1. Structure, chromosome localization, and tissue distribution of the mouse twik K+ channel gene
- Author
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Isabelle Arrighi, Florian Lesage, Jacques Barhanin, Georges F. Carle, Jean-Claude Scimeca, Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Génétique, physiopathologie et ingénierie du tissu osseux (GéPITOS), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)
- Subjects
Potassium Channels ,Transcription, Genetic ,Gene Expression ,MESH: Base Sequence ,Biochemistry ,Mice ,Exon ,0302 clinical medicine ,Structural Biology ,[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] ,Coding region ,Tissue Distribution ,MESH: Animals ,Peptide Chain Initiation, Translational ,Genomic organization ,0303 health sciences ,Chromosome Mapping ,MESH: Potassium Channels, Tandem Pore Domain ,MESH: Potassium Channels ,Potassium channel ,[SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system ,Background conductance ,MESH: Peptide Chain Initiation, Translational ,DNA, Complementary ,MESH: Gene Expression ,Molecular Sequence Data ,Biophysics ,Mouse chromosome 8 ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,Development ,Biology ,03 medical and health sciences ,Potassium Channels, Tandem Pore Domain ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Genetics ,Animals ,RNA, Messenger ,MESH: Tissue Distribution ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] ,[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials ,MESH: Mice ,Molecular Biology ,Gene ,MESH: RNA, Messenger ,030304 developmental biology ,Binding Sites ,MESH: Molecular Sequence Data ,Base Sequence ,Chromosome localization ,MESH: Transcription, Genetic ,Embryogenesis ,Chromosome ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,MESH: DNA, Complementary ,Cell Biology ,Molecular biology ,MESH: Binding Sites ,MESH: Chromosome Mapping ,030217 neurology & neurosurgery - Abstract
International audience; We have recently discovered a new class of potassium channels with two pore-forming domains and four membrane-spanning domains. When heterologously expressed, these channels produce time- and voltage-independent currents that classify them as background or leak channels. TWIK (for tandem of P domains in a weak inwardly rectifying K+ channel) was the first member of this family to be cloned. Here, we describe the genomic organization of TWIK in the mouse. The coding sequence as well as the untranslated sequences are contained in three exons. The twik gene (or KCNK1) has been mapped to chromosome 8, consistent with its localization to 1q42-43 in human. The twik gene is expressed in virtually all mouse tissues. It is most abundantly expressed in brain and moderately in other organs such as kidney. The level of expression is increased in brain and kidney from neonate to adult animals, but the TWIK message is also detected during embryogenesis, as early as day 7 post conception.
- Published
- 1998