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Late foliar diseases that appear during the grain filling period reduce radiation interception/absorption decreasing assimilation supply, inducing reductions in grain weight and yield. We hypothesize that crops with higher source-sink ratio (established immediately after anthesis) could have more capacity to avoid reductions in terms of yield, at same level of foliar disease. Thus, it is speculated that the yield reduction caused by late foliar diseases is a complex interaction between current photosynthesis availability per grain and the remobilization capacity according to the sink size determined demand. The objective of the present study was to analyze physiological attributes associated with yield reduction caused by late foliar diseases in wheat when source-sink ratio is modified. Experiments were carried out during two consecutive years (2009 and 2010) applying a combination of (i) two disease levels (i.e. healthy and diseased plots), (ii) two different levels of incident radiation applied immediately previous to anthesis, to reduce grain number exclusively (i.e. non-shaded and shaded plots) and (iii) source-sink manipulations during grain filling (i.e. control and trimmed spikes). Shading during pre-anthesis significantly reduced grain number and then, the source-sink ratio was modified between non-shaded and shaded plots, being two fold higher in non-shaded than in shaded plots. In both years and shading treatments, diseases appeared after anthesis, the diseased plots showing higher values of non-green leaf area than healthy plots, in both shaded and non-shaded treatments. Late foliar diseases significantly reduced grain yield in both, 2009 and 2010 mainly due to reductions in grain weight. There was significant interaction in the crop performance to foliar diseases between shading treatments and growing seasons. While the reduction caused by late foliar diseases was similar between shading treatments during 2009, in 2010 significant differences were observed, being reductions lower in shaded than in non-shaded plots. The source-sink manipulation treatment confirmed these responses, as there were no significant differences in grain weight of trimmed spikes between healthy and diseased crops, confirming that the grain weight reduction due to late foliar diseases is driven by the source sink balance.

Promising yield-determining responses to artificial photoperiod manipulation during post-flowering phases have been identified in soybean [Glycine max (L.) Merrill]. These responses enhance resource capture as a result of changes in phase duration, and resource use efficiency as a result of changes in node production and pod development, alleviating intra-node pod interference. This work aims to evaluate the relevance of these responses under natural conditions and discriminate direct from indirect photoperiodic effects, that is, effects caused by changes in the capture of resources. Two field experiments were conducted with a combination of sowing dates, with similar pre- and different post-flowering photoperiod, indeterminate genotypes including near isogenic lines and commercial varieties with different photoperiod sensitivity, and shadings with different incident radiation during post-flowering. Longer post-flowering photoperiod and higher plant photoperiod sensitivity delayed crop development, particularly in lines with alleles E2, E3 or Clark background. This delay resulted in extra radiation capture and node production, which were positively related to yield. Direct photoperiodic effects on nodes per m2 were evidenced in response to changes in post-flowering photoperiod and plant photoperiod sensitivity. The distribution of available resources such as radiation in more nodes per m2 increased use efficiency. Longer post-flowering photoperiod and higher plant photoperiod sensitivity enhanced pod establishment on usually dominated positions such as lateral racemes, affecting pods per m2. Finding these relevant yield-determining effects under natural conditions suggests that processes related to post-flowering photoperiodic responses are a plausible way to enhance resource capture and use efficiency and yield in soybean production systems. In cropping systems in which the whole growing season is used to maximize yield, these post-flowering photoperiodic responses could be adopted at the expense of the pre-flowering phase duration.

Malt extract is the most relevant parameter describing malting quality in barley. The observed differences in malt extract from crops grown in different environments are complex to explain, and their interpretation might represent an opportunity to improve malting quality for barley crops grown under field conditions. Although the effect of some grain attributes on malt extract are overall known (e.g. protein content and grain size) other are less understood and are complex to replicate across experiments, with few evidence of how they are controlled by the environment during grain filling (e.g. hordeins and pasting properties). Four commercial malting barley cultivars were sown on eleven sowing dates across four years to explore a wide range of thermal conditions during grain filling. Contrasting nitrogen availabilities were included to promote variability in the protein content. Grain plumpness, protein content, pasting temperature, and the amount of d - and γ-hordeins showed a significant effect on malt extract. Protein content showed a negative effect on malt extract only when it was above a threshold value of ca. 10 %, although that threshold varied between cultivars. Pasting temperature presented a significant interaction with protein content. d -hordein showed a negative effect on malt extract only when the protein content was below the threshold, explaining some of the contradictory results reported in the literature. Evidence of a negative effect of γ-hordeins on malt extract was found as well. The period between pollination and physiological maturity was arbitrarily divided into thermal-time intervals, and correlation analyses were performed between mean temperature during each period and the grain attributes affecting malt extract. For all the cultivars, only two attributes were found to be modulated by the thermal environment explored by the crop during grain filling: pasting temperature was positively correlated to the mean temperature explored by the crop during the middle of grain filling, whereas grain plumpness was negatively correlated with the mean temperature during the second half of grain filling. Quantitative models reported in the present study in addition to offering an explanation of how malting quality is shaped in the field, constitute a useful tool for agro-climatic zoning for the suitability of high-quality malting barley production.

Sunlight is a crucial environmental factor for photosynthesis. Plant density affects both quality and quantity at light penetration into the canopy. The effects of plant density on the expression of photomorphogenic traits in cultivated spring rapeseed (Brassica napus L.), and their consequences on seed production per unit area are unknown. The aims of this work were to: i) analyze the magnitude of shade avoidance responses to plant densities in five spring rapeseed genotypes, ii) describe the dynamics of floral branching in response to genotype and plant density, and iii) study yield and its components and seed quality in field-cultivated plants with contrasting plant densities (15 and 240 pl m−2). Rosette diameter was the main attribute of vegetative plasticity modified by plant density, without significant changes in petiole length. Plant density changed the relationship between intercepted solar radiation (ISR) and red/far red ratio (R/FR) during crop development, but at flowering all densities reached ISR = 95% and R/FR ratio

tThe use of physiological traits for indirect selection may help to increase wheat yield potential. Fruitingefficiency (FE, grain number per unit of spike dry weight at anthesis −SDWa-) may be an alternativeto increase grain number per unit area (GN), but the possible cross-over genotype (G) × environment(E) interaction and trade-off with SDWa and grain weight (GW) may limit its usefulness. The presentpaper aimed to determine (i) the degree of G × E interaction of FE at plot, main stem and tiller levels,(ii) the relationship between GN and FE, and (iii) the possible trade-off between FE and (a) SDWa, and(b) GW. Three FE contrasting genotypes (B11 and B19 ? high FE, and B2002 ? low FE) were grown underdifferent Nitrogen (N) availability at sowing [(i) low ? Ni: ca. 50 kg N ha−1; (ii) middle −130 kg N ha−1and (iii) high −200 kg N ha−1). Two other treatments were applied within the 200N: (iv) late sowing, i.e.delaying sowing 30 days in relation to optimum date (200N + LS) and (v) shading the canopy during stemelongation (200N + SH). The FE showed G × E interaction (year × genotype and genotype × treatment) butit only explained 13.4% of its variation. The environment (year) had similar impact than the genotype,explaining both ca. 38% of FE variation (19% each). The FE of tiller spikes was higher than that of mainstem spikes, but the difference among cultivars was maintained. Thus, canopies of the same genotypewith different proportion of main stem and tiller spikes may present different FE value at plot level.Nevertheless, a high correlation (r = 0.82, p < 0.0001) and a close 1:1 relationship was observed between FEat plot level and FE in main stems, for all treatments and cultivars, suggesting that sampling the main stemspikes represents the FE at plot level in dense canopies. A G × E cross-over interaction was observed whenthe sowing was delayed one month (200N + LS) from the optimum date because B19 reached anthesislater than B2002, exploring higher heat stress index (◦Ch > 27◦C) around anthesis, which reduced FE.The correlation of GN with FE was high and significant (r = 0.74, p < 0.00001) for all treatments and fora wide range of SDWa values (from 90 to 188 g m−2). The B19, selected as a high FE genotype, showedhigher GN than B2002 (selected as a low FE genotype) under all treatments, while B11 (also of high FE)showed similar or higher GN than B2002, depending on treatment. Thus, despite the G × E interaction, thegenotypes with higher FE had more GN under a wide range of environments. Nevertheless, the SDWa andGW were negatively correlated with FE (for SDWa r = −0.73 p < 0.005, without considering 200N + SH; andfor GW r = −0.48 p < 0.01 for all treatments or r = −0.75 p < 0.08 for genotypes only). Then, special attentionshould be given to SDWa and GW when selecting for higher FE in a breeding program. Fil: Terrile, Ignacio Ismael. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Norte. Estación Experimental Agropecuaria Pergamino; Argentina Fil: Miralles, Daniel Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Cerealicultura; Argentina Fil: González, Fernanda Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Norte. Estación Experimental Agropecuaria Pergamino; Argentina

Tan spot [Pyrenophora tritici-repentis (Died.) Drechs., anamorph Drechslera tritici-repentis) (Died.) Shoem.] and leaf rust (Puccinia triticina Eriks) are major diseases worldwide and some of the main biotic causes of yield and quality reduction in wheat (Triticum aestivum L.). Although wheat crop losses due to foliar diseases have already been studied based on an ecophysiological approach, none of these studies analyzed the independent effects of foliar pathogens with different nutritional habits. The aim of the present study was to determine the independent effects of (i) Py. tritici-repentis (necrothroph) and (ii) Puccinia triticina (biotroph) on the physiological components of biomass production: accumulated intercepted by green tissue photosynthetic active radiation (IGPAR), radiation use efficiency calculated by intercepted radiation (RUEint) as well as by intercepted by green tissue (RUEgt) in a wide range of Argentine commercial bread wheat cultivars growing in two field environments. Field experiments were carried out during two consecutive years combining a large range of wheat bread commercial cultivars and two levels of inoculation to promote infection of Tan spot and Leaf rust diseases, including a control without inoculation. Treatments were arranged in an experimental split-split plot design with three replications, where the main plots were both diseases, subplots corresponded to inoculation treatments 1- without inoculation (WI), 2- low concentration of inoculum of each disease (LC), 3- high concentration of inoculum of each disease (HC) and ten Argentine bread wheat commercial cultivars were the sub-subplots. Area under disease progress (AUDPC), area under percentage of non-green leaf area (AU %NGLA), crop growth rate (CGR) and healthy area duration (HAD) were calculated. Green leaf area index (GLAI), aboveground biomass (AGB), IGPAR and RUE were measured at three different crop stages (GS39, GS61 and GS82). Increases of inoculum concentration decreased AGB between 8 and 20% mainly explained by reductions in HAD and decreases on IGPAR by 14–18% with higher reductions when the crop was inoculated with Py. tritici-repentis than with P. triticina. Although both diseases reduced the physiological components of accumulated biomass related to radiation interception (IGPAR), RUEint (−23%), RUEgt (−11%) and CGR (−29%) were more reduced, respect to WI, when plants were inoculated with P. triticina compared to Pyrenophora tritici-repentis. The differential responses in RUE could be associated with the nutritional habit of P. triticina that reduces leaf nitrogen concentration, enhance assimilates consume by leaf respiration, reducing radiation use efficiency. Fil: Schierenbeck, Matías. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fleitas, María Constanza. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Miralles, Daniel Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina Fil: Simon, Maria Rosa. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

In wheat, warmer nights during the critical period reduce grain yield due to a diminished grain number per unit area, associated with lower spike number. Previous studies have evaluated the impact of increased night temperatures, but only under potential growing conditions. The aims of this study were to: (i) evaluate the impact of warmer nights during the critical period (i.e. from third detectable node to 10 days after anthesis) on grain yield and its numerical components, under contrasting nitrogen (N) and water levels and (ii) determine whether plant density could be useful as a management strategy to reduce yield losses caused by warmer nights. Two sets of experiments were conducted in Buenos Aires, the first in 2015 and 2016. In both years trials were carried out combining (i) two levels of nitrogen availability (60 and 200 kg N ha−1), (ii) two levels of water availability (rainfed and irrigated) and (iii) two conditions of night temperature (increased night temperature from third visible node to 10 days after anthesis and unheated crops during the whole crop cycle). The second experiment was performed in 2017 and combined: (i) three plant densities (ca. 100, ca. 150 and 300 plants m−2) and (ii) two-night temperature regimes (increased night temperature from third visible node to 10 days after anthesis and unheated crops during the whole crop cycle). Night temperatures were increased by heating systems, during the critical period average night temperatures ranged from 10.7 °C to 15.1 °C. Yield losses occurred when plants were exposed to warmer nights at high nitrogen levels (ca. 7% - i.e. 550 g m−2 - loss per °C of increase) but no effects were apparent under lower nitrogen availability. Warmer nights increased tiller mortality only under high nitrogen availability. No conclusions could be reached concerning water supply management or the convenience of this strategy to reduce the impact of warmer nights due to the lack of response of grain yield or grain number to modifications in water availability. Grain yield was significantly reduced by increases in night temperature only under higher nitrogen supply mostly due to a reduction in grain number per unit area, associated with reductions in spike dry weight; however, crops under high nitrogen levels yielded significantly more than those grown under low nitrogen, even when exposed to warmer nights. For all three densities, warmer nights reduced grain yield similarly (on average ca. 15 %).

Precise phenology modelling of grain filling is essential to understand how the environment explored by barley crops in the field affects malting quality. Grain-filling duration is controlled by temperature; hence thermal time models are widely used for describing grain development. Although a precise base temperature is essential for the accuracy of thermal time models, estimations of this parameter for barley grain filling are scarce in the literature; moreover, the methods employed for its determination seldom establish clearly whether grain filling is being treated as a development or as a growth phenomenon. Even worse, the base temperature during grain filling is usually arbitrarily set to zero. In this work we tested five different methods to estimate the thermal time requirement for completing grain filling in four commercial barley cultivars and the base temperature above which temperature must be accumulated for its calculation, using field-collected data. The crops were sown in a wide range of different dates during three consecutive years and grown under two contrasting nitrogen availabilities. All the estimation methods performed relatively well. Developmental base temperature was close to 8 °C for all cultivars, although genotypic differences were noted. Optimum temperatures for grain filling are reported for three of the four cultivars; however, including this parameter in the models only slightly improved the predictions in one out of three cultivars. The effect of nitrogen availability was negligible and inconsistent across cultivars and environments thus indicating that the models developed can be applied in a wide range of nutritional situations. A broad application model, with a base temperature of 8.4 °C and a thermal time requirement for completing grain filling of 352 °Cd, is reported for barley grain development. All the models were validated against independent data. These models can be used by researchers aiming to unscramble the effects of environment explored by the mother plant on grain malting quality. Further applications include predicting the timing of physiological maturity, and with it, harvest time.

Wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) crops are exposed to warm nights during their growing seasons and this trend is unlikely to change. The aim of this work was to evaluate the effect of higher post-anthesis night temperatures on field-grown crop yield, focusing on final grain weight determination. Experiments combined: (i) two well-adapted crops with similar phenology: bread wheat and two-row malting barley, under (ii) two temperature regimes: ambient and high night temperatures from 10 days after anthesis to physiological maturity during (iii) two contrasting growing seasons in terms of radiation and temperature: late sowing in 2011 and early sowing in 2013. The night temperature increase (ca. 4.1 °C) was achieved using purpose-built heating chambers placed on the crop at 7 pm and removed at 7 am every day during the heating period. Across growing seasons and crops, the average minimum temperature during that period ranged from 14.3 °C to 21.9 °C. Thousand grain weight was reduced by ca. 3% per °C of night temperature increase, similarly for wheat and barley, causing a grain yield reduction of ca. 4% per °C. An accelerated development under high night temperatures led to a shorter effective grain filling period, reducing the final grain weight. The lack of consistent impact on source availability between crops and seasons, measured as senescence and stem water soluble carbohydrates, as well as a similar impact in magnitude and direction on individual grain weight for different grain positions along wheat or barley spikes, suggest that the negative effects of warm nights on grain weight were directly related to processes within the grain itself. Fil: García, Guillermo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina Fil: Serrago, Roman Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina Fil: Dreccer, M. Fernanda. The University Of Queensland; Australia Fil: Miralles, Daniel Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina

Predicting phenology, in particular heading time, is crucial to avoid and/or minimize risk of damage offrost, drought and high temperatures during grain filling. Although some of the major genes controllingdevelopment, associated with photoperiod and vernalization responses, were identified, the associationbetween the molecular characterization of those genes and photoperiod sensitivity, vernalizationresponses and earliness per se has been poorly studied. The present study was conducted to determinethe effects of photoperiod and vernalization genes (and their allelic combination characterized by molecularapproach) on heading time and its correlation with the phenological parameters determined by fieldexperiments in a wide range of Argentine bread wheat commercial cultivars. Additionally, the associationbetween photoperiod and vernalization responses with earliness per se was analyzed. Molecularcharacterization showed that most of the commercial Argentine wheat cultivars available in the marketcorrespond to spring growth habit with dominant insensitive photoperiod alleles (SI) followed by springhabit sensitive to photoperiod (SS), while winter insensitive (WI) habit represented a minority group. Allgenotypes included in the present study (even those classified as SI and WI) were photoperiod sensitivewhen that trait was quantified from a physiological analysis as the slope of the relationship betweenduration of a particular phase and mean photoperiod sensed during the period between emergence andheading. SI showed lower photoperiod sensitivity than SS and WI, without clear differences betweenboth later groups. In all cases, photoperiod sensitivity was the main attribute that determined the differencesin time to heading even when vernalization requirements were not completely fulfilled in the WI.The genotypes with different photoperiod and vernalization allele combinations showed a wide range ofduration of earliness per se. However, differences in earliness per se did not show any particular associationwith the groups classified by molecular markers for photoperiod and vernalization. The informationincluded in the present study can be used to build a gene-based model for predicting phenology. However,the variations in photoperiod and/or vernalization sensitivity within the same allelic combinationcould still determine mismatching in the prediction of the models based on Ppd-1 and Vrn-1 genes. Fil: Gomez, Dionisio Tomas. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuario Marcos Juarez; Argentina Fil: Vanzetti, Leonardo Sebastián. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuario Marcos Juarez; Argentina Fil: Helguera, Marcelo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuario Marcos Juarez; Argentina Fil: Lombardo, Lucio Andrés. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuario Marcos Juarez; Argentina Fil: Fraschina, Jimena. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuario Marcos Juarez; Argentina Fil: Miralles, Daniel Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Cerealicultura; Argentina

In wheat, stem elongation phase (SEP) duration is critical for grain number (GN) per unit of area determination, as it is the phase in which the spikes grow. Lengthening SEP, for instance by photoperiodic sensitivity, without altering the cycle to anthesis (AT) has been proposed as an alternative way to increase spike dry weight, and in turn GN. As most works supporting this idea have modified only SEP by artificial manipulation (e.g. photoperiod extensions), it is relevant to evaluate this hypothesis in populations segregating for this attribute in natural conditions. The aim of this work was to analyse the variability in SEP duration relative to AT in two F4 populations; in order to select contrasting phenotypes to evaluate the impact of this attribute on grain yield components and to analyse the selection response of this attribute. These segregating populations (Las Rosas INTA × Triguero 230 (A) and Klein Estrella × ProINTA B. Alazan (B)) were derived from parental lines with similar flowering time but different relative duration of their pre-anthesis phases. Two field experiments with previous vernalization treatment in cool chamber were carried out. In 2006, F4 populations were characterized and from one of them (population B, which presented higher variability) four groups were selected, which presented contrasting phenotypes in the attribute under study. Progenies of these groups (F5), together with remnant F4 full-sib of each one, were studied during 2007. Grain yield per plant was higher, due to GN increases, when duration of the SEP was lengthened. However, selection response to longer SEP with similar cycle to AT could not be found, possibly as the result of a high environmental influence on this attribute. The phenotypic variability evidenced in this attribute was not clearly associated with major adaptation genes evaluated (i.e. Ppd and/or Vrn), suggesting that other minor genes could be associated.

Nitrogen (N) fertilization plays a central role for improving yield in wheat and high N use efficiency (NUE) is desired to protect ground and surface waters. Several studies showed that sulfur (S) fertilization may increase NUE, but no attempts have been made to explain whether this increase is due to greater recovery efficiency (RE), an enhanced internal efficiency (IE) or by an improvement of both efficiencies. The aim of this study was to analyze the effects of different N and S fertilizer rates, and their interaction on N uptake, its partition at maturity, NUE and its main components. Field experiments were carried out during two consecutive growing seasons in the Argentinean Pampas using a single bread-wheat genotype grown under different combinations of N and S fertilizer rates. Additional experiments were performed in farmer fields using N and S fertilization evaluating different genotypes in order to analyze the components of NUE in other environmental conditions. Plant N uptake increased linearly in response to N addition until rates of ca. 80 kg N ha −1 . Sulfur addition showed no effect at the lowest N fertilizer rate, but N uptake was increased when S was applied at the highest N rate, revealing a synergism between both nutrients. At the lowest S rate RE was 42%, and increased to 70% when S fertilizer was added. No changes in IE in response to S fertilization were observed. These results were also observed in farmer field experiments, in genotypes that showed different IE. This study showed that S addition increased NUE mainly by increasing the N recovery from the soil. Thus, the concurrent management of N and S is important for reducing the potential pollution of residual soil nitrate by increasing N recovery from the soil while sustaining high nitrogen use efficiency.

The critical period for yield determination in barley ( Hordeum vulgare L.) is situated in the pre-heading phases. During the latest part of the critical period one of the most important yield components (i.e. the number of grains per spike) is set in two- and six-rowed barley. In wheat, much is known about the role of the spike in assimilate acquisition for the establishment of grains per spike, but not in barley. This paper evaluates how biomass partitioning between vegetative and reproductive organs impacts floret development and primordia survival in response to radiation during different periods in the crop cycle, in barley lines. Field experiments were carried out using two- and six-rowed near isogenic barley lines differing only in spike type. Shading treatments were applied at different periods during the crop cycle (from 60 to 15 days before and after heading) reducing the intercepted radiation (ca. 70%). Dynamics of floret primordia initiation and mortality and of floret development for different spikelet positions along the spike were measured, and biomass partitioning between vegetative and reproductive structures was calculated. Pre-heading shading reduced fertile florets per spike ( P The amount of assimilates partitioned to the spike at heading affected the number of fertile florets per spike in both barley types. However, when spike biomass at heading was corrected by nitrogen concentration, the fitness of the relationship did not improve in relation to the first one. In relative terms, radiation restrictions during the immediate pre-heading phase increased the amount of biomass partitioned to the growing spike.

In barley no studies have attempted to pinpoint the critical period for grain number determination, and it is frequently stated that the critical period is similar to that of wheat. However, there are important differences between the species and among barley genotypes (i.e. two- and six-rowed types) suggesting that this assumption requires testing. The objectives of this paper were (i) to determine the critical period for grain number determination in two- and six-rowed barleys, and (ii) to identify which yield components were more sensitive to changes in incident radiation during that period. Two field experiments were conducted using two pairs of near isogenic lines differing only in the spike type. Shading was imposed at different periods throughout the crop cycle (from 60 days before heading to 15 days after) to reduce incident solar radiation approximately 70%. The critical period for grain number determination tended to be slightly earlier in two- (ca. between 40 and 10 days before heading) than in six-rowed barleys (ca. between 30 days before heading until that stage). In terms of the external phenology, the beginning of the critical period for setting grains was 10 days after the beginning of stem elongation, and 10 days before flag leaf appearance in two- and six-rowed lines, respectively. Changes in the number of grains per unit area were correlated with crop growth rate during the critical period for yield determination.

Although floret abortion is related to a shortage in assimilates acquisition by the spikes, differences in floret abortion and grain setting has been reported between two- and six-rowed barley types. However, the origin of those differences has not been studied until now. This study was designed to explore the dynamics of floret development and to analyze the coordination between floret primordia development and growth of vegetative and reproductive organs in near isogenic barley lines differing only in the lateral spikelet fertility gene (Vrs1). Two field experiments using four near isogenic lines differing only in the spike type were carried out. Floret primordia development together with stem and spike growth were studied. In both barley types floret primordia mortality starts at the beginning of the spike active growth phase and continued when stem and spike growth were at their maximum rate. A reduced contribution of fertile florets in apical and basal spikelet positions explained the lower floret survival in six-rowed barleys. The chance to reach the fertile floret stage at heading was highly dependent on the floral development stage attained at the start of floret primordia mortality. Floret development stage at the beginning of active floret primordia mortality was higher for all spikelet positions in two- than six-rowed barleys. Grain setting was lower in six- than two-rowed genotypes associated to a lower grain setting in distal spikelet positions. Smaller carpels in six- than two-rowed genotypes in those positions could be associated with a reduce chance of those florets to set grains. The lower grain survival within the spike in six-rowed genotypes was caused by (i) a lower floret survival due to a lower degree of floret development in distal and lateral spikelet positions at the beginning of the floret primordia mortality stage and (ii) a reduced establishment of the fertile florets initially formed.

The manipulation of photoperiod sensitivity during stem elongation (terminal spikelet initiation—anthesis) has been suggested as a means to increase wheat yield potential. Nevertheless, photoperiod sensitivity and the interaction with vernalization during that phase have not been previously explored under field conditions. Thus, a field study was carried out (i) to assess whether duration of stem elongation is sensitive to photoperiod experienced only during that phase, and if so (ii) to evaluate whether vernalization interacts with this photoperiod response and (iii) to determine if altering duration of stem elongation may influence wheat yield and its components. The experiment was a factorial combination of two vernalization treatments (15 (V15) or 50 (V50) days at 4±1 °C and 8 h photoperiod), two photoperiod regimes during stem elongation (natural daylength in the field (NP+0) and one daylength extension of 6 h (NP+6)) and three cultivars (Buck Manantial (BM), Eureka Ferrocarril Sur (EFS), and ProINTA Puntal (PP)). The stem elongation phase responded to the actual photoperiod experienced during that phase in the three cultivars, increasing its duration with shorter photoperiod. In the vernalization-sensitive cultivars EFS and PP, plants vernalized 50 days showed greater response to photoperiod than those vernalized 15 days. In the most photoperiod-sensitive cultivar (BM), as duration of stem elongation lengthened by exposure to less inductive photoperiod, a higher number of fertile florets at anthesis was achieved leading to an increased grain number and thereby to a higher wheat yield. These results reinforce the idea that a longer duration of stem elongation may offer an alternative avenue to improve yield potential in wheat.

A longer duration of stem elongation (from terminal spikelet initiation to anthesis) would result in a higher wheat yield potential as a consequence of the increase in both spike dry weight and number of fertile florets at anthesis. It is not clearly understood, however, which processes are involved in the increased spike dry weight and whether this variable accounts for the higher number of fertile florets when duration of stem elongation is modified by photoperiod treatments. As vernalization interacts with photoperiod during the stem elongation phase, a better understanding of the role of vernalization is also required. A field experiment was carried out using two wheat cultivars (Buck Manantial (BM) and Eureka Ferrocarril Sur (EFS)) subjected to diverse periods of vernalization (V 15 and V 50 , plants vernalized for 15 and 50 days, respectively) and exposed to different photoperiods (NP+0 and NP+6, natural and 6 h extended photoperiod, respectively) only during the stem elongation phase. Floret development together with stem and spike growth during the stem elongation phase were studied. When the stem elongation phase was exposed to short photoperiod (i) duration of spike growth increased without any change in its growth rate, and (ii) most of the spike growth occurred during minimum stem growth rate as the onset of maximum stem growth rate was delayed. In EFS, the vernalization-sensitive cultivar, the former (i) and (ii) was true only when vernalization requirements were met. For both cultivars, the survival of the initiated floret primordia under short photoperiod increased, independently of vernalization. Both partitioning of assimilates to the spike and a direct photoperiod effect seemed to have been involved in the survival of floret primordia when the stem elongation phase was exposed to short photoperiod.

It has been established that photoperiod influences the rate of wheat development well beyond the end of the vegetative phase. Conversely, vernalization effects are still assumed to be mainly during the vegetative phase. The aim of the study was to assess the effects of different combinations of vernalization and photoperiods on the developmental characteristics of wheat pre-flowering reproductive phases under field conditions. For this purpose, three high yielding cultivars (ProINTA Puntal, ProINTA Super and Klein Pegaso) were subjected to two vernalization treatments (unvernalized (V0) and vernalized during 56 days in a cool chamber (V56)) and four photoperiod regimes. The photoperiod treatments consisted of the natural photoperiod of the season (NP+0) and daylength extensions over that of 2 (NP+2), 4 (NP+4), and 6 (NP+6) hours. Photoperiod influenced the duration of early (collar—first node detectable) and late (first node detectable—heading) pre-flowering reproductive phases, reducing their durations as photoperiod increased. When cultivars with strong vernalization response (ProINTA Puntal and ProINTA Super) were not vernalized, duration of the early and late pre-flowering reproductive phases increased and the rate of spikelet initiation decreased. Thus, when vernalization requirements were not satisfied, the spikelet initiation rate was even slower than the leaf initiation rate (both expressed in a thermal time base). Results showed significant interactions between photoperiod and vernalization (vegetative phase), or clear, though non-significant, trends to interactive effects (late pre-flowering reproductive phase). Thus, the length of vegetative and late pre-flowering reproductive phases changed in response to photoperiod depending on the level of satisfaction of the vernalization requirements. The relationship between the cumulative number of leaves on the main stem and thermal time fitted a bi-linear model whenever the final leaf number (LNf) was large, e.g. when photoperiod was not extended or vernalization requirements not satisfied. Thus, responses during the pre-flowering reproductive phases were accompanied by an increased phyllochron for leaves emerging after the first 7–8 leaves. This study demonstrated with field grown plants that the length of the late pre-flowering reproductive phase in wheat is sensitive to photoperiod and that the response to this environmental factor could be changed by the level of satisfaction of the vernalization requirements.

Two cultivars of each bread wheat ( Triticum aestivum L.), malting barley ( Hordeum vulgare L.) and spring rapeseed ( Brassica napus L.), were grown in a wide range of different sowing dates in Buenos Aires, Argentina. The aims of this study were (i) to analyse how the number of leaf primordia and the rate of leaf emergence interact to modify the duration of different pre-flowering phases and (ii) to establish how different yield components initiated during those phases are affected when the duration of these phases are modified by wide range of sowing dates. The results showed interactions between photoperiod and temperature for wheat and rapeseed producing different combination for the length of the pre-flowering phases. Barley reached floral initiation earlier than wheat and rapeseed and had a shorter ‘intrinsic earliness’. Plastochron and phyllochron varied among different sowing dates in the three species. In most cases the relationship between the cumulative number of leaves on the main shoot of wheat and barley and thermal time was describe by a bilinear model with the earlier leaves emerging faster than the later ones. However, the opposite pattern was observed for rapeseed which showed a slower rate of leaf emergence for the first than for the lasts leaves. The changes observed in the rate of leaf emergence throughout plant ontogeny, might delay or hasten the duration of the later reproductive phase during which the spikes grow and some of the initiated floret primordia becoming fertile. Extending the duration of the stem elongation phase in wheat and barley produced more fertile florets per spikelets.

The effects of Rht1 and Rht2 alleles on the dynamics of floret development in isogenic lines (dwarf, DD; semi-dwarf, SD and standard height, SH) of spring wheat were investigated. Studies were conducted on wheat grown in the field in each of 4 years and where water and nutrients were non-limiting. The number of grains per spike was significantly greater in the lines with Rht alleles than in the SH lines. Grain number for each line was such that DD>SD>SH. Grains per spike varied with the number of grains per spikelet rather than number of spikelets per spike. Grains per spikelet in turn varied with the number of fertile florets at anthesis. Florets were considered fertile when male and female reproductive organs had developed green anthers and bifidum stigma, respectively. The dwarfing genes had no effect on the percentage of fertile florets setting grain. Increased number of fertile florets per spikelet due to the presence of Rht1 and Rht2 alleles in the genome was a consequence of the higher number of relatively distal primordia, to progress to the stage of fertile floret at anthesis in the DD and SD than in the SH lines. This ability to allow that a greater proportion of distal florets maintain a normal rate of development was related to the fact that Rht alleles produced a more favourable assimilate partitioning to the spike during the pre-anthesis period associated with the reduction in stem growth imposed by Rht alleles. This allowed a higher proportion of the later-initiated floret primordia to produce fertile florets at anthesis.

This paper describes the effects of Rht alleles in isogenic lines of Maringa spring wheat (Triticum aestivum L.) on number of grains per spike, average grain weight and weight of individual grains from different positions within the spike. Plants were grown under five environmental conditions in the field (in the 1991, 1992 and 1993 growing seasons, with two sowing dates in the first two years), aiming to determine causes of lower average grain weight commonly observed in semi-dwarf (SD) compared with standard-height (SH) cultivars. The number of grains per spike was significantly greater in SD and dwarf (DD) lines than in the SH line due to differences among lines in number of grains per spikelet. Therefore, the relative contribution of proximal grains was affected negatively by Rht alleles while the contribution of distal grains was affected positively. Average grain weight was reduced with increases in the dose of Rht alleles, and this trait was negatively correlated to number of grains per spike. To understand the possible causes of this negative relationship, individual grains from specific positions within the spike were analysed. Basal grains in central spikelets were heavier than those in near apical and near basal spikelets. Within central spikelets, the ranking of individual grain weights were grains 2 > 1 > 3 > 4, numbered from the most proximal to the most distal positions. Proximal grains (1 and 2) were heaviest in SH, lightest in DD, and intermediate in the SD line. In general, the relative differences between the lines were smaller than that found for the average of all grains of the spike, in particular when grains of different positions within the central spikelets were considered. For all these grains, differences in weight due to Rht alleles were due to differences in rate of grain filling, with the effective duration of grain filling being almost unchanged. An increased source-sink relationship did not significantly modify the weight of any of the analysed grains in SD and SH lines (and just slightly increased rate of grain filling in the DD line). Therefore, the smaller grains in SD and DD lines could hardly have been due to an increased competition for assimilates. Alternatively, SD and DD lines had a greater proportion of grains from distal positions than the SH line, and these grains were always smaller than proximal grains. The reduction in average grain weight produced by Rht alleles therefore was due to a combination of effects on the potential size of each grain and on the contribution of grains from distal positions within the spike, with no effects attributed to an increased competition for assimilates.

Past breeding programs for increased wheat grain yield have increased the number of grains per m 2 , associated with a higher spike dry weight at anthesis. Other workers have shown that shading during the preanthesis period strongly reduced the number of grains per m 2 of the crop via reductions in the spike dry weight at anthesis. However, these effects have not been determined for cultivars of different potential number of grains per m 2 . A factorial field experiment was executed with three bread wheat cultivars released in 1920 (Klein Favorito), 1940 (Eureka FCS) and 1980 (Buck Pucara) in Argetina and two shading treatments (shading to 50% of the incoming solar radiation from the beginning of stem elongation to heading or no shading). Both cultivars and shading significantly affected the number of grains per m 2 , but their interaction was not significant. Cultivars differed only in number of grains per spikelet, while shading reduced both the number of spikes per m 2 and the number of grains per spike. The lack of a significant interaction indicates that the absolute reduction in grain number was similar for all cultivars, but the relative effect of shading was much greater for the old (low number of grains) cultivar than the modern one (62 and 42%, respectively). Preanthesis shading reduced number of grains per m 2 on main shoots less (45%) than on tillers (65%). Therefore, the relative contributions of these shoot categories to total number of grains per m 2 were strongly modified by shading. An evaluation of published data with conflicting findings on this shading effect suggests that the degree of modification observed has been related to the proportions of tiller and main shoot spikes.