1. Evidence of oxidative stress in young and aged DJ-1-deficient mice
- Author
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Arjun V. Raman, Frederick P. Bellinger, Donato A. Di Monte, Vivian P. Chou, Jennifer Atienza-Duyanen, and Amy B. Manning-Boğ
- Subjects
Male ,DJ-1 ,Aging ,Parkinson's disease ,Protein Deglycase DJ-1 ,Chaperone ,medicine.disease_cause ,physiology [Mesencephalon] ,Biochemistry ,Synaptic Transmission ,Pathogenesis ,Mice ,Structural Biology ,Mesencephalon ,genetics [Oxidative Stress] ,metabolism [Neostriatum] ,Oncogene Proteins ,metabolism [Mesencephalon] ,Heat shock protein ,metabolism [Dopaminergic Neurons] ,Age Factors ,physiology [Aging] ,genetics [Aging] ,HSP60 ,Female ,genetics [Synaptic Transmission] ,Genetically modified mouse ,medicine.medical_specialty ,Biophysics ,Mice, Transgenic ,Biology ,Midbrain ,genetics [Brain Chemistry] ,Internal medicine ,Nigrostriatal ,Genetics ,medicine ,Animals ,ddc:610 ,metabolism [Aging] ,physiology [Dopaminergic Neurons] ,Molecular Biology ,Brain Chemistry ,PARK7 protein, mouse ,Dopaminergic Neurons ,Wild type ,genetics [Oncogene Proteins] ,Cell Biology ,Peroxiredoxins ,medicine.disease ,Neostriatum ,Oxidative Stress ,Endocrinology ,nervous system ,physiology [Synaptic Transmission] ,Parkinson’s disease ,Oxidative stress - Abstract
Loss of DJ-1 function contributes to pathogenesis in Parkinson’s disease. Here, we investigate the impact of aging and DJ-1 deficiency in transgenic mice. Ventral midbrain from young DJ-1-deficient mice revealed no change in 4-hydroxy-2-nonenal (4-HNE), but HSP60, HSP40 and striatal dopamine turnover were significantly elevated compared to wildtype. In aged mice, the chaperone response observed in wildtype animals was absent from DJ-1-deficient transgenics, and nigral 4-HNE immunoreactivity was enhanced. These changes were concomitant with increased striatal dopamine levels and uptake. Thus, increased oxidants and diminished protein quality control may contribute to nigral oxidative damage with aging in the model.
- Published
- 2013
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