1. Photosensitizing activity of water- and lipid-soluble phthalocyanines on Escherichia coli
- Author
-
Francesca Rossi, Giulio Jori, Johan E. van Lier, Giuliana Valduga, and Giulio Bertolini
- Subjects
DNA, Bacterial ,Gram-negative bacteria ,Indoles ,Light ,Isoindoles ,medicine.disease_cause ,Microbiology ,Cell membrane ,chemistry.chemical_compound ,Photosensitivity ,Bacterial Proteins ,Genetics ,medicine ,Escherichia coli ,Organometallic Compounds ,Molecular Biology ,Edetic Acid ,biology ,Chemistry ,Membrane Proteins ,biology.organism_classification ,Zinc ,Membrane ,medicine.anatomical_structure ,Biochemistry ,Solubility ,Zinc Compounds ,Phthalocyanine ,Electrophoresis, Polyacrylamide Gel ,Transformation, Bacterial ,Bacterial outer membrane ,Bacteria ,Bacterial Outer Membrane Proteins - Abstract
Escherichia coli, as most Gram-negative bacteria, is insensitive to the photosensitizing action of both lipid-soluble Zinc-phthalocyanine (Zn-Pc) and water-soluble Zinc-mono/disulfonated phthalocyanine (Zn-PcS). Photosensitivity can be induced by alteration of the outer membrane, as obtained by either induction of competence or treatment with Tris-EDTA. Both phthalocyanines largely bind at the level of the cytoplasmic membrane; however, Zn-PcS shows a superior photosensitizing activity as compared with Zn-Pc. Biochemical analyses performed on irradiated cells suggest that the cytoplasmic membrane is an important target of the photoprocess, while DNA is not involved.
- Published
- 1990