1. Overexpression of human acyl-CoA thioesterase upregulates peroxisome biogenesis
- Author
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Yukio Fujiki, Arata Takeuchi, Shinsuke Taki, Nobuyuki Nakajima, Sho Yamasaki, Masaru Miyazaki, Hiroyuki Watanabe, Takashi Saito, Yoshiro Toyama, Mitsuru Ishizuka, and Shigeki Yuasa
- Subjects
Peroxisome proliferator-activated receptor gamma ,T-Lymphocytes ,Fluorescent Antibody Technique ,Peroxisome Proliferation ,Mice, Transgenic ,Peroxisome localization ,Biology ,Gene Expression Regulation, Enzymologic ,Cell Line ,Jurkat Cells ,Mice ,Lipid droplet ,Peroxisomes ,Animals ,Humans ,Organelles ,Binding protein ,Cell Biology ,Peroxisome ,Catalase ,Lipid Metabolism ,Molecular biology ,Up-Regulation ,Microscopy, Electron ,Palmitoyl-CoA Hydrolase ,Thiolester Hydrolases ,Peroxisome proliferator-activated receptor alpha ,PPARGC1B ,Energy Metabolism ,Biomarkers - Abstract
The biological functions of human acyl-CoA thioesterase III (ACTEIII/PTE-1), initially identified as an HIV-1 Nef binding protein, have remained unclear. We report herein that the stable overexpression of ACTEIII/PTE-1 in human and murine T-cell lines resulted in an increase in both peroxisome number and lipid droplet formation in a manner dependent on the amount of the protein. Peroxisome proliferation was evidenced by immunofluorescence staining for catalase, a peroxisome marker protein, as well as by direct peroxisome enumeration on electron micrographs. Consistently, the amount of catalase was elevated as the amount of ACTEIII/PTE-1 was increased. ACTEIII/PTE-1 mutants with reduced enzymatic activity or with the defect in peroxisome localization did not induce peroxisome proliferation, indicating that peroxisome proliferation was mediated by metabolites generated by ACTEIII/PTE-1 within peroxisomes. Finally, thymocytes isolated from a T-cell-specific ACTEIII/PTE-1 transgenic mouse as well as human and murine cell lines of lymphoid and non-lymphoid origins exhibited a similar proliferation of peroxisomes. Thus, ACTEIII/PTE-1 may be involved in the metabolic regulation of peroxisome proliferation.
- Published
- 2004
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