1. Characterization of a lectin from the craysfish Cherax quadricarinatus hemolymph and its effect on hemocytes.
- Author
-
Sánchez-Salgado JL, Pereyra MA, Vivanco-Rojas O, Sierra-Castillo C, Alpuche-Osorno JJ, Zenteno E, and Agundis C
- Subjects
- Agglutination Tests, Amino Acids analysis, Animals, Blotting, Western, Chromatography, Affinity, Electrophoresis, Gel, Two-Dimensional, Hemocytes metabolism, Hemolymph metabolism, Immunohistochemistry, Lectins blood, Lectins immunology, Mass Spectrometry, Phagocytosis immunology, Reactive Oxygen Species metabolism, Respiratory Burst immunology, Statistics, Nonparametric, Astacoidea chemistry, Astacoidea immunology, Hemocytes immunology, Lectins analysis
- Abstract
Lectins participate in the immune mechanisms of crustaceans. They have been considered as humoral receptors for pathogen-associated molecular patterns; however, some reports suggest that lectins could regulate crustacean cellular functions. In the present study, we purified and characterized a serum lectin (CqL) from the hemolymph of Cherax quadricarinatus by affinity chromatography and determined its participation in the regulation of hemocytes' oxidative burst. CqL is a 290-kDa lectin in native form, constituted by 108, 80, and 29-kDa subunits. It is mainly composed of glycine, alanine, and a minor proportion of methionine and histidine. It showed no carbohydrates in its structure. CqL is composed of several isoforms, as determined by 2D-electrophoresis, and shows no homology with any crustacean protein as determined by Lc/Ms mass spectrometry. CqL agglutinated mainly rat and rabbit erythrocytes and showed a broad specificity for monosaccharides such as galactose, glucose, and sialic acid, as well as for glycoproteins, such as porcine stomach and bovine submaxillary mucin and fetuin. It is a Mn(2+)-dependent lectin. CqL recognized 8% of crayfish granular hemocytes and increased 4.2-fold the production of hemocytes' superoxide anion in vitro assays when compared with non-treated hemocytes. This effect showed the same specificity for carbohydrates as hemagglutination; moreover, superoxide dismutase and diphenyleneiodonium chloride were effective inhibitors of CqL oxidative-activation. The CqL homoreceptor is a 120-kDa glycoprotein identified in the hemocytes lysate. Our results suggest that CqL participates actively in the regulation of the generation of superoxide anions in hemocytes using NADPH-dependent mechanisms., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF