1. In vitro effects of phthalate esters in human myometrial and leiomyoma cells and increased urinary level of phthalate metabolite in women with uterine leiomyoma.
- Author
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Jin Hee Kim, Sung Hoon Kim, Young Sang Oh, Hyo Jin Ihm, Hee Dong Chae, Chung-Hoon Kim, Byung Moon Kang, Kim, Jin Hee, Kim, Sung Hoon, Oh, Young Sang, Ihm, Hyo Jin, Chae, Hee Dong, Kim, Chung-Hoon, and Kang, Byung Moon
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PHTHALATE esters , *UTERINE contraction , *SMOOTH muscle tumors , *URINARY organ diseases , *BCL-2 proteins , *CELL metabolism , *PROTEIN metabolism , *ACADEMIC medical centers , *APOPTOSIS , *BIOCHEMISTRY , *CELL lines , *CELL physiology , *CELLS , *COLLAGEN , *LONGITUDINAL method , *PHENOMENOLOGY , *MYOMETRIUM , *RISK assessment , *TIME , *UTERINE fibroids , *UTERINE tumors , *CASE-control method , *CARBOCYCLIC acids , *CANCER cell culture , *METABOLISM - Abstract
Objective: To investigate the possible role of phthalate, a ubiquitous chemical used in consumer products, in the pathogenesis of uterine leiomyoma.Design: Experimental and prospective case-control study using human samples.Setting: University hospital.Patient(s): Fifty-three women with histologic evidence of uterine leiomyoma and 33 surgical controls without leiomyoma.Intervention(s): Human myometrial and leiomyoma cells were treated with di-(2-thylhexyl)-phthalate (DEHP).Main Outcome Measure(s): Cell viability assay and Western blot analyses after in vitro DEHP treatment; high-performance liquid chromatography electrospray ionization tandem mass spectrometry in cases and controls.Result(s): In vitro treatment with DEHP led to an increased viability and increased expressions of proliferating cell nuclear antigen, B-cell lymphoma 2 protein, and type I collagen in myometrial and leiomyoma cells. The urinary concentration of mono-(2-ethyl-5-carboxypentyl) phthalate was higher in women with leiomyoma compared with controls.Conclusion(s): These findings suggest that exposure to phthalate may play a role in the pathogenesis of uterine leiomyoma by enhancing proliferative activity, exerting an antiapoptotic effect, and increasing collagen contents in myometrial and leiomyoma cells. [ABSTRACT FROM AUTHOR]- Published
- 2017
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