Search

Your search keyword '"Cheng-Wen, Lin"' showing total 5 results
Start Over Author "Cheng-Wen, Lin" Journal fems immunology & medical microbiology
5 results on '"Cheng-Wen, Lin"'

1. Severe acute respiratory syndrome coronavirus 3C-like protease-induced apoptosis

Author

Shi-Yi Shiu, Kuan-Hsun Lin, Cheng Wen Lin, Tsung-Han Hsieh, and Jeng-Yi Li

Subjects
Microbiology (medical), Programmed cell death, viruses, Immunology, Caspase 3, Biology, Severe Acute Respiratory Syndrome, Transfection, medicine.disease_cause, Microbiology, Cell Line, Promonocyte, Pathogenesis, Viral Proteins, medicine, Humans, Immunology and Allergy, Annexin A5, skin and connective tissue diseases, Research Articles, Coronavirus, reactive oxygen species, chemistry.chemical_classification, Reactive oxygen species, nuclear factor-kappa B signalling, Rhodamines, Activator (genetics), apoptosis, 3C Viral Proteases, NF-kappa B, 3CLpro, General Medicine, Molecular biology, Caspase 9, Transcription Factor AP-1, Cysteine Endopeptidases, Infectious Diseases, SARS-coronavirus, Microscopy, Fluorescence, Severe acute respiratory syndrome-related coronavirus, chemistry, Apoptosis, Caspases, Signal Transduction
Abstract

The pathogenesis of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is an important issue for the treatment and prevention of severe acute respiratory syndrome. Recently, SARS-CoV has been demonstrated to induce cell apoptosis in Vero-E6 cells. The possible role of SARS-CoV 3C-like protease (3CLpro) in virus-induced apoptosis is characterized in this study. Growth arrest and apoptosis via caspase-3 and caspase-9 activities were demonstrated in SARS-CoV 3CLpro-expressing human promonocyte cells. The fluorescence intensity of dihydrorhodamine 123 staining indicated that cellular reactive oxygen species were markedly increased in SARS-CoV 3CLpro-expressing cells. Moreover, in vivo signalling pathway assay indicated that 3CLpro increased the activation of the nuclear factor-kappa B-dependent reporter, but inhibited activator protein-1-dependent transcription. This finding is likely to be responsible for virus-induced apoptotic signalling.

Published
2006
Full Text
View/download PDF

2. Prevalence of human herpesvirus 8 DNA in peripheral blood mononuclear cells of acute and chronic leukemia patients in Taiwan: Table 1

Author

Cheng Wen Lin, Fang-Yan Wu, Chao-Hsien Chen, Ching-Tien Peng, Chun-Pin Chang, and Chao-Li Liu

Subjects
Microbiology (medical), Acute leukemia, biology, business.industry, viruses, Immunology, virus diseases, Cancer, General Medicine, medicine.disease, medicine.disease_cause, biology.organism_classification, Microbiology, Peripheral blood mononuclear cell, Virology, Herpesviridae, Virus, Leukemia, Infectious Diseases, Chronic leukemia, medicine, Immunology and Allergy, Gammaherpesvirinae, business
Abstract

Human herpesvirus 8 (HHV-8) is associated with the development of Kaposi's sarcoma and several other human malignancies. The prevalence of HHV-8 DNA in peripheral blood mononuclear cells (PBMCs) in Taiwanese leukemia populations has not been investigated. In this study, HHV-8 DNA was extracted from PBMCs, and detected in 10.29% of the leukemia cases and 8.94% of the relatives' cases. In addition, the prevalence of HHV-8 DNA in PBMCs was nonsignificantly associated with gender, age and leukemia subtypes. The study examines the prevalence of HHV-8 DNA in PBMCs in Taiwanese leukemia and can be applied in further epidemiological studies.

Published
2011
Full Text
View/download PDF

3. Comparative prevalence of plasma human herpesvirus 8 DNA in sexual contact and intravenous injection routes of HIV transmission

Author

Chao-Li Liu, Fang-Yang Wu, Chun-Pin Chang, and Cheng Wen Lin

Subjects
Microbiology (medical), Simplexvirus, food.ingredient, viruses, Immunology, HIV Infections, medicine.disease_cause, Microbiology, Virus, Herpesviridae, Plasma, food, Prevalence, medicine, Humans, Immunology and Allergy, Gammaherpesvirinae, Substance Abuse, Intravenous, Sarcoma, Kaposi, AIDS-Related Opportunistic Infections, biology, virus diseases, Sexually Transmitted Diseases, Viral, General Medicine, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Virology, Infectious Diseases, Lytic cycle, DNA, Viral, Herpesvirus 8, Human, Injections, Intravenous, Lentivirus, HIV-1, biology.protein, RNA, Viral, Antibody, Viral load
Abstract

Detection of plasma human herpesvirus (HHV)-8 DNA correlates with antibodies to lytic HHV-8 antigens, being predictive of Kaposi's sarcoma in HIV-infected patients. We show that the prevalence of plasma HHV-8 DNA was 10.6% for HIV infection through sexual contact and 7.1% for HIV infection through intravenous injection. In addition, the prevalence of plasma HHV-8 DNA was significantly associated with male gender (9.4%) and HIV viral load below 1000 copies mL(-1) (12.1%), but not age or CD4 cell count in HIV-infected patients. The study suggested that detection of plasma HHV-8 DNA could be important for monitoring replicating HHV-8 in HIV-infected patients, and may have use as a marker for the diagnosis of HHV-8 infection in blood-borne transmission.

Published
2008
Full Text
View/download PDF

4. Severe acute respiratory syndrome coronavirus 3C-like protease-induced apoptosis.

Author

Cheng-Wen Lin, Kuan-Hsun Lin, Tsung-Han Hsieh, Shi-Yi Shiu, and Jeng-Yi Li

Subjects
*APOPTOSIS, *CELL death, *CORONAVIRUSES, *CORONAVIRUS diseases, *IMMUNOLOGY, *MEDICAL microbiology
Abstract

The pathogenesis of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is an important issue for the treatment and prevention of severe acute respiratory syndrome. Recently, SARS-CoV has been demonstrated to induce cell apoptosis in Vero-E6 cells. The possible role of SARS-CoV 3C-like protease (3CLpro) in virus-induced apoptosis is characterized in this study. Growth arrest and apoptosis via caspase-3 and caspase-9 activities were demonstrated in SARS-CoV 3CLpro-expressing human promonocyte cells. The fluorescence intensity of dihydrorhodamine 123 staining indicated that cellular reactive oxygen species were markedly increased in SARS-CoV 3CLpro-expressing cells. Moreover, in vivo signalling pathway assay indicated that 3CLpro increased the activation of the nuclear factor-kappa B-dependent reporter, but inhibited activator protein-1-dependent transcription. This finding is likely to be responsible for virus-induced apoptotic signalling. [ABSTRACT FROM AUTHOR]

Published
2006
Full Text
View/download PDF

5. Comparative prevalence of plasma human herpesvirus 8 DNA in sexual contact and intravenous injection routes of HIV transmission.

Author

Cheng-Wen Lin, Chun-Pin Chang, Fang-Yang Wu, and Chao-Li Liu

Subjects
*HERPESVIRUS diseases, *KAPOSI'S sarcoma, *SARCOMA, *HIV infections, *HIV-positive persons, *DNA, *BLOODBORNE infections, *PLASMA cells, *INFECTIOUS disease transmission
Abstract

Detection of plasma human herpesvirus (HHV)-8 DNA correlates with antibodies to lytic HHV-8 antigens, being predictive of Kaposi's sarcoma in HIV-infected patients. We show that the prevalence of plasma HHV-8 DNA was 10.6% for HIV infection through sexual contact and 7.1% for HIV infection through intravenous injection. In addition, the prevalence of plasma HHV-8 DNA was significantly associated with male gender (9.4%) and HIV viral load below 1000 copies mL−1 (12.1%), but not age or CD4 cell count in HIV-infected patients. The study suggested that detection of plasma HHV-8 DNA could be important for monitoring replicating HHV-8 in HIV-infected patients, and may have use as a marker for the diagnosis of HHV-8 infection in blood-borne transmission. [ABSTRACT FROM AUTHOR]

Published
2008
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results