1. The sequence of the hemoregulatory peptide is present in Gi alpha proteins
- Author
-
Stein Frostad, Dietmar Kamp, Hege I. Tøn, and Ole Didrik Laerum
- Subjects
G-protein ,G protein ,Molecular Sequence Data ,Biophysics ,Peptide ,Biology ,In Vitro Techniques ,Biochemistry ,Mice ,Structure-Activity Relationship ,GTP-binding protein regulators ,Structural Biology ,GTP-Binding Proteins ,Genetics ,Animals ,Amino Acid Sequence ,Hemoregulatory peptide ,Molecular Biology ,Peptide sequence ,chemistry.chemical_classification ,Oligopeptide ,Effector ,Cell Biology ,Hematopoiesis ,Pyrrolidonecarboxylic Acid ,chemistry ,Hemopoiesis ,Signal transduction ,Sequence motif ,Oligopeptides - Abstract
The hemoregulatory peptide PyroGlu-Glu-Asp-Cys-Lys (HP5b), which inhibits myelopoietic colony formation in vitro, is shown to be a sequence motif which is also part of the effector domain of Gi alpha proteins. Out of 8 synthetic peptides with sequence variations of HP5b, those with the closest similarity to the Gi alpha sequence are biologically active. The inhibitory effect appears to be dependent on the blocked N-terminus. It is postulated that these peptides may interfere with signal transduction mediated by Gi alpha proteins.
- Published
- 1990