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Start Over Author "Fengfeng Xu" Journal fems microbiology letters
3 results on '"Fengfeng Xu"'

1. Construction of a family of lactococcal vectors for gene cloning and translational fusion

Author

Lindsay E. Pearce, Pak-Lam Yu, and Fengfeng Xu

Subjects
Cloning, Genetics, Base Sequence, Genetic Vectors, Molecular Sequence Data, Restriction Mapping, Molecular cloning, Biology, Origin of replication, Microbiology, Lactococcus lactis, Plasmid, Lac Operon, Genes, Bacterial, Protein Biosynthesis, Escherichia coli, Multiple cloning site, Replicon, Vector (molecular biology), Cloning, Molecular, Molecular Biology, Gene, Plasmids
Abstract

A family of stable lactococcal vectors have been constructed based on the pFX1 replicon using either the alpha fragment or the complete Escherichia coli lacZ gene as a selective marker. These vectors also incorporate multiple cloning sites and examples are given of their use for gene cloning and translational fusion studies in lactococci.

Published
1991
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