Search

Your search keyword '"Kamzolova SG"' showing total 2 results
Start Over Author "Kamzolova SG" Journal febs letters
2 results on '"Kamzolova SG"'

1. Physico-chemical study of complex formation of DNA with wild-type and mutant E. coli RNA polymerases. Recognition properties of beta-subunit.

Author

Ozoline ON and Kamzolova SG

Subjects
Carbon Radioisotopes, Electron Spin Resonance Spectroscopy, Mutation, Promoter Regions, Genetic, Protein Conformation, Temperature, Transcription, Genetic, DNA metabolism, DNA-Directed RNA Polymerases metabolism, Escherichia coli enzymology
Abstract

Complex formation of T7 DNA with RNA polymerase from E. coli B/r WU-36-10-11-12 (E. coli W12) and its rifampicin-resistant mutant rpoB409 was studied. The rpoB409 mutant possesses a highly pleiotropic effect due to alteration in the RNA polymerase beta-subunit structure. The two RNA polymerases have been previously shown to differ in gene selection during RNA synthesis on T7 DNA. In this study it was found that the change in selective properties of the mutant RNA polymerase occurs during its interaction with DNA, the general ability of the enzyme to melt DNA being unaffected.

Published
1986
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results