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CD9 mRNA and protein expression levels in mouse slow frozen-rapid thawed oocytes were compared with those in fresh oocytes by using comparative quantitative real time reverse transcription-PCR and semiquantitative Western blot, respectively. The expression levels of both CD9 mRNA and protein in the frozen oocytes were significantly lower than those found in the fresh oocytes.

Objective: To incorporate conservative management of a heterotopic pregnancy using injection of KCl into the ectopic pregnancy (EP)., Design: A retrospective case report., Setting: A patient referred to an academic institution in the division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, incorporating the ultrasound (US) and operating room facilities., Intervention(s): Using US as a guide, KCl was injected into the chorionic cavity of an EP., Main Outcome Measure(s): Resolution of ectopic gestational tissue with resultant hematosalpinx requiring exploratory laparotomy. Uncomplicated prenatal course of intrauterine pregnancy., Result(s): Injection of KCl into the chorionic cavity of EP resulting in resolution of gestational tissue but complicated by hematosalpinx in the fallopian tube., Conclusion(s): Selective embryo reduction of a tubal heterotopic pregnancy remains a viable therapeutic option.