6 results on '"Anaf V"'
Search Results
2. Estrogen and progesterone receptors in smooth muscle component of deep infiltrating endometriosis.
- Author
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Noël JC, Chapron C, Bucella D, Buxant F, Peny MO, Fayt I, Borghese B, and Anaf V
- Subjects
- Endometriosis pathology, Female, Follicular Phase metabolism, Humans, Intestinal Diseases pathology, Luteal Phase metabolism, Muscle, Smooth pathology, Stromal Cells metabolism, Stromal Cells pathology, Urinary Bladder Diseases pathology, Endometriosis metabolism, Intestinal Diseases metabolism, Muscle, Smooth metabolism, Receptors, Estrogen metabolism, Receptors, Progesterone metabolism, Urinary Bladder Diseases metabolism
- Abstract
Objective: To analyze the expression of estrogen (ER) and progesterone (PR) receptors in the smooth muscle component (SMC) of deep infiltrating endometriosis (DIE)., Design: A prospective clinical and pathologic study of 60 cases of DIE., Setting: University Hospital Department of Gynacology., Patient(s): Sixty patients with symptomatic DIE (uterosacral endometriosis n = 14; bladder endometriosis n = 10; colonic endometriosis n = 16; rectovaginal endometriosis n = 20)., Intervention(s): Laparoscopic surgery., Main Outcome Measure(s): The expression of ER and PR was studied by immunohistochemistry in the SMC directly around endometriotic foci and at distance (at least >1.5 cm) from them in correlation with proliferative and secretory phases of cycle., Results: The ER and PR were present in the SMC of DEI in each location excepting colonic endometriosis where ER were absent. Independently of cycle's phases the PR were more abundant than ER. With the exception of rectovaginal endometriosis, where the ER and PR were more abundant in the proliferative than in the secretory phase, in other locations the ER and PR did not differ significantly with cycle's phases. Last, if ER and PR were more abundant in SMC around endometriotic foci than at a distance from them. However, the difference was not significant., Conclusions: Our data substantially confirm for the first time that in various forms of DIE, ER and PR are present not only in glands and stroma but also in the smooth muscle major histologic component of this disease., (Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF
3. Lymph node involvement and lymphovascular invasion in deep infiltrating rectosigmoid endometriosis.
- Author
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Noël JC, Chapron C, Fayt I, and Anaf V
- Subjects
- Adult, Antibodies, Monoclonal immunology, Antibodies, Monoclonal metabolism, Antibodies, Monoclonal, Murine-Derived, Biomarkers metabolism, Endometriosis physiopathology, Endothelium, Lymphatic immunology, Endothelium, Lymphatic pathology, Female, Humans, Lymphatic Vessels immunology, Prospective Studies, Colon pathology, Endometriosis pathology, Lymph Nodes pathology, Lymphangiogenesis physiology, Lymphatic Vessels pathology, Rectum pathology
- Abstract
Objective: To analyze the lymph node involvement by endometriotic foci in rectosigmoid endometriosis and to correlate it with clinical and histological parameters including the presence of lymphovascular invasions, which could explain this lymph node involvement., Design: A prospective study of 26 consecutive cases of rectosigmoid endometriosis between January 2005 and January 2007., Setting: A multidisciplinary study including clinical and pathological data., Patient(s): Twenty-six patients with symptomatic rectosigmoid endometriosis., Intervention(s): Laparoscopic surgery with pathological analysis of each specimen., Main Outcome Measure(s): Involvement of lymph nodes by endometriotic foci was correlated with the size and the wall layers affected by endometriotic lesions, the number of lymph nodes retrieved, and the presence of lymphovascular invasions demonstrated by D2-40, a specific antibody to lymphatic vessels., Result(s): Lymph node involvement by endometriosis was observed in 11 of the 26 patients (42.3%) and correlated with the size of the lesions, the number of lymph nodes retrieved, and the presence of lymphovascular invasions, which were observed in 36.3% of cases., Conclusion(s): Our data confirm that lymph node involvement by endometriotic foci is a frequent event in rectosigmoid endometriosis and may result at least partially from a lymphatic spread of the disease.
- Published
- 2008
- Full Text
- View/download PDF
4. Pain, mast cells, and nerves in peritoneal, ovarian, and deep infiltrating endometriosis.
- Author
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Anaf V, Chapron C, El Nakadi I, De Moor V, Simonart T, and Noël JC
- Subjects
- Adult, Endometriosis complications, Female, Humans, Hyperalgesia etiology, Ovarian Diseases complications, Ovary innervation, Ovary pathology, Peritoneal Diseases complications, Peritoneum innervation, Peritoneum pathology, Endometriosis pathology, Hyperalgesia pathology, Mast Cells pathology, Ovarian Diseases etiology, Peritoneal Diseases pathology
- Abstract
Objective: To detect and quantify mast cells in peritoneal, ovarian, and deep infiltrating endometriosis and to study the relationship between mast cells and nerves in endometriosis., Design: Prospective histological and immunohistochemical study., Setting: University of Brussels, Belgium., Patient(s): Sixty-nine women undergoing laparoscopic excision of endometriosis for pain. Thirty-seven biopsies of normal tissue were obtained from women without endometriosis., Intervention(s): Excision of endometriosis from different anatomical locations., Main Outcome Measure(s): Immunohistochemistry with chymase and tryptase to confirm the presence of mast cells and activated mast cells, respectively, in endometriotic lesions. Quantification of mast cells, activated mast cells, and degranulating mast cells in the different locations of endometriosis. Study of the relationship between mast cells and nerves by quantifying mast cells located less than 25 mum from nerves immunohistochemically stained with S-100 protein. Preoperative pain score evaluation by visual analogue scales., Result(s): Patients with deeply infiltrating lesions had significantly higher preoperative pain scores than patients with peritoneal or ovarian endometriosis. Mast cells and degranulating mast cells are significantly more abundant in endometriotic lesions than in nonaffected tissues. Deep infiltrating lesions show a significantly higher number of mast cells, activated mast cells, and mast cells located <25 microm from nerves than peritoneal and ovarian lesions. We found significantly more degranulating mast cells in deep infiltrating lesions than in peritoneal lesions., Conclusion(s): The presence of increased activated and degranulating mast cells in deeply infiltrating endometriosis, which are the most painful lesions, and the close histological relationship between mast cells and nerves strongly suggest that mast cells could contribute to the development of pain and hyperalgesia in endometriosis, possibly by a direct effect on nerve structures.
- Published
- 2006
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5. Ovarian endometrial cysts: the role of gonadotropin-releasing hormone agonist and/or drainage.
- Author
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Donnez J, Nisolle M, Gillerot S, Anaf V, Clerckx-Braun F, and Casanas-Roux F
- Subjects
- Adult, Biopsy, Endometriosis pathology, Female, Humans, Laparoscopy, Ovarian Cysts pathology, Ovarian Diseases pathology, Ovary pathology, Prospective Studies, Drainage, Endometriosis therapy, Goserelin therapeutic use, Ovarian Cysts therapy, Ovarian Diseases therapy
- Abstract
Objective: To evaluate the role of GnRH agonist (GnRH-a) and/or drainage in the management of large endometriomas., Design: This prospective clinical study was conducted in a parallel and randomized design., Setting: Department of Gynecology, Cliniques Universitaires St. Luc, Brussels, Belgium., Patients: Eight infertile women with laparoscopically confirmed ovarian endometriotic cysts. After laparoscopic drainage of the ovarian cyst, patients were randomized. Patients in group I (n = 40) received no therapy. Patients in group II (n = 40) received GnRH-a therapy for 12 weeks. A second-look laparoscopy was performed after 12 weeks in each woman., Results: After drainage, a quick recurrence of the endometrial cyst was observed in only group I. Indeed, the score and the cyst size were similar to the values observed before the first laparoscopy. In group II, a significant decrease in score and cyst diameter was observed. Ovarian biopsies revealed significant reduction in the stromal vascularization and a significant reduction in the mitotic activity in the group of women treated with GnRH-a., Conclusion: The quick recurrence of the ovarian cyst after drainage proved that drainage alone is ineffective. Drainage followed by GnRH-a was effective in the reduction of cyst size and the glandular mitotic activity.
- Published
- 1994
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- View/download PDF
6. Morphometric study of the stromal vascularization in peritoneal endometriosis.
- Author
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Nisolle M, Casanas-Roux F, Anaf V, Mine JM, and Donnez J
- Subjects
- Epithelium blood supply, Female, Humans, Mitotic Index, Peritoneum blood supply, Stromal Cells pathology, Endometriosis pathology, Peritoneal Neoplasms blood supply, Peritoneal Neoplasms pathology
- Abstract
To evaluate the stromal vascularization of different appearances of peritoneal endometriosis, biopsies were taken from peritoneal areas with endometriosis in a series of 135 infertile women and classified as typical (black), red, or white lesions. The number of capillaries per mm2 of stroma, their mean surface area, and the ratio of capillaries/stroma surface area, and the mitotic activity were analyzed in typical, red, and white lesions. Significant differences were found between the different subgroups. The higher vascularization and mitotic activity observed in red lesions suggested the hypothesis that such lesions are very active and probably the first stage of early implantation of endometrial glands and stroma. The poor vascularization and the absence of mitosis observed in white lesions suggested that these lesions are much less active than red lesions and are a quiescent stage of the disease. Our study proves that the "activity" of peritoneal endometriosis is related to the vascularity. This concept must be considered in the further discussion of American Fertility Society Endometriosis Classification. Typical, red and white lesions are three different stages of the peritoneal disease and their relative relation to infertility is also probably different.
- Published
- 1993
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