1. Neisseria meningitidis pili induce type-IIA phospholipase A2 expression in alveolar macrophages
- Author
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Françoise Thouron, Miguel Paya, Muhamed-Kheir Taha, Jean-Michel Alonso, Lhousseine Touqui, Annie Guiyoule, Maria Leticia Zarantonelli, Dominique Leduc, Yongzheng Wu, Défense innée et inflammation, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Neisseria, Institut Pasteur [Paris] (IP), L.T. was supported by the French non‐benefit association 'Vaincre la Mucoviscidose'., Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris], and Institut Pasteur [Paris]
- Subjects
Lipopolysaccharides ,MESH: Macrophages, Alveolar ,Lipopolysaccharide ,Mutant ,MESH: NF-kappa B ,Neisseria meningitidis ,medicine.disease_cause ,Biochemistry ,[SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity ,Pilus ,Nuclear factor kappa B ,chemistry.chemical_compound ,0302 clinical medicine ,Phospholipase A2 ,Structural Biology ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,MESH: Animals ,MESH: Phospholipases A2 ,0303 health sciences ,biology ,NF-kappa B ,Pili ,Arachidonic acid ,MESH: Phospholipases A ,Guinea Pigs ,Biophysics ,MESH: Neisseria meningitidis ,Phospholipases A ,Microbiology ,MESH: Fimbriae, Bacterial ,MESH: Guinea Pigs ,03 medical and health sciences ,Macrophages, Alveolar ,Genetics ,medicine ,Animals ,Molecular Biology ,030304 developmental biology ,Phospholipase A ,Macrophages ,NF-κB ,Cell Biology ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Phospholipases A2 ,chemistry ,Fimbriae, Bacterial ,biology.protein ,MESH: Lipopolysaccharides ,030215 immunology - Abstract
International audience; Induction of type-IIA secreted phospholipase A2 (sPLA2-IIA) expression by bacterial components other than lipopolysaccharide has not been previously investigated. Here, we show that exposure of alveolar macrophages (AM) to Neisseria meningitidis or its lipooligosaccharide (LOS) induced sPLA2-IIA synthesis. However, N. meningitidis mutant devoid of LOS did not abolish this effect. In addition, a pili-defective mutant exhibited significantly lower capacity to stimulate sPLA2-IIA synthesis than the wild-type strain. Moreover, pili isolated from a LOS-defective strain induced sPLA2-IIA expression and nuclear factor kappa B (NF-kappaB) activation. These data suggest that pili are potent inducers of sPLA2-IIA expression by AM, through a NF-kappaB-dependent process.
- Published
- 2005