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3 results on '"Cuesta Rubio, O."'

1. Chemical profile and anti-leishmanial activity of three Ecuadorian propolis samples from Quito, Guayaquil and Cotacachi regions

Author

Mercedes Campo Fernández, David Marrero Delange, Ingrid Márquez Hernández, Luca Rastrelli, Carmita Gladys Jaramillo Jaramillo, Rodny Montes de Oca Porto, Osmany Cuesta-Rubio, Anna Lisa Piccinelli, Luca Campone, Lianet Monzote Fidalgo, Victor Hugo González, Cuesta-Rubio, O, Campo Fernandez, M, Marquez Hernandez, I, Jaramillo, C, Gonzalez, V, Montes De Oca Porto, R, Marrero Delange, D, Monzote Fidalgo, L, Piccinelli, A, Campone, L, and Rastrelli, L

Subjects
Ecuadorian propolis, Triterpenoid, Cells, Antiprotozoal Agents, Antileishmanial activity, Flavonoids, GC–MS, NMR, Animals, Cells, Cultured, Ecuador, Leishmania, Macrophages, Mice, Inbred BALB C, Propolis, Triterpenes, Pharmacology, Drug Discovery3003 Pharmaceutical Science, 01 natural sciences, Terpene, Ecuadorian propoli, Mice, Drug Discovery, GC–MS, Anti leishmanial, Inbred BALB C, GCâ MS, Leishmania amazonensis, Cultured, biology, Traditional medicine, 010405 organic chemistry, Chemistry, General Medicine, biology.organism_classification, Nmr data, 0104 chemical sciences, 010404 medicinal & biomolecular chemistry, Flavonoid, Gas chromatography–mass spectrometry
Abstract

Three propolis samples were collected from different regions of Ecuador (Quito, Guayaquil and Cotacachi) and their methanolic extracts were prepared. Preliminary information supplied by TLC and NMR data, allowed us to define two main types of propolis: Cotacachi propoli sample (CPS), rich in flavonoids and Quito and Guayaquil samples (QPS and GPS) containing triterpenic alcohols and acetyl triterpenes as the main constituents. Two different approaches based on RP-HPLC preparative procedure and NMR structural determination (CPS) and GC–MS analysis (QPS and GPS) were successfully used for the chemical characterization of their major compounds. All three propolis extracts were able to inhibit Leishmania amazonensis growth but propolis sample rich in flavonoids was the most active (IC50 = 17.1 ± 1.7 μg/mL). In the literature this is the first study on propolis from Ecuador.

Published
2017

2. Chemosensitizing activity of Cuban propolis and nemorosone in doxorubicin resistant human colon carcinoma cells.

Author

Frión-Herrera Y, Gabbia D, Díaz-García A, Cuesta-Rubio O, and Carrara M

Subjects
Apoptosis drug effects, Cell Cycle Checkpoints drug effects, Cell Line, Tumor, Cell Proliferation drug effects, Colonic Neoplasms, Cuba, Humans, Membrane Potential, Mitochondrial drug effects, Reactive Oxygen Species metabolism, Antineoplastic Agents pharmacology, Benzophenones pharmacology, Doxorubicin pharmacology, Drug Resistance, Neoplasm, Propolis pharmacology
Abstract

Propolis is a natural product obtained from bees, used since ancient times for its multiple pharmacological properties. Several evidences indicate that the antiproliferative effect of propolis against different cancer cell lines can be ascribed to its components. However, little is known about the possible use of this natural product in the treatment of chemo-resistant tumors. Combination experiments were carried out in order to study the ability of Cuban propolis extracts (CP) and its main component (nemorosone) to chemosensitize doxorubicin-resistant human colon carcinoma cells (LoVo Dox) compared to the sensitive cells (LoVo). Antiproliferative effect was determined by MTT assay after 24, 48 and 72 h exposure. Synergistic, additive or antagonistic effects of different combined treatments (CP-Dox and nemorosone-Dox), was evaluated by isobologram-combination index method. The interaction mechanisms between CP or nemorosone with doxorubicin were studied by flow cytometry to investigate cell death pathway and cell cycle arrest. Reactive oxygen species production (ROS) and mitochondrial membrane potential (ΔΨm) modification were also evaluated. Data showed that both CP and its main component nemorosone were able to reduce cell proliferation in a concentration- and time-dependent manner. Combined treatments induced a cell growth inhibition with a significantly synergistic antiproliferative and cytotoxic effect. Co-treatments induced also cell cycle arrest which results in apoptosis by a marked ROS production and drastic alteration of ΔΨm. In summary, our findings evidence the potential role of Cuban propolis extracts and their main component nemorosone as new chemosensitizing agents against drug-resistant human colon carcinoma cells., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Published
2019
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3. Chemical profile and anti-leishmanial activity of three Ecuadorian propolis samples from Quito, Guayaquil and Cotacachi regions.

Author

Cuesta-Rubio O, Campo Fernández M, Márquez Hernández I, Jaramillo CGJ, González VH, Montes De Oca Porto R, Marrero Delange D, Monzote Fidalgo L, Piccinelli AL, Campone L, and Rastrelli L

Subjects
Animals, Antiprotozoal Agents isolation & purification, Cells, Cultured, Ecuador, Flavonoids isolation & purification, Leishmania drug effects, Macrophages parasitology, Mice, Inbred BALB C, Triterpenes isolation & purification, Antiprotozoal Agents chemistry, Flavonoids chemistry, Propolis chemistry, Triterpenes chemistry
Abstract

Three propolis samples were collected from different regions of Ecuador (Quito, Guayaquil and Cotacachi) and their methanolic extracts were prepared. Preliminary information supplied by TLC and NMR data, allowed us to define two main types of propolis: Cotacachi propoli sample (CPS), rich in flavonoids and Quito and Guayaquil samples (QPS and GPS) containing triterpenic alcohols and acetyl triterpenes as the main constituents. Two different approaches based on RP-HPLC preparative procedure and NMR structural determination (CPS) and GC-MS analysis (QPS and GPS) were successfully used for the chemical characterization of their major compounds. All three propolis extracts were able to inhibit Leishmania amazonensis growth but propolis sample rich in flavonoids was the most active (IC 50 =17.1±1.7μg/mL). In the literature this is the first study on propolis from Ecuador., (Copyright © 2017. Published by Elsevier B.V.)

Published
2017
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