1. 1,25-dihydroxyvitamin D3 inhibits tenascin-C expression in mammary epithelial cells
- Author
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Manuel Álvarez-Dolado, Alberto Muñoz, and José Manuel González-Sancho
- Subjects
endocrine system ,Cell type ,Stromal cell ,animal structures ,Biophysics ,Breast Neoplasms ,Mammary epithelial cell ,Biology ,Tenascin-C ,medicine.disease_cause ,Biochemistry ,Extracellular matrix ,Mice ,chemistry.chemical_compound ,Breast cancer ,Calcitriol ,Downregulation and upregulation ,1,25-Dihydroxyvitamin D3 ,Structural Biology ,Tumor Cells, Cultured ,Genetics ,medicine ,Animals ,Humans ,RNA, Messenger ,RNA, Neoplasm ,Molecular Biology ,Tenascin C ,Tenascin ,Cell Biology ,musculoskeletal system ,Epithelium ,Neoplasm Proteins ,Gene Expression Regulation, Neoplastic ,medicine.anatomical_structure ,chemistry ,embryonic structures ,Carcinogens ,Cancer research ,Phorbol ,biology.protein ,Tetradecanoylphorbol Acetate ,Female ,Carcinogenesis - Abstract
Tenascin-C is an extracellular matrix protein with growth-, invasion- and angiogenesis-promoting activities. Tenasein-C is upregulated in breast carcinoma and stromal cells, and in many other cell types during tumorigenesis. We demonstrate that tenascin-C RNA expression is inhibited by 1,25-dihydroxyvitamin D3 (1,25-D3) in a variety of mouse and human mammary epithelial cell lines exhibiting normal or malignant phenotype. In EpH4 cells, the inhibition is maximum 24 h after 1,25-D3 treatment and correlates with a dose-dependent reduction in the synthesis of tenascin-C protein. Furthermore, 1,25-D3 also abolishes the induction of tenascin-C by serum or the tumor promoter 12-O-tetradecanoyl phorbol 13-acetate. The inhibition of tenascin-C expression may be relevant for the anticancer activity of 1,25- D3., This research was supported by Grants SAF95-0738 and SAF98-0060 from the Comisión Interministerial de Ciencia y Tecnología, Spain. J.M.G-S. was supported by a pre-doctoral fellowship from Comunidad Autónoma de Madrid.
- Published
- 1998