1. Serum insulin-like factor 3 is highly correlated with intratesticular testosterone in normal men with acute, experimental gonadotropin deficiency stimulated with low-dose human chorionic gonadotropin: a randomized, controlled trial.
- Author
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Roth MY, Lin K, Bay K, Amory JK, Anawalt BD, Matsumoto AM, Marck BT, Bremner WJ, and Page ST
- Subjects
- 17-alpha-Hydroxyprogesterone blood, Adolescent, Adult, Anti-Mullerian Hormone blood, Biomarkers blood, Dose-Response Relationship, Drug, Gonadotropin-Releasing Hormone antagonists & inhibitors, Humans, Inhibins blood, Male, Middle Aged, Oligopeptides pharmacology, Proteins, Young Adult, Chorionic Gonadotropin pharmacology, Gonadotropins deficiency, Insulin blood, Testis drug effects, Testis metabolism, Testosterone metabolism
- Abstract
Objective: To study the potential role for using serum biomarkers, including insulin-like factor 3 (INSL3), 17α-hydroxyprogesterone, antimüllerian hormone, and inhibin B, as correlates of intratesticular T (IT-T) concentrations in men., Design: Prospective, randomized, controlled trial., Setting: University-based medical center., Patient(s): Thirty-seven healthy men aged 18-50 years., Intervention(s): All men received the GnRH antagonist acyline, plus very low doses of hCG (0 IU, 15 IU, 60 IU, or 125 IU) SC every other day or 7.5 g T gel daily (75 mg delivered). The IT-T concentrations obtained by percutaneous testicular aspiration with simultaneous serum protein and steroid concentrations were measured at baseline and after 10 days of treatment., Main Outcome Measure(s): Intratesticular and serum hormone and gonadotropin concentrations., Result(s): After 10 days of gonadotropin suppression, serum INSL3 decreased by more than 90% and correlated highly with IT-T concentrations. In contrast, serum inhibin B, antimüllerian hormone, and 17α-hydroxyprogesterone did not correlate with IT-T. Serum INSL3 increased with the dose of hCG administered and returned to baseline after treatment., Conclusion(s): Serum INSL3 correlates highly with IT-T and serum T concentrations during acute gonadotropin suppression in men. Human chorionic gonadotropin stimulates dose-dependent increases in INSL3 and IT-T in healthy men and might be a useful biomarker of IT-T concentration in some clinical settings., Clinical Trial Registration Number: NCT# 00839319., (Copyright © 2013 American Society for Reproductive Medicine. All rights reserved.)
- Published
- 2013
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