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Start Over Descriptor "Continuous Cultures" Journal fems microbiology ecology
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1. Major changes in the composition of a Southern Ocean bacterial community in response to diatom-derived dissolved organic matter.

Author

Landa, Marine, Blain, Stéphane, Harmand, Jércome, Monchy, Sébastien, Rapaport, Alain, and Obernosterer, Ingrid

Subjects
*DISSOLVED organic matter, *BACTERIAL communities, *DIATOMS, *HETEROTROPHIC bacteria, *PHYTOPLANKTON
Abstract

In the Southern Ocean, natural iron fertilization in the wake of islands leads to annually occurring spring phytoplankton blooms associated with enhanced heterotrophic activity through the release of labile dissolved organic matter (DOM). The aim of this study was to investigate experimentally how diatom-derived DOM affects the composition of Southern Ocean winter water bacterial communities and to identify the most responsive taxa. A bacterial community collected in the naturally iron-fertilized region off Kerguelen Island (KEOPS2 October-November 2011) was grown onboard in continuous cultures, on winter water alone or amended with diatom-derived DOM supplied at identical DOC concentrations. 454 sequencing of 16S amplicons revealed that the two DOM sources sustained strikingly different bacterial communities, with higher relative abundances of Sulfitobacter, Colwellia and Methylophaga operational taxonomic units (OTUs) and lower relative abundances of Polaribacter, Marinobacter, NAC11-7 and SAR11 OTUs in diatom-DOM compared to winter water conditions. Using a modeling approach, we obtained growth rates for phylogenetically diverse taxa varying between 0.12 and 0.49 d-1 under carbon-limited conditions. Our results identify diatom DOM as a key factor shaping Southern Ocean winter water bacterial communities and suggest a role for niche partitioning and microbial interactions in organic matter utilization. [ABSTRACT FROM AUTHOR]

Published
2018
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2. Major changes in the composition of a Southern Ocean bacterial community in response to diatom-derived dissolved organic matter

Author

Alain Rapaport, Sébastien Monchy, Marine Landa, Jérôme Harmand, Ingrid Obernosterer, Stéphane Blain, Laboratoire d'Océanographie Microbienne (LOMIC), Observatoire océanologique de Banyuls (OOB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), University of California [Santa Cruz] (UCSC), University of California, Laboratoire de Biotechnologie de l'Environnement [Narbonne] (LBE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA), Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 (LOG), Institut national des sciences de l'Univers (INSU - CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Mathématiques, Informatique et STatistique pour l'Environnement et l'Agronomie (MISTEA), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences de l'Univers (INSU)-Les Enveloppes Fluides et l'Environnement (LEFE)-Cycles Biogeochimiques, Environnement et Ressources (CYBER), French Ministry of Science, ANR-10-BLAN-0614,KEOPS 2,Kerguelen : Comparaison plateau Ocean2(2010), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA), Université du Littoral Côte d'Opale-Université de Lille-Centre National de la Recherche Scientifique (CNRS), University of California [Santa Cruz] (UC Santa Cruz), University of California (UC), Institut national des sciences de l'Univers (INSU - CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Nord]), Université de Lille-Université du Littoral Côte d'Opale-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Observatoire océanologique de Banyuls (OOB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut national des sciences de l'Univers (INSU - CNRS)

Subjects
0301 basic medicine, 020209 energy, [MATH.MATH-DS]Mathematics [math]/Dynamical Systems [math.DS], 030106 microbiology, Iron fertilization, Heterotroph, 02 engineering and technology, [SDV.BID]Life Sciences [q-bio]/Biodiversity, Biology, Applied Microbiology and Biotechnology, Microbiology, 03 medical and health sciences, Bacterial community composition, Phytoplankton, Dissolved organic carbon, 0202 electrical engineering, electronic engineering, information engineering, Dissolved organic matter, Organic matter, 14. Life underwater, Organic Chemicals, Southern Ocean, Indian Ocean, 16S rRNA gene sequencing, 030304 developmental biology, Alphaproteobacteria, [SDU.OCEAN]Sciences of the Universe [physics]/Ocean, Atmosphere, Diatoms, chemistry.chemical_classification, 0303 health sciences, Ecology, fungi, Niche differentiation, Continuous cultures, Marinobacter, biology.organism_classification, [INFO.INFO-MO]Computer Science [cs]/Modeling and Simulation, Carbon, 030104 developmental biology, Diatom, chemistry, Seasons, Flavobacteriaceae, Gammaproteobacteria
Abstract

In the Southern Ocean, natural iron fertilization in the wake of islands leads to annually occurring spring phytoplankton blooms associated with enhanced heterotrophic activity through the release of labile dissolved organic matter (DOM). The aim of this study was to investigate experimentally how diatom-derived DOM affects the composition of Southern Ocean winter water bacterial communities and to identify the most responsive taxa. A bacterial community collected in the naturally iron-fertilized region off Kerguelen Island (KEOPS2 October-November 2011) was grown onboard in continuous cultures, on winter water alone or amended with diatom-derived DOM supplied at identical DOC concentrations. 454 sequencing of 16S amplicons revealed that the two DOM sources sustained strikingly different bacterial communities, with higher relative abundances of Sulfitobacter, Colwellia and Methylophaga operational taxonomic units (OTUs) and lower relative abundances of Polaribacter, Marinobacter, NAC11-7 and SAR11 OTUs in diatom-DOM compared to winter water conditions. Using a modeling approach, we obtained growth rates for phylogenetically diverse taxa varying between 0.12 and 0.49 d-1 under carbon-limited conditions. Our results identify diatom DOM as a key factor shaping Southern Ocean winter water bacterial communities and suggest a role for niche partitioning and microbial interactions in organic matter utilization.

Published
2018

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