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Your search keyword '"Morawiec-Sztandera A"' showing total 5 results
Start Over Author "Morawiec-Sztandera A" Journal experimental oncology
5 results on '"Morawiec-Sztandera A"'

1. Polymorphisms of the DNA repair genes XRCC1 and ERCC4 are not associated with smoking- and drinking-dependent larynx cancer in a Polish population

Author

Krupa, R., Kasznicki, J., Gajȩcka, M., Rydzanicz, M., Kiwerska, K., Kaczmarczyk, D., Jurek Olszewski, Szyfter, K., Blasiak, J., and Morawiec-Sztandera, A.

Subjects
Aged, 80 and over, Male, Alcohol Drinking, Smoking, Middle Aged, Short communications, Polymorphism, Single Nucleotide, DNA-Binding Proteins, X-ray Repair Cross Complementing Protein 1, Risk Factors, Case-Control Studies, Humans, Female, Poland, Laryngeal Neoplasms, Aged
Abstract

Tobacco smoking and alcohol drinking generate oxidative DNA damage and may contribute to larynx carcinogenesis. The X-ray repair cross complementing 1 (XRCC1) and excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4(XPF)) genes are important components of DNA excision repair systems, which repair DNA damage induced by various factors, including tobacco smoking and alcohol.To investigate the association between the genotypes of the XRCC1-Arg399Gln (rs25487) and ERCC4-Arg415Gln (rs1800067) polymorphisms and smoking- and drinking-related larynx cancer in a Polish population.The polymorphisms were determined by PCR-RFLP method in 253 patients with squamous cell carcinoma of the larynx and 253 sex- and age-matched controls.We did not find any association between the investigated polymorphisms and larynx carcinoma, dependent on either smoking or drinking status. No association was found between these polymorphisms and larynx cancer grade, stage or age at diagnosis.The results indicated that Arg399Gln polymorphism of XRCC1 gene and Arg415Gln polymorphism of ERCC4 gene may not be associated with smoking- and drinking-related larynx cancer in Polish population.

Published
2011

2. DNA double strand breaks repair and apoptosis induction in peripheral blood lymphocytes of head and neck cancer patients

Author

P, Rusin, L, Markiewicz, J, Olszewski, A, Morawiec-Sztandera, M, Kowalski, K, Przybylowska, D, Kaczmarczyk, K, Kusmierczyk, and I, Majsterek

Subjects
Male, DNA Repair, Cell Survival, Apoptosis, Hydrogen Peroxide, Middle Aged, Oxidants, Gamma Rays, Head and Neck Neoplasms, Carcinoma, Squamous Cell, Humans, DNA Breaks, Double-Stranded, Female, Comet Assay, Lymphocytes, Cells, Cultured
Abstract

To evaluate the generation and repair of DNA double strand breaks (DSBs) as a critical factors that define the efficiency of radiation therapy of cancer patients.Peripheral blood lymphocytes obtained from 18 patients with head and neck squamous cell carcinoma (HNSCC) and 18 healthy donors were studied. The efficiency of DSBs repair after genotoxic treatment with hydrogen peroxide and gamma-radiation were examined by neutral comet assay. MTT assay was used for cell viability analysis and Annexin V-FITC kit specific for kinase-3 was employed to determine apoptosis.Lymphocytes from HNSCC patients were sensitive to genotoxic treatment and displayed impaired DSBs repair. Finally, as a consequence of this finding we have evidenced higher rate of apoptosis induction after gamma-radiation treatment of lymphocytes from HNSCC patients than those from healthy controls.DSBs repair and increased apoptosis in cells of patients with head and neck cancer is relevant for efficient therapy of HNSCC.

Published
2009

3. Polymorphisms of the DNA base excision repair gene MUTYH in head and neck cancer

Author

T, Sliwinski, L, Markiewicz, P, Rusin, W, Pietruszewska, J, Olszewski, A, Morawiec-Sztandera, W, Mlynarski, and I, Majsterek

Subjects
Adult, Male, Genotype, DNA, Middle Aged, Polymorphism, Single Nucleotide, White People, DNA Glycosylases, Head and Neck Neoplasms, Risk Factors, Disease Progression, Humans, Female, Aged
Abstract

Head and neck squamous cell carcinomas (HNSCC) comprise about 6% of all malignant neoplasms. The major risk factors of -HNSCC are smoking and alcohol consumption. Genetic polymorphisms of DNA repair enzymes may lead to genetic instability and carcinogenesis. MUTYH gene encodes a DNA glycosylase that can initiate the base excision repair (BER) pathway and prevent G:CT:A transversion by excising adenine mispaired with 8-hydroxyguanine produced by reactive oxygen species (ROS).to perform a case-control study to test the association between polymorphism in the MUTYH gene: Tyr165Cys and head and neck cancer risk progression.Genotypes were determined in DNA from peripheral blood lymphocytes of 193 patients (among them 97 subjects with precancerous hyperplastic laryngeal lesions and 96 subjects with head and neck cancer) and 140 age, sex and ethnic-matched cancer-free controls by tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR).We found an association between head and neck cancer risk and the Tyr165Tyr variant of the MUTYH gene (OR 2.18; 95% CI 1.19-3.97). For Tyr165Tyr genotype we also observed positive correlation with cancer progression assessed by tumor size (OR 4.56; 95% CI 1.60-12.95). We did not observe any correlation between Tyr165Cys polymorphism of MUTYH gene and precancerous hyperplastic laryngeal lesions risk.The Tyr165Tyr polymorphic variant of the MUTYH gene may be associated with head and neck cancer in Polish population.

Published
2009

4. DNA double strand breaks repair and apoptosis induction in peripheral blood lymphocytes of head and neck cancer patients.

Author

Rusin P, Markiewicz L, Olszewski J, Morawiec-Sztandera A, Kowalski M, Przybylowska K, Kaczmarczyk D, Kusmierczyk K, and Majsterek I

Subjects
Carcinoma, Squamous Cell pathology, Carcinoma, Squamous Cell therapy, Cell Survival drug effects, Cell Survival radiation effects, Cells, Cultured, Comet Assay, Female, Gamma Rays, Head and Neck Neoplasms pathology, Head and Neck Neoplasms therapy, Humans, Hydrogen Peroxide therapeutic use, Lymphocytes drug effects, Lymphocytes radiation effects, Male, Middle Aged, Oxidants therapeutic use, Apoptosis, Carcinoma, Squamous Cell genetics, DNA Breaks, Double-Stranded, DNA Repair genetics, Head and Neck Neoplasms genetics, Lymphocytes pathology
Abstract

Aim: To evaluate the generation and repair of DNA double strand breaks (DSBs) as a critical factors that define the efficiency of radiation therapy of cancer patients., Methods: Peripheral blood lymphocytes obtained from 18 patients with head and neck squamous cell carcinoma (HNSCC) and 18 healthy donors were studied. The efficiency of DSBs repair after genotoxic treatment with hydrogen peroxide and gamma-radiation were examined by neutral comet assay. MTT assay was used for cell viability analysis and Annexin V-FITC kit specific for kinase-3 was employed to determine apoptosis., Results: Lymphocytes from HNSCC patients were sensitive to genotoxic treatment and displayed impaired DSBs repair. Finally, as a consequence of this finding we have evidenced higher rate of apoptosis induction after gamma-radiation treatment of lymphocytes from HNSCC patients than those from healthy controls., Conclusions: DSBs repair and increased apoptosis in cells of patients with head and neck cancer is relevant for efficient therapy of HNSCC.

Published
2009

5. Polymorphisms of the DNA base excision repair gene MUTYH in head and neck cancer.

Author

Sliwinski T, Markiewicz L, Rusin P, Pietruszewska W, Olszewski J, Morawiec-Sztandera A, Mlynarski W, and Majsterek I

Subjects
Adult, Aged, Disease Progression, Female, Genotype, Head and Neck Neoplasms epidemiology, Humans, Male, Middle Aged, Risk Factors, White People, DNA genetics, DNA Glycosylases genetics, Head and Neck Neoplasms genetics, Head and Neck Neoplasms pathology, Polymorphism, Single Nucleotide
Abstract

Background: Head and neck squamous cell carcinomas (HNSCC) comprise about 6% of all malignant neoplasms. The major risk factors of -HNSCC are smoking and alcohol consumption. Genetic polymorphisms of DNA repair enzymes may lead to genetic instability and carcinogenesis. MUTYH gene encodes a DNA glycosylase that can initiate the base excision repair (BER) pathway and prevent G:C > T:A transversion by excising adenine mispaired with 8-hydroxyguanine produced by reactive oxygen species (ROS)., Aim: to perform a case-control study to test the association between polymorphism in the MUTYH gene: Tyr165Cys and head and neck cancer risk progression., Methods: Genotypes were determined in DNA from peripheral blood lymphocytes of 193 patients (among them 97 subjects with precancerous hyperplastic laryngeal lesions and 96 subjects with head and neck cancer) and 140 age, sex and ethnic-matched cancer-free controls by tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR)., Results: We found an association between head and neck cancer risk and the Tyr165Tyr variant of the MUTYH gene (OR 2.18; 95% CI 1.19-3.97). For Tyr165Tyr genotype we also observed positive correlation with cancer progression assessed by tumor size (OR 4.56; 95% CI 1.60-12.95). We did not observe any correlation between Tyr165Cys polymorphism of MUTYH gene and precancerous hyperplastic laryngeal lesions risk., Conclusion: The Tyr165Tyr polymorphic variant of the MUTYH gene may be associated with head and neck cancer in Polish population.

Published
2009

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