1. A novel nucleotide receptor inXenopusactivates the cAMP second messenger pathway
- Author
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Timothy J. Geach, Andrea Townsend-Nicholson, Christelle Devader, Jacqui Tabler, Leslie Dale, and Christian M. Drew
- Subjects
P2Y receptor ,P2Y11 ,Xenopus ,Molecular Sequence Data ,Biophysics ,Xenopus Proteins ,Second Messenger Systems ,Biochemistry ,Homology (biology) ,Xenopus laevis ,Species Specificity ,Structural Biology ,cAMP ,Cyclic AMP ,Genetics ,Animals ,Humans ,Amino Acid Sequence ,Calcium Signaling ,Receptor ,Molecular Biology ,DNA Primers ,Synteny ,Mammals ,Base Sequence ,Sequence Homology, Amino Acid ,biology ,Phospholipase C ,Receptors, Purinergic P2 ,Gene Expression Regulation, Developmental ,Cell Biology ,biology.organism_classification ,Molecular biology ,Cell biology ,Gastrulation ,Embryo ,Second messenger system ,Calcium - Abstract
We describe a Xenopus P2Y receptor that shares only weak homology with members of the mammalian P2Y family, being most similar to human P2Y11. When activated by nucleotide analogs, it stimulates both calcium and cAMP mobilization pathways, a feature unique, among mammalian P2Y receptors, to P2Y11. Activity can be blocked by compounds known to act as antagonists of mammalian P2Y11. Genomic synteny between Xenopus and mammals suggests that the novel gene is a true ortholog of P2Y11. Xenopus P2Y11 is transcribed during embryonic development, beginning at gastrulation, and is enriched in the developing nervous system.
- Published
- 2007
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