Search

Your search keyword '"Michel A"' showing total 2,782 results
Start Over Author "Michel A" Journal febs letters
2,782 results on '"Michel A"'

1. Plant cytokinesis and the construction of new cell wall

Author

Rosalie Sinclair, Grace Hsu, Destiny Davis, Mingqin Chang, Michel Rosquete, Janet H. Iwasa, and Georgia Drakakaki

Subjects
Cytoplasm, Cell Wall, Polysaccharides, Structural Biology, Plant Cells, Genetics, Biophysics, Cell Biology, Plants, Molecular Biology, Biochemistry, Cytokinesis
Abstract

Cytokinesis in plants is fundamentally different from that in animals and fungi. In plant cells, a cell plate forms through the fusion of cytokinetic vesicles and then develops into the new cell wall, partitioning the cytoplasm of the dividing cell. The formation of the cell plate entails multiple stages that involve highly orchestrated vesicle accumulation, fusion and membrane maturation, which occur concurrently with the timely deposition of polysaccharides such as callose, cellulose and cross-linking glycans. This review summarizes the major stages in cytokinesis, endomembrane components involved in cell plate assembly and its transition to a new cell wall. An animation that can be widely used for educational purposes further summarizes the process.

Published
2022

3. Homodimerization of coronin A through the C‐terminal coiled‐coil domain is essential for multicellular differentiation ofDictyostelium discoideum

Author

Vera Studer, Thomas Fiedler, Michel O. Steinmetz, Timothy Sharpe, Tohnyui Ndinyanka Fabrice, Lenka Faltova, Adrien F. Vinet, Jean Pieters, and Richard A. Kammerer

Subjects
Coiled coil, 0303 health sciences, 030302 biochemistry & molecular biology, Biophysics, Coronin, Cell Biology, Biology, biology.organism_classification, Biochemistry, Dictyostelium, Dictyostelium discoideum, Domain (software engineering), Cell biology, 03 medical and health sciences, Multicellular organism, Wd repeat, Structural Biology, Genetics, biology.protein, Molecular Biology, Actin, 030304 developmental biology
Abstract

Coronin proteins are widely expressed among eukaryotic organisms. Most coronins consist of a WD repeat domain followed by a C-terminal coiled coil. Dictyostelium discoideum expresses a single short coronin coronin A, which has been implicated in both actin modulation as well as multicellular differentiation. Whether coronin A's coiled coil is important for functionality, as well as the oligomeric state of coronin A is not known. Here, we show that the coiled-coil domain in Dictyostelium coronin A functions in homodimerization, is dispensable for coronin A stability and localization but essential for multicellular differentiation. These results allow a better understanding of the role for the coiled-coil domain of coronin A in oligomerization and demonstrate that its presence is essential for multicellular differentiation.

Published
2020

5. Hirudin or hirudin‐like factor ‐ that is the question: insights from the analyses of natural and synthetic HLF variants

Author

Michel Böhmert, Phil Lukas, Jan-Peter Hildebrandt, and Christian Müller

Subjects
Biophysics, Hirudin, Computational biology, Protein Engineering, Biochemistry, 03 medical and health sciences, Protein Domains, Structural Biology, Leeches, Genetics, medicine, Animals, Humans, Saliva, Mode of action, Molecular Biology, 030304 developmental biology, 0303 health sciences, biology, Chemistry, 030302 biochemistry & molecular biology, Thrombin, Cell Biology, Hirudins, biology.organism_classification, N-terminus, Hirudo medicinalis, Domain (ring theory), Mutagenesis, Site-Directed, medicine.drug
Abstract

The hirudin-like factor 1 (HLF1) of Hirudo medicinalis belongs to a new class of leech-derived factors. In previous investigations, HLF1 did not exhibit anticoagulatory activities. Here, we describe the analysis of natural and synthetic variants of HLF1 and HLF-Hyb, a yet uncharacterized member of the HLF family. Modifications within the N terminus of HLF1 have a strong impact on its activity. Some variants of HLF1 exhibit thrombin-inhibiting activity comparable to hirudins, whereas others have reduced or no activity. The analyses of HLF-Hyb variants revealed a strong impact of the central globular domain on activity. Our results indicate a comparable mode of action of hirudins and thrombin-inhibiting HLF variants. Finally, we propose and discuss criteria for classifying hirudins and HLFs.

Published
2019

6. Tau filaments in neurodegenerative diseases

Author

Michel Goedert

Subjects
0301 basic medicine, Tau protein, Biophysics, tau Proteins, macromolecular substances, Biology, Protein Aggregation, Pathological, Biochemistry, Protein Aggregates, 03 medical and health sciences, 0302 clinical medicine, Alzheimer Disease, Structural Biology, mental disorders, Genetics, medicine, Animals, Humans, Protein Isoforms, Dementia, Molecular Biology, Neurodegeneration, Brain, Neurodegenerative Diseases, Cell Biology, Human brain, medicine.disease, Tau isoforms, 030104 developmental biology, medicine.anatomical_structure, biology.protein, Neuroscience, 030217 neurology & neurosurgery, Frontotemporal dementia
Abstract

The ordered assembly of Tau protein into abnormal filamentous inclusions is a defining characteristic of many human neurodegenerative diseases. Thirty years ago, we reported that Tau is an integral component of the intraneuronal filaments of Alzheimer's disease. All six brain Tau isoforms make up those filaments. Twenty years ago, we and others showed that mutations in MAPT, the Tau gene, cause familial forms of frontotemporal dementia, thus proving that dysfunction of Tau protein is sufficient to cause neurodegeneration and dementia. More recently, we showed that high-resolution structures of Tau filaments from human brain can be determined by electron cryo-microscopy. These filaments may form the seeds that underlie the prion-like properties of aggregated tau.

Published
2018

9. Genomic Exploration of the Hemiascomycetous Yeasts: 21. Comparative functional classification of genes

Author

Gaillardin, Claude, Duchateau-Nguyen, Guillemette, Tekaia, Fredj, Llorente, Bertrand, Casaregola, Serge, Toffano-Nioche, Claire, Aigle, Michel, Artiguenave, François, Blandin, Gaëlle, Bolotin-Fukuhara, Monique, Bon, Elisabeth, Brottier, Philippe, de Montigny, Jacky, Dujon, Bernard, Durrens, Pascal, Lépingle, Andrée, Malpertuy, Alain, Neuvéglise, Cécile, Ozier-Kalogéropoulos, Odile, Potier, Serge, Saurin, William, Termier, Michel, Wésolowski-Louvel, Micheline, Wincker, Patrick, Souciet, Jean-Luc, and Weissenbach, Jean

Published
2000
Full Text
View/download PDF

10. EWS‐FLI1 impairs aryl hydrocarbon receptor activation by blocking tryptophan breakdown via the kynurenine pathway

Author

Mutz, Cornelia N, Schwentner, Raphaela, Kauer, Maximilian O, Katschnig, Anna M, Kromp, Florian, Aryee, Dave N T, Erhardt, Sophie, Goiny, Michel, Alonso, Javier, Fuchs, Dietmar, Kovar, Heinrich, Austrian Science Fund, 7º Programa Marco - Comisión Europea, Asociación Pablo Ugarte and Miguelañez S.A, ASION‐La Hucha de Tomás, Fundación La Sonrisa de Alex, Instituto de Salud Carlos III, Red Temática de Investigación Cooperativa en Cáncer (España), Swedish Medical Research Council, Unión Europea. Comisión Europea. 7 Programa Marco, Asociación Pablo Ugarte contra el cáncer infantil, Asociación Infantil Oncológica de Madrid. La hucha de Tomás, Fundación la Sonrisa de Alex para la investigación y el tratamiento del sarcoma de Ewing, and Miguelañez

Subjects
Oncogene Proteins, Fusion, aryl hydrocarbon receptor, Proto-Oncogene Protein c-fli-1, fungi, Tryptophan, Research Letters, Tryptophan Oxygenase, Cell Line, Autocrine Communication, EWS‐FLI1, Receptors, Aryl Hydrocarbon, Metabolism (Editor's Choice), Research Letter, Humans, RNA-Binding Protein EWS, EWS-FLI1, Aryl hydrocarbon receptor, Kynurenine, Signal Transduction
Abstract

Ewing sarcoma (ES) is an aggressive pediatric tumor driven by the fusion protein EWS-FLI1. We report that EWS-FLI1 suppresses TDO2-mediated tryptophan (TRP) breakdown in ES cells. Gene expression and metabolite analyses reveal an EWS-FLI1-dependent regulation of TRP metabolism. TRP consumption increased in the absence of EWS-FLI1, resulting in kynurenine and kynurenic acid accumulation, both aryl hydrocarbon receptor (AHR) ligands. Activated AHR binds to the promoter region of target genes. We demonstrate that EWS-FLI1 knockdown results in AHR nuclear translocation and activation. Our data suggest that EWS-FLI1 suppresses autocrine AHR signaling by inhibiting TDO2-catalyzed TRP breakdown. The authors thank Dr. Peter Münzel for providing the pT81/3xDRE plasmid. We also thank Dr. Andreas Heitger posthumously for critical discussion and scientific advice. Austrian Science Fund (FWF), ERA‐NET TransCan project PROVABES, project number I 1225‐B19; European Union's FP7 project ASSET (grant agreement No. 259348). J.A. is supported by Asociación Pablo Ugarte and Miguelañez S.A, ASION‐La Hucha de Tomás, Fundación La Sonrisa de Alex and Instituto de Salud Carlos III (PI12/00816 and Spanish Cancer Network RTICC RD12/0036/0027); the Swedish Medical Research Council (2009‐7053; 2013‐2838). Sí

Published
2016

17. Genomic Exploration of the Hemiascomycetous Yeasts: 3. Methods and strategies used for sequence analysis and annotation

Author

Tekaia, Fredj, Blandin, Gaëlle, Malpertuy, Alain, Llorente, Bertrand, Durrens, Pascal, Toffano-Nioche, Claire, Ozier-Kalogeropoulos, Odile, Bon, Elisabeth, Gaillardin, Claude, Aigle, Michel, Bolotin-Fukuhara, Monique, Casarégola, Serge, de Montigny, Jacky, Lépingle, Andrée, Neuvéglise, Cécile, Potier, Serge, Souciet, Jean-Luc, Wésolowski-Louvel, Micheline, and Dujon, Bernard

Published
2000
Full Text
View/download PDF

18. Genomic Exploration of the Hemiascomycetous Yeasts: 19. Ascomycetes-specific genes

Author

Malpertuy, Alain, Tekaia, Fredj, Casarégola, Serge, Aigle, Michel, Artiguenave, Francois, Blandin, Gaëlle, Bolotin-Fukuhara, Monique, Bon, Elisabeth, Brottier, Philippe, de Montigny, Jacky, Durrens, Pascal, Gaillardin, Claude, Lépingle, Andrée, Llorente, Bertrand, Neuvéglise, Cécile, Ozier-Kalogeropoulos, Odile, Potier, Serge, Saurin, William, Toffano-Nioche, Claire, Wésolowski-Louvel, Micheline, Wincker, Patrick, Weissenbach, Jean, Souciet, Jean-Luc, and Dujon, Bernard

Published
2000
Full Text
View/download PDF

19. Genomic Exploration of the Hemiascomycetous Yeasts: 20. Evolution of gene redundancy compared to Saccharomyces cerevisiae

Author

Llorente, Bertrand, Durrens, Pascal, Malpertuy, Alain, Aigle, Michel, Artiguenave, François, Blandin, Gaëlle, Bolotin-Fukuhara, Monique, Bon, Elisabeth, Brottier, Philippe, Casaregola, Serge, Dujon, Bernard, de Montigny, Jacky, Lépingle, Andrée, Neuvéglise, Cécile, Ozier-Kalogeropoulos, Odile, Potier, Serge, Saurin, William, Tekaia, Fredj, Toffano-Nioche, Claire, Wésolowski-Louvel, Micheline, Wincker, Patrick, Weissenbach, Jean, Souciet, Jean-Luc, and Gaillardin, Claude

Published
2000
Full Text
View/download PDF

20. Genomic Exploration of the Hemiascomycetous Yeasts: 4. The genome of Saccharomyces cerevisiae revisited

Author

Blandin, Gaëlle, Durrens, Pascal, Tekaia, Fredj, Aigle, Michel, Bolotin-Fukuhara, Monique, Bon, Elisabeth, Casarégola, Serge, de Montigny, Jacky, Gaillardin, Claude, Lépingle, Andrée, Llorente, Bertrand, Malpertuy, Alain, Neuvéglise, Cécile, Ozier-Kalogeropoulos, Odile, Perrin, Arnaud, Potier, Serge, Souciet, Jean-Luc, Talla, Emmanuel, Toffano-Nioche, Claire, Wésolowski-Louvel, Micheline, Marck, Christian, and Dujon, Bernard

Published
2000
Full Text
View/download PDF

21. Genomic Exploration of the Hemiascomycetous Yeasts: 1. A set of yeast species for molecular evolution studies

Author

Souciet, Jean-Luc, Aigle, Michel, Artiguenave, François, Blandin, Gaëlle, Bolotin-Fukuhara, Monique, Bon, Elisabeth, Brottier, Philippe, Casaregola, Serge, de Montigny, Jacky, Dujon, Bernard, Durrens, Pascal, Gaillardin, Claude, Lépingle, Andrée, Llorente, Bertrand, Malpertuy, Alain, Neuvéglise, Cécile, Ozier-Kalogéropoulos, Odile, Potier, Serge, Saurin, William, Tekaia, Fredj, Toffano-Nioche, Claire, Wésolowski-Louvel, Micheline, Wincker, Patrick, and Weissenbach, Jean

Published
2000
Full Text
View/download PDF

22. Genomic Exploration of the Hemiascomycetous Yeasts: 18. Comparative analysis of chromosome maps and synteny with Saccharomyces cerevisiae

Author

Llorente, Bertrand, Malpertuy, Alain, Neuvéglise, Cécile, de Montigny, Jacky, Aigle, Michel, Artiguenave, François, Blandin, Gaëlle, Bolotin-Fukuhara, Monique, Bon, Elisabeth, Brottier, Philippe, Casaregola, Serge, Durrens, Pascal, Gaillardin, Claude, Lépingle, Andrée, Ozier-Kalogéropoulos, Odile, Potier, Serge, Saurin, William, Tekaia, Fredj, Toffano-Nioche, Claire, Wésolowski-Louvel, Micheline, Wincker, Patrick, Weissenbach, Jean, Souciet, Jean-Luc, and Dujon, Bernard

Published
2000
Full Text
View/download PDF

43. The lipid peroxidation end-product 4-HNE induces COX-2 expression through p38MAPK activation in 3T3-L1 adipose cell

Author

Alain Géloën, Michel Lagarde, A.F. Soares, Michel Guichardant, and Bader Zarrouki

Subjects
medicine.medical_specialty, Biophysics, Adipose tissue, Inflammation, Cysteine Proteinase Inhibitors, Dinoprost, medicine.disease_cause, p38 Mitogen-Activated Protein Kinases, Biochemistry, Dinoprostone, Gene Expression Regulation, Enzymologic, Lipid peroxidation, Mice, chemistry.chemical_compound, Structural Biology, Adipocyte, Internal medicine, Adipocytes, Genetics, medicine, Animals, RNA, Messenger, Molecular Biology, 3T3-L1, Aldehydes, biology, 3T3 Cells, Cell Biology, Cyclooxygenase, Enzyme Activation, Oxidative Stress, Endocrinology, chemistry, Cyclooxygenase 2, Prostaglandin-Endoperoxide Synthases, p38MAPK, biology.protein, 4-Hydroxy-2-nonenal, Phosphorylation, Lipid Peroxidation, medicine.symptom, Oxidative stress
Abstract

Oxidative stress and low grade chronic inflammation are increased in accumulating fat. Our objective was to test whether 4-hydroxynonenal (4-HNE), an end-product of lipid peroxidation, affects cyclooxygenases in 3T3-L1 adipose cells. 4-HNE increased COX-2 mRNA and protein expression and p38MAP-kinase phosphorylation in a dose-dependent manner. Pretreatment of 3T3-L1 cells by a selective inhibitor of p38MAPK (PD 169316) abolished 4-HNE and glucose oxidase induced COX-2 expression.Our results show that oxidative stress induces COX-2 expression through the production of 4-HNE which activates p38MAPKinase, suggesting that 4-HNE links oxidative stress and chronic inflammation through the activation of cyclooxygenase.

Published
2007

44. Hirudin or hirudin‐like factor ‐ that is the question: insights from the analyses of natural and synthetic HLF variants.

Author

Müller, Christian, Lukas, Phil, Böhmert, Michel, and Hildebrandt, Jan‐Peter

Subjects
BLOOD coagulation, INVESTIGATIONS
Abstract

The hirudin‐like factor 1 (HLF1) of Hirudo medicinalis belongs to a new class of leech‐derived factors. In previous investigations, HLF1 did not exhibit anticoagulatory activities. Here, we describe the analysis of natural and synthetic variants of HLF1 and HLF‐Hyb, a yet uncharacterized member of the HLF family. Modifications within the N terminus of HLF1 have a strong impact on its activity. Some variants of HLF1 exhibit thrombin‐inhibiting activity comparable to hirudins, whereas others have reduced or no activity. The analyses of HLF‐Hyb variants revealed a strong impact of the central globular domain on activity. Our results indicate a comparable mode of action of hirudins and thrombin‐inhibiting HLF variants. Finally, we propose and discuss criteria for classifying hirudins and HLFs. [ABSTRACT FROM AUTHOR]

Published
2020
Full Text
View/download PDF

45. Reduced phosphatase activity of SHP-2 in LEOPARD syndrome: Consequences for PI3K binding on Gab1

Author

Alexandra Montagner, Patrick Raynal, Nadine Hanna, Béatrice Parfait, Maria A. Miteva, Michel Vidal, Wen Hwa Lee, Michel Vidaud, Centre d’Etudes et de Recherches en Psychopathologie et Psychologie de la Santé (CERPPS), and Université Toulouse - Jean Jaurès (UT2J)

Subjects
Models, Molecular, animal structures, [SDV]Life Sciences [q-bio], Mutant, Phosphatase, Biophysics, Protein Tyrosine Phosphatase, Non-Receptor Type 11, Protein tyrosine phosphatase, PTPN11, Biology, medicine.disease_cause, PI3K, Biochemistry, LEOPARD Syndrome, Catalysis, Phosphatidylinositol 3-Kinases, 03 medical and health sciences, 0302 clinical medicine, Structural Biology, Epidermal growth factor, Chlorocebus aethiops, Genetics, medicine, Animals, Vero Cells, LEOPARD syndrome, SHP-2 phosphatase activity, Molecular Biology, ComputingMilieux_MISCELLANEOUS, Adaptor Proteins, Signal Transducing, 030304 developmental biology, Gab1, 0303 health sciences, Mutation, Epidermal Growth Factor, Intracellular Signaling Peptides and Proteins, Wild type, Cell Biology, Molecular biology, 3. Good health, 030220 oncology & carcinogenesis, embryonic structures, Protein Tyrosine Phosphatases, Protein Binding
Abstract

LEOPARD (LS) and Noonan (NS) are overlapping syndromes associated with distinct mutations of SHP-2. Whereas NS mutations enhance SHP-2 catalytic activity, we show that the activity of three representative LS mutants is undetectable when assayed using a standard protein tyrosine phosphatase (PTP) substrate. A different assay using a specific SHP-2 substrate confirms their decreased PTP activity, but also reveals a significant activity of the T468M mutant. In transfected cells stimulated with epidermal growth factor, the least active LS mutants promote Gab1/PI3K binding, validating our in vitro data. LS mutants thus display a reduced PTP activity both in vitro and in transfected cells.

Published
2006

Catalog

Books, media, physical & digital resources
See catalog results