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The experiment studied the effect of Arbuscular mycorrhizal fungi (AMF) function in the grasses and legumes mixture. A mixed sowing experiment of Medicago varia Martin. cv. Caoyuan No.3 and Agropyron mongolicum Keng. cv. Mengnong No. 1 was carried out on four years grassland. The paper researched AMF infection, soil nitrogen and phosphorus nutrients situation. The results showed that the total yield of mixed sowing was 24.3% higher than that of monoculture alfalfa and 46.9% higher than that of monoculture ice grass, and the plant height of mixed sowing ice grass was higher than that of monoculture ice grass. The pH value of rhizosphere soil of mixed grassland was significantly lower than that of non-rhizosphere soil. The vesicle infection rate of mixed sowing ice grass was significantly higher than that of monoculture ice grass. The total nitrogen of mixed sowing soil was higher than that of alfalfa monoculture or ice grass monoculture. The available P content in rhizosphere soil was higher than that in non-rhizosphere soil. The experiment indicates that the soil yield can be increased with the increase of Arbuscular fungi infection rate, the increase of nitrogen and phosphorus content in rhizosphere soil, and the decrease of soil pH value. [ABSTRACT FROM AUTHOR]

The experiment was to investigate the effect of arbuscular mycorrhizal fungus (AMF) inoculation under mixed saline alkali on the root configuration of alfalfa. In the experiment, Rhizophagus intraradices in AMF were inoculated with Medicago sativa variety WL168HQ, so that the two formed a plant-mycorrhizal joint repair system. Based on different salinity-alkali gradients, greenhouse potting experiments were carried out to study alfalfa root configurations with and without AMF inoculation under these conditions. The results showed that the total root length, bifurcation number, root tip number, surface area and volume of alfalfa after AMF inoculation were increased compared with the control group under the saline-free alkali (CK) treatment, and the total root length and bifurcation number were significantly different, while the average root diameter of alfalfa unvaccinated with AMF increased by 44.53% compared with the inoculation treatment. Salt stress, AMF and salt stress had significant effects on all root indexes of alfalfa, and the effect of salt stress on the average diameter, number of bifurcations, surface area and volume of alfalfa roots were more significant after inoculation with AMF under mixed saline conditions than on alkali stress. The experiment indicates that AMF vaccination is beneficial to the growth of alfalfa roots, helps alfalfa resist and adapt to salinity stress, thereby improving alfalfa quality and improving and treating land with mild saline. [ABSTRACT FROM AUTHOR]

The experiment was conducted to investigate the effect of dietary protein levels on growth performance and ruminal fungal flora structure of yaks. A total of 24 healthy two-year-old female yaks with an average body weight of (107.54±4.72) kg were randomly divided into four groups, with six replicates in each group and one yak in each replicate. Yaks in group L, group ML, group MH and group H were fed with were fed diets with similar ME and protein levels of 9.64%, 11.25%, 12.48% and 13.87%, respectively. The pre-test period lasted for 15 d and the formal test period lasted for 135 d. The results showed that the final weight and average daily gain of yaks in group MH and group H were significantly higher than those in group L and group ML (P<0.05). The higher protein level diets could increase the fungal richness. The dominant phyla of ruminal fungi in yak were Neocallimastigomycota, Basidiomycota and Ascomycota, and the relative abundance of Ascomycota increased obviously with the increase of dietary protein level. The dominant genera were Vishniacozyma and Cyllamyces. The experiment indicates that dietary CP level of 12.48% is more helpful to improve the growth performance of yaks, improve the richness of rumen fungal flora and regulate rumen microflora. [ABSTRACT FROM AUTHOR]