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2. Protein kinase C translocation in relation to proliferative state of C3H 10T1/2 cells.

Author

Miłoszewska J, Trawicki W, Janik P, Moraczewski J, Przybyszewska M, and Szaniawska B

Subjects
Animals, Cell Division, Cell Line, Cell Membrane enzymology, Cytosol enzymology, Fibroblasts enzymology, Mice, Mice, Inbred C3H, Osmolar Concentration, Sodium Chloride, Fibroblasts cytology, Protein Kinase C metabolism
Abstract

Protein kinase C (PKC) activity of cytosol and membrane fractions of 10T1/2 cells was studied. In cytosol of fast growing cells PKC activity was found in material eluted with 0.150 M NaCl whereas in membrane fractions activity was eluted with 0.065 and 0.150 M NaCl. In the membrane fraction of confluent cells, in contrast to cytosol, very low PKC activity was observed. The translocation pattern of PKC activity eluted with 0.065 M NaCl may be associated with proliferation.

Published
1986
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