1. Long term breeding of the Lmna G609G progeric mouse: Characterization of homozygous and heterozygous models
- Author
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Giovanna Lattanzi, Francesco Prisco, Mara Sanapo, Massimiliano Baleani, Elisa Schena, Catia Barboni, Cristina Capanni, Valeria Pellegrino, Anna Festa, Michela Murdocca, Roberta Fognani, Stefano Squarzoni, Alessia Diana, Orlando Paciello, Anna Zaghini, Maria Rosaria D'Apice, Serenella Papparella, Manuela Loi, Rosaria Mecca, Nikolina Linta, Giuseppe Sarli, Julie Rambaldi, Fabio Baruffaldi, Zaghini A., Sarli G., Barboni C., Sanapo M., Pellegrino V., Diana A., Linta N., Rambaldi J., D'Apice M.R., Murdocca M., Baleani M., Baruffaldi F., Fognani R., Mecca R., Festa A., Papparella S., Paciello O., Prisco F., Capanni C., Loi M., Schena E., Lattanzi G., Squarzoni S., Zaghini, A., Sarli, G., Barboni, C., Sanapo, M., Pellegrino, V., Diana, A., Linta, N., Rambaldi, J., D'Apice, M. R., Murdocca, M., Baleani, M., Baruffaldi, F., Fognani, R., Mecca, R., Festa, A., Papparella, S., Paciello, O., Prisco, F., Capanni, C., Loi, M., Schena, E., Lattanzi, G., and Squarzoni, S.
- Subjects
0301 basic medicine ,Quality of life ,congenital, hereditary, and neonatal diseases and abnormalities ,Aging ,Heterozygote ,Transgene ,Bone strength ,Biology ,Breeding ,medicine.disease_cause ,Biochemistry ,Kyphosi ,LMNA ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Endocrinology ,Progeria ,Genotype ,Genetics ,medicine ,Animals ,Molecular Biology ,Gene ,Mutation ,integumentary system ,Homozygote ,Membrane Proteins ,Cell Biology ,medicine.disease ,Lamin Type A ,Phenotype ,Disease Models, Animal ,030104 developmental biology ,Animal model breeding ,Hutchinson-Gilford Progeria Syndrome (HGPS) ,030217 neurology & neurosurgery ,Lamin - Abstract
The transgenic LmnaG609G progeric mouse represents an outstanding animal model for studying the human Hutchinson-Gilford Progeria Syndrome (HGPS) caused by a mutation in the LMNA gene, coding for the nuclear envelope protein Lamin A/C, and, as an important, more general scope, for studying the complex process governing physiological aging in humans. Here we give a comprehensive description of the peculiarities related to the breeding of LmnaG609G mice over a prolonged period of time, and of many features observed in a large colony for a 2-years period. We describe the breeding and housing conditions underlining the possible interference of the genetic background on the phenotype expression. This information represents a useful tool when planning and interpreting studies on the LmnaG609G mouse model, complementing any specific data already reported in the literature about this model since its production. It is also particularly relevant for the heterozygous mouse, which mirrors the genotype of the human pathology however requires an extended time to manifest symptoms and to be carefully studied.
- Published
- 2019