1. New neolignans from the seeds of Myristica fragrans that inhibit nitric oxide production.
- Author
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Cao, Gui-Yun, Xu, Wei, Yang, Xiu-Wei, Gonzalez, Frank J., and Li, Fei
- Subjects
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NEOLIGNANS , *COMPOSITION of seeds , *NITRIC-oxide synthase inhibitors , *LIPOPOLYSACCHARIDES , *POLYMERASE chain reaction , *MESSENGER RNA , *NUTMEG tree - Abstract
Five new 8- O -4′ type neolignans, named myrifralignan A–E ( 1 – 5 ), together with five known analogues ( 6 – 10 ), were isolated from the seeds of Myristica fragrans Houtt. Their chemical structures were determined using several spectroscopic methods. Compounds 3 – 10 exhibited potent inhibitory activity against the production of nitric oxide (NO) in the RAW264.7 cell line stimulated by lipopolysaccaride. Myrislignan ( 7 ) and machilin D ( 10 ) were the most potent inhibitors of NO production amongst these compounds. The IC 50 values of myrislignan and machilin D were 21.2 and 18.5 μM. And, their inhibitory activity was more than L - N 6 -(1-iminoethyl)-lysine, a selective inhibitor of inducible nitric oxide synthase (IC 50 = 27.1 μM). Furthermore, real-time PCR analysis revealed that these neolignans could significantly suppress the expression of inducible nitric oxide synthase mRNA. These results demonstrated that the 8- O -4′ type neolignans are promising candidates as anti-inflammatory agents. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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