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Using molecular technique to explore the mechanism of hedgehog hide and other tissues applied as traditional Chinese medicine, and promote molecular evolution of Erinaceus amurensis in the Far East, taking the complete mitochondrial genome sequence of Erinaceus amurensis as the material, 12 non- repeating coding sequences (CDS) with a length greater than 300 bp were selected as the research objects in this study, and their codon preference was analyzed by using CodonW1.4.2, SPSS 25.0, Excel 2007 and other software. The average GC content of the third codon was 24.30%; the number of effective codons (ENC) ranged from 31.83 to 50.67, with an average value of 43.37; and the relative synonymous codon usage (RSCU) value of 32 codons was greater than 1.00, and the preference ends with either A or U (T). According to neutral plot analysis, the correlation coefficient between the average value (GC12) of GC1, and GC2 and GC3 was 0.443. In addition, ENC-plot analysis also revealed that most genes cluster near the standard curve; besides, the corresponding analysis showed that the contribution rates of the 1-4 vector axes were 35.64%, 16.22%, 10.26% and 9.13%, respectively; and the GC content of the third synonymous codon (GC3s) and ENC were significantly positively correlated with the first vector axis (Axis1). In addition, the codon adaptation index (CAI) was negatively correlated with Axis1. Hence, it could finally be determined that CUA, AUA, GUU, UCU, CCC, ACA, GCU, CAU, AAA, GAA, UGA, CGC, GGC and GGA were optimal codons. Studying the optimization of the mitochondrial genome codon of Erinaceus amurensis using the molecular technique, the mechanism of drug action of its tissue could be explored further. [ABSTRACT FROM AUTHOR]