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The aim of this study was to establish a HPLC method for the simultaneous determination of baicalin and chlorogenic acid in Gongyingqinglan Heji. After extraction with 20% methanol, Gongyingqinglan Heji was separated by C18 chromatographic column (4.6 mmx150 mm, 3.5 µm). The column temperature was set at 35 °C, the detection wavelength was 324 nm, the photo-diode array detector was used for analysis, and external standard method was used for quantification. The results showed that the linear relationship between baicalin and chlorogenic acid was good within the ranges of 20.91~209.12 mg/L and 5.12~51.20 mg/L, respectively (R²>0.999 0). The average recovery rate of baicalin was 101.09%, and the coefficient of variation was 1.15%. The average recovery rate of chlorogenic acid was 99.59%, and the coefficient of variation was 1.46%. The system adaptability test was good, and the chromatographic column had good tolerance, fully meeting the detection requirements. The study indicates that this method has strong operability, with good stability, repeatability and accuracy, and is suitable for the simultaneous determination of baicalin and chlorogenic acid content in Gongyingqinglan Heji. [ABSTRACT FROM AUTHOR]

The study was to establish a method for the simultaneous determination of matrine and oxymatrine in Sanwei Quanshen Koufuye by HPLC. The test compares the detection effects of three amino-chromatographic columns (Agilent ZORBAX NH2, SHISEIDO CAPCELL PAK NH2, Waters SPHERSORB NH2). For separation effect, Agilent ZORBAX NH2 is preferred, but its running time is long. In order to save time and flow equivalent reagents, it was recommended to use SHISEIDO CAPCELL PAK NH2 column first and subselect Waters SPHERSORB NH2 column. After the absolute ethanol extraction, the amino chromatography column (4.6 mm x 250 mm, 5 μm) was used as the separation column, column temperature was 35 °C, and detection wavelength was 220 nm. Diode array detector was used for analysis, and external standard method was used for quantitative determination. The results showed that matrine and oxymatrine showed a good linear relationship ranging between 30 and 300 mg/L (R>0.999 0), and the average sample recovery rate was 97.64% with RSD 1.63%. The system adaptation test and the chromatography column tolerance was good, which fully meet the detection requirements. The study indicates that the method is easy to operate, with good repeatability, stability and accuracy, and can be used for the detection of matrine and oxymatrine contents in Sanwei Quanshen Koufuye. [ABSTRACT FROM AUTHOR]