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Start Over Author "Fournier, Jean-Michel" Journal fems microbiology letters
5 results on '"Fournier, Jean-Michel"'

3. Usefulness of R72H PCR assay for differentiation between Vibrio parahaemolyticus and Vibrio alginolyticus species: validation by DNA–DNA hybridization

Author

Robert-Pillot, Annick, Guenole, Alain, and Fournier, Jean-Michel

Subjects
POLYMERASE chain reaction, DNA
Abstract

We compared the efficiencies of biochemical methods and polymerase chain reaction (PCR) for the identification of Vibrio parahaemolyticus strains. The 122 isolates studied, identified by biochemical tests as V. parahaemolyticus or Vibrio alginolyticus, were tested by R72H PCR assay. The results obtained with the two methods were consistent for 90% of the strains studied. PCR amplification of the R72H fragment generated two unique amplicons, 387 bp and 320 bp in length. For 11% of the strains from seawater, the results of biochemical identification did not correlate with PCR results. DNA–DNA hybridization experiments provided evidence that some strains identified as V. alginolyticus in biochemical tests should be considered members of the V. parahaemolyticus species. We therefore suggest that biochemical tests are not accurate enough for the identification of V. parahaemolyticus isolates and we demonstrate that amplification of the R72H fragment, whether the amplicon is 320 bp or 387 bp long, is a powerful tool for the reliable identification of V. parahaemolyticus. [Copyright &y& Elsevier]

Published
2002
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4. Genetic analysis of the cap5locus of Staphylococcus aureus

Author

Wann, Elisabeth R, Dassy, Bruno, Fournier, Jean‐Michel, and Foster, Timothy J

Abstract

Staphylococcus aureusexpresses at least eight distinct serotypes of capsular polysaccharide (CP). Gene clusters involved in the expression of serotypes 1, 5 and 8 have been cloned and sequenced. In this report we describe the isolation and analysis of serotype 5 capsular polysaccharide‐defective mutants. A naturally occurring capmutation in the laboratory strains 8325‐4 and RN4220 was mapped to the cap5Egene by genetic complementation. The cap5H–Kgenes were shown to be responsible for CP5 serotype specificity by transduction and complementation.

Published
1999
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5. Genetic analysis of the cap5 locus of Staphylococcus aureus

Author

Wann, Elisabeth R., Dassy, Bruno, Fournier, Jean-Michel, and Foster, Timothy J.

Abstract

Staphylococcus aureus expresses at least eight distinct serotypes of capsular polysaccharide (CP). Gene clusters involved in the expression of serotypes 1, 5 and 8 have been cloned and sequenced. In this report we describe the isolation and analysis of serotype 5 capsular polysaccharide-defective mutants. A naturally occurring cap mutation in the laboratory strains 8325-4 and RN4220 was mapped to the cap5E gene by genetic complementation. The cap5H–K genes were shown to be responsible for CP5 serotype specificity by transduction and complementation.

Published
1999
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