1. Monoclonal antibodies to lipocortin-1 as probes for biological function.
- Author
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Pepinsky RB, Sinclair LK, Dougas I, Liang CM, Lawton P, and Browning JL
- Subjects
- Animals, Annexins, Antibodies, Monoclonal biosynthesis, Antibody Specificity, Blotting, Western, Calcium-Binding Proteins pharmacology, Cyanogen Bromide, Enzyme-Linked Immunosorbent Assay, Epitopes immunology, Female, Humans, Indicators and Reagents, Mice, Mice, Inbred BALB C, Peptide Fragments immunology, Peptide Mapping, Phospholipases A antagonists & inhibitors, Phospholipases A2, Recombinant Proteins, Antibodies, Monoclonal immunology, Calcium-Binding Proteins immunology
- Abstract
We have developed two monoclonal antibodies to human lipocortin-1 (103 and 105) as reagents for quantitating the protein in biological systems and neutralizing its activity. Lipo 105 is a high affinity antibody that is functional in ELISA and Western blot formats. The antibody recognizes a site between amino acids 30 and 55 in the lipocortin-1 sequence and can be used on native or denatured protein. Lipo 103 is an antibody that neutralizes the phospholipase A2 inhibitory activity of lipocortin-1 by blocking binding of the protein to phospholipid surfaces. The antibody is specific for native human lipocortin-1. Lipo 103 was recently shown to block lipocortin-1-dependent differentiation of a squamous carcinoma cell line, demonstrating its usefulness as a probe for function.
- Published
- 1990
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