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1. Escherichia coli engineered to produce eicosapentaenoic acid becomes resistant against oxidative damages

Author

Nishida, Takanori, Orikasa, Yoshitake, Yu, Reiko, Yamada, Akiko, Watanabe, Kazuo, and Okuyama, Hidetoshi

Subjects
Eicosapentaenoic acid, Viable but non-culturable, Oxidative stress, Hydrogen peroxide, Protein carbonylation
Abstract

ACP, acyl carrier protein; CFU, colony-forming unit; DH5αEPA+, E. coli DH5α transformant producing EPA; DH5αEPA−, E. coli DH5α transformant producing no EPA; DH5α(EPA−), E. coli DH5α carrying no vector; DHA, docosahexaenoic acid; EPA, eicosapentaenoic acid; FAME, fatty acid methyl ester; H2O2+, in the presence of H2O2; H2O2-, in the absence of H2O2; LB, Luria–Bertani; ORF, open reading frame; PUFA, polyunsaturated fatty acid; VBNC, viable but unculturable

Published
2006

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