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Your search keyword '"Sakura Azuma"' showing total 2 results
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2 results on '"Sakura Azuma"'

1. Expression screening of 17q12-21 amplicon reveals GRB7 as an ERBB2-dependent oncogene

Author

Hirokazu Ohbayashi, Naoki Goshima, Hiromi Mochizuki, Jiro Fujimoto, Kosuke Ishikawa, Shinya Watanabe, Atsuka Matsui, Ayano Doi, Makoto Saito, Yoshifumi Kawamura, Sakura Azuma, Yuka Yanagisawa, Reiko Honma, Kentaro Kumazawa, Emi Ito, Kentaro Semba, Shiori Takebe, Takaomi Ishida, Yukiko Kato, and Jun-ichi Imai

Subjects
Receptor, ErbB-2, Biophysics, Down-Regulation, medicine.disease_cause, Biochemistry, Mice, Structural Biology, Complementary DNA, Gene duplication, Proto-Oncogenes, Genetics, medicine, Animals, Humans, Point Mutation, GRB7, Phosphorylation, ERBB2, Molecular Biology, Gene, Sequence Deletion, Oncogene, biology, Gene Amplification, Cell Biology, Amplicon, Expression screening, Recombinant Proteins, Protein Structure, Tertiary, Cell Transformation, Neoplastic, Mutagenesis, GRB7 Adaptor Protein, Tumorigenesis, biology.protein, NIH 3T3 Cells, Signal transduction, Carcinogenesis, Protein Processing, Post-Translational, Proto-Oncogene Proteins c-akt, Chromosomes, Human, Pair 17, Signal Transduction
Abstract

Gene amplification is a major genetic alteration in human cancers. Amplicons, amplified genomic regions, are believed to contain “driver” genes responsible for tumorigenesis. However, the significance of co-amplified genes has not been extensively studied. We have established an integrated analysis system of amplicons using retrovirus-mediated gene transfer coupled with a human full-length cDNA set. Applying this system to 17q12–21 amplicon observed in breast cancer, we identified GRB7 as a context-dependent oncogene, which modulates the ERBB2 signaling pathway through enhanced phosphorylation of ERBB2 and Akt. Our work provides an insight into the biological significance of gene amplification in human cancers.

Published
2012

2. Novel clusters of highly expressed genes accompany genomic amplification in breast cancers

Author

Shinya Watanabe, Reiko Honma, Jun-ichiro Inoue, Nobuo Nomura, Tetsunari Oyama, Emi Ito, Susumu Ohwada, Sakura Azuma, Yuka Yanagisawa, Tetsu Akiyama, Jun-ichi Imai, and Kentaro Semba

Subjects
Biophysics, Breast Neoplasms, Biology, Microarray, Biochemistry, Breast cancer, Structural Biology, Cell Line, Tumor, Gene duplication, Genetics, medicine, Chromosomes, Human, Humans, Molecular Biology, Gene, Southern blot, Oligonucleotide Array Sequence Analysis, Gene amplification, Gene Expression Profiling, Cancer, Cell Biology, Amplicon, medicine.disease, Neoplasm Proteins, Gene expression profiling, Gene Expression Regulation, Neoplastic, Phenotype, Multigene Family, Female, Comparative genomic hybridization
Abstract

Breast cancer is the most common cancer in women worldwide. To identify novel amplicons involved in the mammary carcinogenesis, we constructed gene expression maps of chromosomes in 35 human breast cancer cell lines and extracted six candidate amplicons containing highly expressed gene clusters on chromosomes 8, 17, and X. We also confirmed the presence of the identified amplicons in clinical specimens by Southern blot analysis. Highly expressed genes identified in the amplicons will contribute to the characterization of breast cancer phenotypes, thereby providing novel targets for anticancer therapies.

Published
2007

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