1. Therapeutic silencing of miR-652 restores heart function and attenuates adverse remodeling in a setting of established pathological hypertrophy.
- Author
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Bernardo, Bianca C., Nguyen, Sally S., Winbanks, Catherine E., Xiao-Ming Gao, Boey, Esther J. H., Yow Keat Tham, Kiriazis, Helen, Ooi, Jenny Y. Y., Porrello, Enzo R., Igoor, Sindhu, Thomas, Colleen J., Gregorevic, Paul, Lin, Ruby C. Y., Xiao-Jun Du, and McMullen, Julie R.
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GENE expression , *MICRORNA , *HEART diseases , *HYPERTROPHY , *NUCLEIC acids - Abstract
Expression of microRNA-652 (miR-652) increases in the diseased heart, decreases in a setting of cardioprotection, and is inversely correlated with heart function. The aim of this study was to assess the therapeutic potential of inhibiting miR-652 in a mouse model with established pathological hypertrophy and cardiac dysfunction due to pressure overload. Mice were subjected to a sham operation or transverse aortic constriction (TAC) for 4 wk to induce hypertrophy and cardiac dysfunction, followed by administration of a locked nucleic acid (LNA)-antimiR-652 (miR-652 inhibitor) or LNA control. Cardiac function was assessed before and 8 wk post-treatment. Expression of miR-652 increased in hearts subjected to TAC compared to sham surgery (2.9-fold), and this was suppressed by ~95% in LNA-antimiR-652-treated TAC mice. Inhibition of miR-652 improved cardiac function in TAC mice (fractional shortening:29±1% at 4 wk post-TAC compared to 35±1% post-treatment) and attenuated cardiac hypertrophy. Improvement in heart function was associated with reduced cardiac fibrosis, less apoptosis and B-type natriuretic peptide gene expression, and preserved angiogenesis. Mechanistically, we identified Jaggedl (a Notchl ligand) as a novel direct target of miR-652. In summary, these studies provide the first evidence that silencing of miR-652 protects the heart against pathological remodeling and improves heart function. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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