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4 results on '"ASMase"'

1. Evidence for coordination of lysosomal (ASMase) and plasma membrane (NSMase2) forms of sphingomyelinase from mutant mice

Author

Qin, Jingdong and Dawson, Glyn

Subjects
*LYSOSOMES, *CELL membranes, *SPHINGOMYELINASE, *PHOSPHOCHOLINE, *FIBROBLASTS, *LIQUID chromatography-mass spectrometry, *SPHINGOMYELIN, *OSTEOGENESIS imperfecta, *LABORATORY mice
Abstract

Abstract: NSMase2 is associated to the plasma membrane, whereas ASMase is predominantly lysosomal; both hydrolyze sphingomyelin (SM) to ceramide and phosphocholine. Although SM accumulated in both ASMase−/− and fro/fro (NSMase2−/−) fibroblasts, the reduction of ceramides was more dramatic in fro/fro cells. ASMase mRNA, protein and enzyme activity were substantially elevated in fro/fro fibroblasts. In contrast, NSMase2 activity was unaffected in ASMase−/− fibroblasts. ASMase−/− cells showed normal cell cycling whereas fro/fro cells grew slowly and were arrested in G1/G0 and could be corrected by transfection with smpd3 gene. This suggests two distinct subcellular pathways for SM catabolism with distinct functions. [Copyright &y& Elsevier]

Published
2012
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2. Ceramide-rich platforms in transmembrane signaling

Author

Stancevic, Branka and Kolesnick, Richard

Subjects
*CERAMIDES, *CELL membranes, *CELLULAR signal transduction, *BIOPHYSICS, *PATHOLOGICAL physiology, *TARGETED drug delivery
Abstract

Abstract: Recent evidence suggests that ceramide regulates stress signaling via reorganization of the plasma membrane. The focus of this review will be to discuss the mechanism by which acid sphingomyelinase (ASMase)-generated ceramide initiates transmembrane signaling in the plasma membrane exoplasmic leaflet. In particular, we review the unique biophysical properties of ceramide that render it proficient in formation of signaling domains termed ceramide-rich platforms (CRPs), and the role of CRPs in the pathophysiology of various diseases. The biomedical significance of CRPs makes these structures an attractive therapeutic target. [Copyright &y& Elsevier]

Published
2010
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3. Ceramide-rich platforms in transmembrane signaling

Author

Richard Kolesnick and Branka Stancevic

Subjects
Ceramide, Platforms, Cell Membrane Permeability, Membrane Fluidity, Lipid Metabolism Disorders, Biophysics, Biology, Ceramides, Membrane Fusion, Biochemistry, Phase Transition, Article, chemistry.chemical_compound, Membrane Microdomains, Structural Biology, Genetics, Membrane fluidity, medicine, Animals, Humans, Molecular Biology, Mechanism (biology), Lipid bilayer fusion, Cell Biology, Lipid signaling, Transmembrane signaling, Lipid Metabolism, Cell biology, chemistry, ASMase, Acid sphingomyelinase, Signal transduction, Signal Transduction, medicine.drug
Abstract

Recent evidence suggests that ceramide regulates stress signaling via reorganization of the plasma membrane. The focus of this review will be to discuss the mechanism by which acid sphingomyelinase (ASMase)-generated ceramide initiates transmembrane signaling in the plasma membrane exoplasmic leaflet. In particular, we review the unique biophysical properties of ceramide that render it proficient in formation of signaling domains termed ceramide-rich platforms (CRPs), and the role of CRPs in the pathophysiology of various diseases. The biomedical significance of CRPs makes these structures an attractive therapeutic target.

Published
2010
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4. Evidence for coordination of lysosomal (ASMase) and plasma membrane (NSMase2) forms of sphingomyelinase from mutant mice

Author

Jingdong Qin and Glyn Dawson

Subjects
Male, Ceramide, Phosphorylcholine, Blotting, Western, Biophysics, Sphingomyelin phosphodiesterase, Biology, Ceramides, Biochemistry, Resting Phase, Cell Cycle, Article, Cell membrane, chemistry.chemical_compound, Mice, Structural Biology, Genetics, medicine, Animals, Sphingosine-1-phosphate, NSMase2, Molecular Biology, Cells, Cultured, Phosphocholine, Mice, Knockout, Mice, Inbred C3H, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Cell Membrane, G1 Phase, Cell Biology, Transfection, Cell Cycle Checkpoints, Fibroblasts, Flow Cytometry, Molecular biology, Sphingomyelins, medicine.anatomical_structure, Sphingomyelin Phosphodiesterase, chemistry, Coordination, Female, ASMase, Acid sphingomyelinase, Sphingomyelin, Lysosomes, medicine.drug
Abstract

NSMase2 is associated to the plasma membrane, whereas ASMase is predominantly lysosomal; both hydrolyze sphingomyelin (SM) to ceramide and phosphocholine. Although SM accumulated in both ASMase(-/-) and fro/fro (NSMase2(-/-)) fibroblasts, the reduction of ceramides was more dramatic in fro/fro cells. ASMase mRNA, protein and enzyme activity were substantially elevated in fro/fro fibroblasts. In contrast, NSMase2 activity was unaffected in ASMase(-/-) fibroblasts. ASMase(-/-) cells showed normal cell cycling whereas fro/fro cells grew slowly and were arrested in G1/G0 and could be corrected by transfection with smpd3 gene. This suggests two distinct subcellular pathways for SM catabolism with distinct functions.

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