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A liquid chromatographic method is presented for the determination of the phenylurea herbicide diuron and its major metabolite, 3,4-dichloroaniline in asparagus. The method involves a simple isolation step using dichloromethane extraction, followed by reversed phase chromatography on a C18 column, using a basic aqueous-organic eluent and UV detection at 254 nm. The limit of determination is 0.02 mg/kg for both analytes.