1. Effect of chilling on the organization of tubulin and chromosomes in rhesus monkey oocytes.
- Author
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Songsasen, Nucharin, Yu, Il Jeoung, Ratterree, Marion S, VandeVoort, Catherine A, and Leibo, Stanley P
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ANIMAL experimentation , *CELL physiology , *CHROMOSOMES , *COLD (Temperature) , *COMPARATIVE studies , *RESEARCH methodology , *MEDICAL cooperation , *NERVE tissue proteins , *OVUM , *POLYMERS , *PRIMATES , *RESEARCH , *TIME , *EVALUATION research - Abstract
Objective: To determine the effects of chilling on the organization and distribution of tubulin and chromosomes in rhesus monkey oocytes.Design: Comparative laboratory study.Setting: Academic research laboratory.Animal(s): Eight adult female rhesus monkeys (Macaca mulatta) aged 6-16 years.Intervention(s): A total of 171 oocytes retrieved from eight rhesus monkeys were separated into nine groups. One group of control oocytes was held at 37 degrees C during the experiment. Four groups of oocytes were rapidly cooled to 0 degrees C and held for 1, 5, 10, or 30 minutes and then fixed and stained. Four other groups of oocytes were cooled to 0 degrees C, held for 1, 5, 10, or 30 minutes, warmed and incubated at 37 degrees C for 60 minutes, and then fixed and stained.Main Outcome Measure(s): Organization of cytoskeleton and chromosomes.Result(s): Exposure of rhesus oocytes to 0 degrees C for only 1 minute resulted in complete depolymerization of tubulin. Incubation of chilled oocytes at 37 degrees C for 60 minutes caused partial restoration of tubulin, although most oocytes exhibited abnormal alignment of chromosomes and disorganized meiotic spindles.Conclusion(s): We conclude that rhesus monkey oocytes are extremely sensitive to chilling injury. Their successful cryopreservation may require rapid cooling to outpace this injury. [ABSTRACT FROM AUTHOR]- Published
- 2002
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