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3 results on '"Kokabu S"'

1. Netrin-4 derived from murine vascular endothelial cells inhibits osteoclast differentiation in vitro and prevents bone loss in vivo.

Author

Enoki Y, Sato T, Tanaka S, Iwata T, Usui M, Takeda S, Kokabu S, Matsumoto M, Okubo M, Nakashima K, Yamato M, Okano T, Fukuda T, Chida D, Imai Y, Yasuda H, Nishihara T, Akita M, Oda H, Okazaki Y, Suda T, and Yoda T

Subjects
Animals, Bone Resorption metabolism, Cells, Cultured, Coculture Techniques, Endothelium, Vascular pathology, Macrophages metabolism, Mice, Mice, Inbred C57BL, Netrins, Osteoporosis chemically induced, RANK Ligand, Cell Differentiation, Endothelial Cells metabolism, Nerve Growth Factors physiology, Osteoclasts physiology, Osteoporosis metabolism
Abstract

Bone is a highly vascularized organ, thus angiogenesis is a vital process during bone remodeling. However, the role of vascular systems in bone remodeling is not well recognized. Here we show that netrin-4 inhibits osteoclast differentiation in vitro and in vivo. Co-cultures of bone marrow macrophages with vascular endothelial cells markedly inhibited osteoclast differentiation. Adding a neutralizing antibody, or RNA interference against netrin-4, restored in vitro osteoclast differentiation. Administration of netrin-4 prevented bone loss in an osteoporosis mouse model by decreasing the osteoclast number. We propose that vascular endothelial cells interact with bone in suppressing bone through netrin-4., (Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)

Published
2014
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2. Expression of TLE3 by bone marrow stromal cells is regulated by canonical Wnt signaling.

Author

Kokabu S, Sato T, Ohte S, Enoki Y, Okubo M, Hayashi N, Nojima J, Tsukamoto S, Fukushima Y, Sakata Y, Katagiri T, Rosen V, and Yoda T

Subjects
Animals, Base Sequence, Binding Sites, Cell Differentiation, Cell Line, Conserved Sequence, Genomics, Humans, Mice, Molecular Sequence Data, Osteoblasts cytology, Response Elements genetics, beta Catenin metabolism, Co-Repressor Proteins genetics, Gene Expression Regulation, Mesenchymal Stem Cells cytology, Mesenchymal Stem Cells metabolism, Signal Transduction, Wnt Proteins metabolism
Abstract

Transducing-like enhancer of split 3 (TLE3), one of the Groucho/TLE family members, targets Runx2 transcription and suppresses osteoblast differentiation in bone marrow stromal cells (BMSCs). Here, we identify Wnt responsive elements of the TLE3 promoter region through comparative genomic and functional analyses and show that expression of TLE3 is increased by Wnt signaling, which is important for osteoblast differentiation. We also demonstrated that TLE3 is able to suppress canonical Wnt signaling in BMSCs. Taken together, our data suggest that induction of TLE3 by Wnt signaling is part of a negative feedback loop active during osteoblast differentiation., (Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)

Published
2014
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3. Functional role of acetylcholine and the expression of cholinergic receptors and components in osteoblasts.

Author

Sato T, Abe T, Chida D, Nakamoto N, Hori N, Kokabu S, Sakata Y, Tomaru Y, Iwata T, Usui M, Aiko K, and Yoda T

Subjects
3T3 Cells, Acetylcholine analogs & derivatives, Acetylcholinesterase genetics, Alkaline Phosphatase metabolism, Animals, Blotting, Western, Cell Proliferation drug effects, Cells, Cultured, Choline O-Acetyltransferase genetics, Cholinergic Agents pharmacology, Cyclin D1 genetics, Cyclin D1 metabolism, Dose-Response Relationship, Drug, Mice, Osteoblasts cytology, Osteoblasts metabolism, Receptors, Cholinergic metabolism, Receptors, Muscarinic genetics, Receptors, Muscarinic metabolism, Reverse Transcriptase Polymerase Chain Reaction, Symporters genetics, Vesicular Acetylcholine Transport Proteins genetics, Acetylcholine pharmacology, Gene Expression Regulation drug effects, Osteoblasts drug effects, Receptors, Cholinergic genetics
Abstract

Recent studies have indicated that acetylcholine (ACh) plays a vital role in various tissues, while the role of ACh in bone metabolism remains unclear. Here we demonstrated that ACh induced cell proliferation and reduced alkaline phosphatase (ALP) activity via nicotinic (nAChRs) and muscarinic acetylcholine receptors (mAChRs) in osteoblasts. We detected mRNA expression of several nAChRs and mAChRs. Furthermore, we showed that cholinergic components were up-regulated and subunits/subtypes of acetylcholine receptors altered during osteoblast differentiation. To our knowledge, this is the first report demonstrating that osteoblasts express specific acetylcholine receptors and cholinergic components and that ACh plays a possible role in regulating the proliferation and differentiation of osteoblasts., (Crown Copyright 2010. Published by Elsevier B.V. All rights reserved.)

Published
2010
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