Campbell, Davina E., Lengi, Andrea J., Akers, R. Michael, Daniels, Kristy M., Knowlton, Katharine F., Hill, Stephanie R., Pearson, Ronald E., and Corl, Benjamin A.
Stearoyl-CoA desaturase (SCD) is an endoplasmic reticulum enzyme responsible for production of monounsaturated fatty acids from saturated fatty acids and mainly utilizes palmitoyl- and stearoyl-CoA as substrates. The purpose of this study was to investigate the tissue distribution of SCD in calves and determine the effects of dietary fat. Calves (n=6/trt) were fed 1 of 4 milk replacers for 9 wks: daily intake of 0.4 kg/d 20% protein, 20% fat (20:20), 0.97 kg/d 28:20, 0.97 kg/d 28:28L, or 1.46 kg/d 28:28H. Average daily gains were 0.38, 0.61, 0.59, and 0.76 kg/d for 20:20, 28:20, 28:28L, and 28:28H, respectively. Stearoyl-CoA desaturase protein was detectable in subcutaneous adipose tissue (AT), perirenal AT, and omental AT but was not detectable in liver or small intestine samples in calves fed the 28:28H diet. Stearoyl-CoA desaturase protein abundance in subcutaneous AT was 4-fold greater in calves fed 28:28H diet compared to 20:20 diet and 2-fold greater compared to the 28:20 and 28:28L diets. In conclusion, SCD was not detected in liver or small intestine samples and AT SCD expression was increased in 28:28H diet. [ABSTRACT FROM AUTHOR]