1. Localisation and regulation of the eIF4E-binding protein 4E-BP3
- Author
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Kleijn, Miranda, Scheper, Gert C., Wilson, Mary L., Tee, Andrew R., and Proud, Christopher G.
- Subjects
- *
CARRIER proteins , *T cells - Abstract
The cap-binding protein eIF4E-binding protein 3 (4E-BP3) was identified some years ago, but its properties have not been investigated in detail. In this report, we investigated the regulation and localisation of 4E-BP3. We show that 4E-BP3 is present in the nucleus as well as in the cytoplasm in primary T cells, HEK293 cells and HeLa cells. 4E-BP3 was associated with eIF4E in both cell compartments. Furthermore, 4E-BP3/eIF4E association in the cytoplasm was regulated by serum or interleukin-2 starvation in the different cell types. Rapamycin did not affect the association of eIF4E with 4E-BP3 in the cytoplasm or in the nucleus. [Copyright &y& Elsevier]
- Published
- 2002
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