1. Effects of Moritella viscosa antigens on pro-inflammatory gene expression in an Atlantic salmon (Salmo salar Linnaeus) cell line (SHK-1)
- Author
-
Laura L. Brown, Sandra Sperker, B K Gudmundsdóttir, Mark D. Fast, and Bryndis Bjornsdottir
- Subjects
MvP1 vibriolysin ,Atlantic salmon ,Cell Survival ,Interleukin-1beta ,Salmo salar ,Gene Expression ,Moritella viscosa ,Aquatic Science ,Microbiology ,Cell Line ,Fish Diseases ,Immune system ,Antigen ,Gene expression ,Extracellular ,Environmental Chemistry ,Cytotoxic T cell ,Animals ,Interleukin 8 ,RNA, Messenger ,Immune response ,Salmo ,Antigens, Bacterial ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Macrophages ,Interleukin-8 ,Metalloendopeptidases ,General Medicine ,Extracellular products ,Pro-inflammatory cytokine ,biology.organism_classification ,SHK-1 ,Interleukin-8 (IL-8) ,Cell culture ,Vibrio Infections ,Immunology ,Interleukin-1β (IL-1β) ,Moritella - Abstract
Moritella viscosa is the causative agent of winter ulcer disease in salmonids reared in North-Atlantic countries. In this study the effects of selected M. viscosa antigens on cytotoxicity and pro-inflammatory gene expression in an Atlantic salmon (Salmo salar Linnaeus) macrophage-like cell line (SHK-1) were examined. SHK-1 cells were stimulated with live and heat-killed bacterial cells, extracellular products (ECP) and an extracellular vibriolysin, termed MvP1. Following incubation, cytotoxicity and expression levels of interleukin-1 beta (IL-1 beta) and interleukin-8 (IL-8) were examined at different time points. Both live M. viscosa cells and ECP were cytotoxic, but neither heat-killed cells, nor the MvP1 peptidase caused cell death. Expression levels of both IL-1 beta and IL-8 increased significantly after stimulation with live cells, but heat-killed cells only caused increased IL-8 expression. ECP did not affect IL-1 beta expression, but did stimulate IL-8 expression. The isolated MvP1 peptidase stimulated both IL-1 beta and IL-8 expression at the highest concentration tested. This study reveals a difference in the induction of pro-inflammatory gene expression in salmon SHK-1 cells between live and heat-killed M. viscosa cells, and also that an unknown secreted factor is the main stimulant of IL-beta and IL-8 expression.
- Published
- 2009