1. Rapid measurement of phytosterols in fortified food using gas chromatography with flame ionization detection
- Author
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Katherine Stockham, Bill Wales, Samantha Duong, Norbert Strobel, Saman Buddhadasa, Marlene J. Cran, Martin J. Auldist, and John D. Orbell
- Subjects
0301 basic medicine ,Accuracy and precision ,Meat ,Cost effectiveness ,Chemical Fractionation ,01 natural sciences ,Analytical Chemistry ,law.invention ,03 medical and health sciences ,law ,Animals ,Plant Oils ,Flame ionization detector ,Fortified Food ,Flame Ionization ,030109 nutrition & dietetics ,Chromatography ,Chemistry ,Phytosterol ,010401 analytical chemistry ,Phytosterols ,General Medicine ,0104 chemical sciences ,Milk ,Food, Fortified ,Gas chromatography ,Gas chromatography–mass spectrometry ,Saponification ,Food Science - Abstract
A novel method for the measurement of total phytosterols in fortified food was developed and tested using gas chromatography with flame ionization detection. Unlike existing methods, this technique is capable of simultaneously extracting sterols during saponification thus significantly reducing extraction time and cost. The rapid method is suitable for sterol determination in a range of complex fortified foods including milk, cheese, fat spreads, oils and meat. The main enhancements of this new method include accuracy and precision, robustness, cost effectiveness and labour/time efficiencies. To achieve these advantages, quantification and the critical aspects of saponification were investigated and optimised. The final method demonstrated spiked recoveries in multiple matrices at 85-110% with a relative standard deviation of 1.9% and measurement uncertainty value of 10%.
- Published
- 2016