1. Evaluation of the antifungal activity of α, β, and δ-damascone and inclusion complexes in β-cyclodextrin against Candida spp.
- Author
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de Oliveira MRC, de Lima Silva MG, Dos Santos ATL, Batista FLA, da Costa RHS, Martins AOBPB, da Cruz BG, Morais-Braga MFB, Coutinho HDM, Magalhães FEA, de Sena Junior DM, Teixeira AMR, and de Menezes IRA
- Subjects
- Candida, Fluconazole pharmacology, Microbial Sensitivity Tests, Norisoprenoids pharmacology, Antifungal Agents chemistry, Antifungal Agents pharmacology, beta-Cyclodextrins pharmacology
- Abstract
Due to the increase in fungal resistance to existing drugs, a need exists to search for new antifungals. This study aimed to evaluate the antifungal activity of α, β, and δ-damascone and inclusion complexes with β-cyclodextrin against different Candida spp. The inclusion complex of β-damascone was prepared by the co-evaporation method using three molar proportions (1:1; 2:1; 3:1 (βDA-βCD)) and analyzed using Fourier transform infrared spectroscopy (FTIR). Standard Candida albicans (CA INCQS 40,006), Candida krusei (CK INCQS 40,095), and Candida tropicalis (CT INCQS 40,042) strains were used to evaluate antifungal activity. The substances were tested individually or in association with fluconazole (FCZ). The IC
50 and cell viability curve constructions were performed using the microdilution method. The minimum fungicidal concentration (MFC) was determined by the subculture method in a solid medium. The α, β, and δ-DA isolated or in combination with fluconazole (FCZ) showed significant antifungal activity. β-damascone showed effective complexation in the three molar proportions assayed; however, none of the inclusion complexes was demonstrated clinically significant effects against the fungal tested. Then, all compounds have shown promising antifungal activities; however, in vivo assays are necessary to have therapeutical application in the future., (© 2022. Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i.)- Published
- 2022
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