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Start Over Descriptor "MiR-25" Journal febs letters
2 results on '"MiR-25"'

1. miR‐25 alleviates polyQ‐mediated cytotoxicity by silencing ATXN3.

Author

Huang, Fengzhen, Zhang, Li, Long, Zhe, Chen, Zhao, Hou, Xuan, Wang, Chunrong, Peng, Huirong, Wang, Junling, Li, Jiada, Duan, Ranhui, Xia, Kun, Chuang, De-Maw, Tang, Beisha, and Jiang, Hong

Abstract

MicroRNAs (miRNAs) have been reported to play significant roles in the pathogenesis of various polyQ diseases. This study aims to investigate the regulation of ATXN3 gene expression by miRNA. We found that miR‐25 reduced both wild‐type and polyQ‐expanded mutant ataxin‐3 protein levels by interacting with the 3′UTR of ATXN3 mRNA. miR‐25 also increased cell viability, decreased early apoptosis, and downregulated the accumulation of mutant ataxin‐3 protein aggregates in SCA3/MJD cells. These novel results shed light on the potential role of miR‐25 in the pathogenesis of SCA3/MJD, and provide a possible therapeutic intervention for this disorder.ATXN3 is a target of miR‐25. miR‐25 reduces ATXN3 protein levels. miR‐25 decreases polyQ‐mediated toxicity and NII formation. [ABSTRACT FROM AUTHOR]

Published
2014
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2. miR-25 alleviates polyQ-mediated cytotoxicity by silencing ATXN3

Author

Xuan Hou, Junling Wang, Kun Xia, Li Zhang, Hong Jiang, Zhe Long, Zhao Chen, Fengzhen Huang, Ranhui Duan, Beisha Tang, Huirong Peng, Chunrong Wang, Jia-Da Li, and De-Maw Chuang

Subjects
Neuronal intranuclear inclusion, congenital, hereditary, and neonatal diseases and abnormalities, Cell Survival, Cytotoxicity, miR-25, Mutant, Biophysics, Nerve Tissue Proteins, Biology, Biochemistry, Article, Structural Biology, Gene expression, microRNA, Genetics, Gene silencing, Humans, Viability assay, Gene Silencing, Ataxin-3, Molecular Biology, 3' Untranslated Regions, Gene expression regulation, Regulation of gene expression, Messenger RNA, Base Sequence, Cytotoxins, ATXN3, Nuclear Proteins, Cell Biology, Machado-Joseph Disease, Molecular biology, Cell biology, Repressor Proteins, MicroRNAs, HEK293 Cells, Apoptosis, Mutation, Peptides, Spinocerebellar ataxia type 3/Machado–Joseph disease
Abstract

MicroRNAs (miRNAs) have been reported to play significant roles in the pathogenesis of various polyQ diseases. This study aims to investigate the regulation of ATXN3 gene expression by miRNA. We found that miR-25 reduced both wild-type and polyQ-expanded mutant ataxin-3 protein levels by interacting with the 3′UTR of ATXN3 mRNA. miR-25 also increased cell viability, decreased early apoptosis, and downregulated the accumulation of mutant ataxin-3 protein aggregates in SCA3/MJD cells. These novel results shed light on the potential role of miR-25 in the pathogenesis of SCA3/MJD, and provide a possible therapeutic intervention for this disorder.

Published
2014

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