (chemical analysis) ,chemistry.chemical_compound ,Liquid chromatography–mass spectrometry ,Limit of Detection ,Tandem Mass Spectrometry ,Nitrofurane ,Sample preparation ,Nitrofuran ,Chromatography, High Pressure Liquid ,media_common ,MESH: Meat ,Semicarbazide ,Muscles ,04 agricultural and veterinary sciences ,General Medicine ,Residues ,Reference Standards ,040401 food science ,Nifursol ,Chromatographie liquide ,Meat ,Liquid chromatography/mass spectrometry ,medicine.drug_class ,Métabolite ,Viande ,Spectrométrie de masse ,0404 agricultural biotechnology ,[CHIM.ANAL]Chemical Sciences/Analytical chemistry ,medicine ,media_common.cataloged_instance ,Animals ,European union ,LC-MS/MS ,MESH: Chromatography, High Pressure Liquid ,Detection limit ,Chromatography ,010401 analytical chemistry ,MESH: Tandem Mass Spectrometry ,Reproducibility of Results ,Médicament vétérinaire ,0104 chemical sciences ,chemistry ,Veterinary drug ,Analysis ,Food Science - Abstract
International audience; LC-MS/MS method for confirmation of nitrofuran metabolites in meat and aquaculture products, including the nifursol metabolite (DNSH), was developed. The nitrofuran metabolites investigated were as follows: 3-amino-2-oxazolidinone (AOZ), 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ), 1-aminohydantoine (AHD), semicarbazide (SEM) and 3,5-dinitrosalicylic acid hydrazide (DNSH). The sample preparation includes a washing step, allowing to analyze only the fraction of protein-bound residues. The final optimized recovery solvent for injection was found to be a mixture of ammonium acetate 2 mM/acetonitrile (60/40 ; v/v). Matrix effects and stability in biological matrix and standard solution for DNSH have been also carried out. Method performances were assessed using criteria of the Decision (EC) No 2002/657 and considering the proposed-for-adoption reference point for action (RPA) of 0.50 µg.kg−1 under Reg 1871/2019. Trueness ranged 86.5%–103.7% and precision ranged 2.0%–6.5% on intra-laboratory reproducibility conditions. Decision limit (CCα) ranged 0.028–0.182 μg.kg−1 and capability of detection limit (CCβ) ranged 0.032–0.233 µg.kg−1.
- Published
- 2020