1. Isolation of genes expressed during the developmental stages of the oyster mushroom,Pleurotus ostreatus, using expressed sequence tags
- Author
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Seung Ho Lee, Yong-Sup Cho, Eun-Suk Son, Chang-Soo Lee, Nam-Kuk Kim, Jin-sung Lee, Ki-Hwan Kim, Woong-Han Youn, Beom-Gi Kim, Joongho Joh, Young-Bok Yoo, and Sung-Ja Jeong
- Subjects
Basidiospore ,Genes, Fungal ,Pleurotus ,Microbiology ,Genome ,Gene Expression Regulation, Fungal ,Botany ,Genetics ,Fruiting Bodies, Fungal ,RNA, Messenger ,DNA, Fungal ,Molecular Biology ,Gene ,Gene Library ,Expressed Sequence Tags ,Expressed sequence tag ,Mushroom ,Mycelium ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,cDNA library ,Gene Expression Profiling ,food and beverages ,RNA, Fungal ,Spores, Fungal ,biology.organism_classification ,Pleurotus ostreatus - Abstract
In order to isolate and identify the developmentally regulated genes during fruiting body development, cDNA libraries were constructed from eight developmental stages of the Oyster mushroom, Pleurotus ostreatus. From these libraries, 11 761 expressed sequence tags (PoESTs) were generated. Of these, 4060 different genes (PoUnigenes) were identified, representing 34.5% of the entire genome. Redundancy analysis of ESTs during the developmental stages identified eight, 13 and two genes that were specifically expressed in mycelia, fruiting body and basidiospore, respectively. RT-PCR was used to confirm the specific expression of nine genes which showed specific redundancy in fruiting body stages, four genes of which were expressed specifically in fruiting body stages as expected in redundancy analysis, and other genes were expressed abundantly in fruiting body stages. The EST database of P. ostreatus generated during this study provides a genetic and biochemical basis for future studies of the developmental stages of basidiomycetes.
- Published
- 2007