1. Cryptosporidium species and Cryptosporidium parvum subtypes in dairy calves and goat kids reared under traditional farming systems in Turkey
- Author
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Jianbin Ye, Selma Usluca, Sibel Yasa-Duru, Aysegul Taylan-Ozkan, Colleen Lysen, Lihua Xiao, Yaoyu Feng, Dawn M. Roellig, Kırıkkale Üniversitesi, and Hitit Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü
- Subjects
0301 basic medicine ,Diarrhea ,Veterinary medicine ,Genotype ,Turkey ,animal diseases ,030231 tropical medicine ,Immunology ,Subtype ,Cattle Diseases ,Cryptosporidiosis ,Cryptosporidium ,Polymerase Chain Reaction ,law.invention ,03 medical and health sciences ,Feces ,0302 clinical medicine ,law ,parasitic diseases ,Animals ,Animal Husbandry ,Polymerase chain reaction ,Cryptosporidium parvum ,Goat Diseases ,biology ,business.industry ,Goats ,General Medicine ,Sequence Analysis, DNA ,030108 mycology & parasitology ,DNA, Protozoan ,biology.organism_classification ,Dairying ,Infectious Diseases ,Parasitology ,RNA, Ribosomal ,Goat ,Livestock ,Cattle ,business ,Polymorphism, Restriction Fragment Length - Abstract
Feng, Yaoyu/0000-0002-8380-1246; Ozkan, Aysegul Taylan/0000-0001-8421-3625; Feng, Yaoyu/0000-0002-8380-1246; Xiao, Lihua/0000-0001-8532-2727; TAYLAN-OZKAN, Aysegul/0000-0001-8421-3625 WOS: 000388059400003 PubMed: 27373430 Molecular characterizations of Cryptosporidium spp. in ruminants reared under traditional animal management systems are scarce and studies conducted thus far have revealed largely an absence of the pathogenic and zoonotic species Cryptosporidium parvum in pre-weaned animals. In this study, we examined Cryptosporidium species and subtype distribution in free-range pre-weaned dairy calves and goat kids with diarrhea. Cryptosporidium-positive specimens from pre-weaned calves on 10 farms and goat kids on 4 farms in Ankara, Balikesir, Corum, Kirikkale, and Kirsehir Provinces, Turkey were genotyped by PCR-restriction length polymorphism analysis of the small subunit rRNA gene, which identified C. parvum in 27 calves and 9 goat kids and Cryptosporidium ryanae in 1 calf. Among the C. parvum isolates successfully subtyped by DNA sequence analysis of the 60 kDa glycoprotein gene, three subtypes were detected in calves, including IlaA13G2R1 (20/23), IldA18G1 (2/23), and IldA20G1b (1/23), and four subtypes were detected in goat kids, including IlaA13G2R1 (3/8), IlaA15G1R1 (2/8), IldA22G1 (2/8), and IldA18G1 (1/8). Data of the study suggest that dairy calves reared in a traditional cow-calf system in Turkey are mainly infected with a C. parvum subtype rarely seen elsewhere, whereas goat kids are infected with diverse subtypes. As all five C. parvum subtypes found in this study are known human pathogens, pre-weaned farm animals could play a potential role in the transmission of human cryptosporidiosis. (C) 2016 Elsevier Inc. All rights reserved. National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31229005, 31110103901]; Centers for Disease Control and PreventionUnited States Department of Health & Human ServicesCenters for Disease Control & Prevention - USA This research was supported by the National Natural Science Foundation of China (Project No. 31229005 and 31110103901) and Centers for Disease Control and Prevention.
- Published
- 2016