1. Characterizing gangliosides in six sea cucumber species by HILIC-ESI-MS/MS.
- Author
-
Wang X, Wang X, Cong P, Zhang X, Zhang H, Xue C, and Xu J
- Subjects
- Animals, Gangliosides chemistry, Glucosinolates chemistry, N-Acetylneuraminic Acid chemistry, Gangliosides analysis, Sea Cucumbers chemistry, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry
- Abstract
An HILIC-ESI-MS/MS method was established to analyze ganglioside (GLS) in sea cucumbers. In total, 17 GLS subclasses were detected in six sea cucumber species. The basic sea cucumber GLSs (SC-GLSs) were elucidated as NeuGc2-6Glc1-1Cer (SC-GM
4 ). The polymerization degree of the sialic acid (Sia) of SC-GLSs can be up to 4, and the linkage among Sias was mostly determined to be 2-8 or 2-11. Neu5Gc, sulfated and fucosylated NeuGc prevalently existed in SC-GLSs. Moreover, a new SC-GLSs structure with phosphoinositidyled Sia was first observed in Bohadschia marmorata. For the first time, we demonstrated that the content of SC-GD4 , which is the dominant GLS in sea cucumbers, was 27-67%. Minor GLSs characterized as SC-GT2 (Neu5GcMe) and SC-GQ2 (Neu5GcMe) were also discovered. Additionally, SC-GD4 and SC-GD4 (1S) could significantly promote the differentiation of PC12 cells with structure-selectivity (p < 0.05). Our results provide insights into SC-GLSs to elucidate their Sia substituent and core saccharide chain linkage., (Copyright © 2021 Elsevier Ltd. All rights reserved.)- Published
- 2021
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