1. Microsatellites in the mitochondrial genome ofPhytophthora cinnamomifailed to provide highly polymorphic markers for population genetics
- Author
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Philip A. O’Brien, I.C. Tommerup, and Mark P. Dobrowolski
- Subjects
Genetic Markers ,Phytophthora ,Mitochondrial DNA ,Molecular Sequence Data ,Population ,Phytophthora cinnamomi ,DNA, Mitochondrial ,Polymerase Chain Reaction ,Microbiology ,Genome ,Genetics ,Cloning, Molecular ,DNA, Fungal ,education ,Molecular Biology ,Electrophoresis, Agar Gel ,mtDNA control region ,education.field_of_study ,Base Sequence ,biology ,fungi ,food and beverages ,biology.organism_classification ,Genetic marker ,Microsatellite ,Electrophoresis, Polyacrylamide Gel ,Microsatellite Repeats - Abstract
Microsatellites were evaluated as genetic markers for the mitochondrial genome (mtDNA) of Phytophthora cinnamomi for population studies. Two (A)n microsatellite loci were cloned from the mtDNA of P. cinnamomi. Amplification products from these loci showed little polymorphism among Phytophthora isolates due to their location in coding regions of mtDNA. A further three (A)n microsatellite loci obtained from the complete mtDNA sequence of P. infestans were also not highly polymorphic, although located in non-coding mtDNA. The presence of the (A)n microsatellites was not conserved in the genus Phytophthora. Unlike those of the chloroplast genome of plants, (A)n microsatellite loci of mtDNA do not have potential as highly polymorphic markers in Phytophthora.
- Published
- 1998
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