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Normal ageing is associated with an impaired systemic immune response contributing to an increased susceptibility to infectious diseases. The aim of this study was to compare the lymphocyte phenotype in human skin from old and young healthy subjects. Skin samples from donors were used for explant cultures before flow cytometric analysis. Our results depicted a higher proportion of CD4+ and a lower proportion of CD8+ among CD3+ T cells, a decreased proportion of CD45RA+ naive T cells (3.5 ± 1.9% vs 22.9 ± 11.1%, P ≤ 0.007) and an upregulation of the expression of CD39 and PD1 on CD3+CD4+ T cells (25.1 ± 8.5% vs 12.5 ± 8.5%, P ≤ 0.003, 68.8 ± 11.6% vs 50.0 ± 11.3%, P ≤ 0.01, respectively) in the skin of old subjects. These findings could explain a reduced generation of long‐lived memory T cells and an impaired antitumoral response in the skin of the elderly. [ABSTRACT FROM AUTHOR]

Patients included had to fulfil SLE criteria according to the American College of Rheumatology revised in 1997 and/or the 2012 SLICC4 and to have skin digits lesions similar to type 1 IFNpathies (including oedema, periungual and pulp erythema, and violaceous papules sometimes atrophic). Digital skin lesions in systemic lupus erythematosus (SLE) may be due to vascular changes (thrombosis or vasculitis) or specific lupus skin lesions mainly chilblain lupus or discoid lupus.1 Severe clinical changes of the digits observed in type 1 interferonopathies (IFNpathies) such as Aicardi-Goutières syndrome and familial chilblain lupus include purpura, oedema, periungual and pulp erythema, and violaceous papules. (C), Histogram representation (median and 95% CI) of relative mRNA expression of seven IFN-stimulated genes (MX1, IFIT1, IFI44, IFI27, ISG15, IFI44L, SIGLEC) in the digital lesion of two systemic lupus erythematosus patients (AP: acropulpitis) compared with healthy control skin (HC; n = 6). [Extracted from the article]

Normal ageing is associated with an impaired systemic immune response contributing to an increased susceptibility to infectious diseases. The aim of this study was to compare the lymphocyte phenotype in human skin from old and young healthy subjects. Skin samples from donors were used for explant cultures before flow cytometric analysis. Our results depicted a higher proportion of CD4 + and a lower proportion of CD8 + among CD3 + T cells, a decreased proportion of CD45RA + naive T cells (3.5 ± 1.9% vs 22.9 ± 11.1%, P ≤ 0.007) and an upregulation of the expression of CD39 and PD1 on CD3 + CD4 + T cells (25.1 ± 8.5% vs 12.5 ± 8.5%, P ≤ 0.003, 68.8 ± 11.6% vs 50.0 ± 11.3%, P ≤ 0.01, respectively) in the skin of old subjects. These findings could explain a reduced generation of long-lived memory T cells and an impaired antitumoral response in the skin of the elderly., (© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)